细胞毒试验

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  • cytotoxicity test
细胞毒试验细胞毒试验
  1. 细胞毒试验表明对肝癌细胞具有杀伤作用,而对正常肝细胞无损伤。

    Cytotoxicity test showed the killing activity of expressed product to hepatocarcinoma cells but no damage to normal hepatic cells .

  2. 方法:体外细胞毒试验是用噻唑蓝(MTT)法。

    Methods : cytotoxicity test in vitro were measured by MTT assay .

  3. 方法:用MTT比色方法,细胞毒试验及细胞增殖实验,分析比较不同条件下MTT显色反应的性能特点。

    Methods : The colorimetric assay with MTT , and the experiments of cytotoxicity and proliferation of cells .

  4. 结果:1.细胞毒试验观察到Hcy促进平滑肌细胞生长与增殖;

    Results : 1.Homocysteine had promotive effect on the proliferation of aortic smooth muscle cells ;

  5. 应用H2Kb四聚体对特异性CTL检测结果与经典的细胞毒试验一致,同时对胞外IFNγ进行了定量检测。

    Specific CTL responses were demonstrated by H 2K b OVA tetramer staining and cytotoxicity assay . Extracelluar IFN γ was also quantitatively analyzed .

  6. 结论三次项曲线对MTS法细胞毒试验的细胞数OD关系有很高拟合度且优于线性曲线模型。

    CONCLUSION For the relations of cell number-OD in MTS cytotoxicity assay , the fitness of cubic curves are better than the linear model .

  7. 本文以NIH标准微量淋巴细胞细胞毒试验对上海地区所见41例白塞(Behcet)病进行了HLA-A·B位点检测。211例正常人作为对照。

    HLA-A and B locus were determined by NIH standard microlymphocytotoxicity test on serum from 41 patients with Behcet 's disease and 211 normals as a control .

  8. 82株不含有stx2或stx1基因大肠杆菌Vero细胞毒试验均阴性。

    Coli without stx 2 and stx 1 were negative in Vero cytotoxin assay .

  9. 方法:用51Cr释放法和MTT比色法、NAG比色法,单独或相互比较,检验分析细胞毒试验中效靶细胞相互作用规律,酶在细胞代谢中分布情况,细胞死伤前后酶释放特点。

    Methods : 51 Cr release way and the colorimetric assay of MTT and NAG used in the experiment of cell metabolism and cytotoxicity assay .

  10. 方法:用细胞毒试验、SDS-PAGE结合薄层扫描研究H.pylori临床分离株空泡毒作用及其与VacA的关系。

    Method : The vacuolated effect and its relationship to VacA of H.pylori clinical isolates were investigated by the method of cytotoxic test 、 SDS-PAGE and scan .

  11. 本实验用非同位素细胞毒试验方法观察了肿瘤坏死因子(TNFα)对宫颈来源的三个细胞系的体外杀伤作用,并比较重组人干扰素(IFNγ)对TNFα细胞毒性的协同作用。

    Experiments were designed to determine the cytotoxicity mediated by tumor necrosis factor ( TNF α) to three cervix derived cell lines in vitro . A synergistic increase in the cytotoxic effects of TNF α and interferon ( IFN γ) was analyzed .

  12. 采用补体依赖性细胞毒试验(CDC)和微量序列特异性引物聚合酶链反应(MicroPCRSSP)技术进行HLAI类和II类分型。

    Donors and recipients HLA class I typing was performed using complement dependent cytotoxicity ( CDC ) test with special monoclonal tray ( SMT ) and HLA class II gene typing by micro sequence specific primers polymerase chain reaction ( Micro PCR SSP ) .

  13. 细胞毒试验:96孔板每孔接种细胞,加不同剂量的药物培养72h,加入MTS培养3h后于490nm波长测定OD,用SPSS110软件对数据作曲线估计。

    The regression curves were estimated from the data with the software SPSS 11.0 . Cytotoxicity assay : Cells were seeded into 96 well plate and incubated with drug of different doses for 72 hours . OD was read at 490 nm after incubating with MTS for 3 hour .

  14. 改良微量细胞毒试验检测急性白血病外周血T细胞亚群

    Examination of T lymphocyte subsets of acute leukemia by improved microcytotoxicity assay

  15. 抗血清1∶320倍稀释后与鼠脾淋巴细胞做补体依赖的细胞毒试验,特异性细胞毒性均为32%。

    The antibody at a dilution of 1 ∶ 320 could kill about 32 % murine spleen cells in the complement dependent cytotoxicity assays .

  16. 马抗卵巢癌抗体体外细胞毒试验与~(131)碘标记抗卵巢癌抗体裸鼠卵巢癌移植瘤聚集性体内分布实验

    Study on in-vitro Cytotoxic Assay of Horse Anti-ovarian-cancer Antibody and Distribution of ~( 131 ) I-labeled Anti-ovarian-cancer Antibody in Murine Ovarian Cancer Transplanted Nude Mice

  17. 本文用抗胸腺细胞血清细胞毒试验检测了29例HBsAg无症状携带者外周血T细胞数,与正常人(20例)相比显著低下(P<0.001);

    29 cases of HBsAg asymptomatic carriers were assessed by cytotoxicity test using anti-thymus cell serum and by E-rosette test for the number of T cells in the peripheral blood .

  18. 酶释放法用于细胞介导的细胞毒试验问题分析

    The analysis of the problems of enzyme release way used for cell mediated cytotoxicity assay

  19. 为探讨细胞表面HLAⅠ类抗原分子作为细菌受体的可能性,用10属16种21株细菌,8份7个特异性的抗血清,对108份健康志愿者外周血淋巴细胞进行了细胞毒抑制试验。

    In order to determine the possibility of HLA class I antigen molecules as bacterial receptor , cytotoxicity inhibition test was carried out with 21 strains of bacteria , belong to 16 species of 10 genera of bacteria , 8 antisera of 7 specificity and 108 healthy volunteer lymphocytes .