细胞贴壁

羊水体积对细胞贴壁时间没有明显影响，但对贴壁细胞数量有显著影响（P0.05）。

The volume of amniotic fluid did not affect cell attachment time , but affected the cell attachment numbers to a certain extent ( P0.05 ) .

【结论】全骨髓培养结合细胞贴壁法可以成功的建立大鼠MSCs体外分离和培养体系，且培养的MSCs纯度高，活力强，性状稳定。

【 Conclusion 】 Combination of whole bone marrow culture and cell attachment way can successfully construct a rat MSCs isolation and culture system in vitro , and cultured MSCs with high purity , energy intensity , stable characters .

24h后有少量细胞贴壁；

24 hours later , there were some cells adherent to the wall ;

神经生长因子（NGF）可促进嗜铬细胞贴壁，存活和生长出神经轴突样突起，但是成人及成年大鼠肾上腺嗜铬细胞对于NGF的依赖程度存在种属差异。

NGF could enhance the attachment , Survival and neurite outgrowth , however , there appeared species differences .

24h后心肌细胞贴壁生长，细胞伸出伪足呈梭形、多角形；

24 hours later , the cells of fusiform and polygon began to adhere and stretch out the parapodium ;

但培养至4d后，胚胎卵泡细胞贴壁生长能力逐渐减弱并消失。

But the embryonal follicular cells lost the proliferative capability from the 4th day in culture .

【方法】无菌条件下取幼年SD大鼠的股骨，采用全骨髓培养结合细胞贴壁法分离纯化MSCs。

【 Method 】 sterile conditions , take young SD rat femur , combined with whole bone marrow culture adherent cell separation and purification of MSCs .

培养24h后部分细胞贴壁，呈椭圆形。

When the cells are training 24 hours , part of the cells begin to adherent and the shape of cells is oval .

铺被PLL明显促进兔髓核细胞贴壁和生长（1.12倍，p<0.01）；

Adhension and proliferation of rabbit NP cells were enhanced when coating the flasks with PLL ( 1.12 folds , p < 0.01 ) .

在此基础上，观察伤后1d伤口液对真皮多能干细胞贴壁力和迁移力等与创伤修复密切相关的指标变化。

The abilities of attachment and migration of dermal pluripotent stem cells stimulated by wound fluid of 1 st day were observed .

细胞贴壁时间：10h；

Time of cell attached : 10 h ;

传代后6～8h细胞贴壁生长。

After 6 to 8 hours ' subculture , the cells attached to the wall and sprawled out .

48h后更多细胞贴壁，呈梭形、多角形。

When the cells are training 48 hours , much more cells adherent , the shape of cells are Spindle , polygonal .

材料与方法1.1滑膜细胞贴壁培养及在明胶支架中培养：滑膜取自TMJ髁突肥大无滑膜病变的手术患者。分离出平滑光亮的滑膜组织，Ⅱ型胶原酶消化后。

Materials and Methods : Complex culture of cell and scaffold : The synovium is separated from joint in surgical patient of condylar hyperplasia .

缺氧24h再灌注12h中浓度清开灵组细胞贴壁良好，表面光滑，未发生皱缩，可见分裂期细胞。

In I-24-hour-R-12-hour MDQ group , cells were found well adhering to the wall , the surface was smooth without shrinkage and cleavage cells were observed .

SI对OB增殖、分化的影响：待细胞贴壁后，换加药培养基，作用48h及72h后，测定细胞MTT（OD）。

The effect of SI on OB in proliferation and differentiation : After cultured with SI 48h and 72 h , the MTT ( OD ) of OB were measured .

表明RH-CM能够促进ES细胞贴壁增殖和ES集落的形成，有效的维持ES细胞未分化状态。

It was showed that RH-CM not only could maintain ES cells in the undifferentiated state , inhibiting the spontaneous differentiation effectively , but also promote adherence and proliferation of mouse ES cells .

GT-2是一种较适合于草鱼细胞贴壁生长的微载体。

GT-2 is a suitable microcarrier for the growth of anchorage-dependent grass carp cells wall .

本文观察了骨髓造血基质细胞贴壁层及细胞悬液对GM-CFU-C生长的影响及照射后的变化。

The effects of adherent marrow stromal cell layer and stromal cells harvested in long - term liquid culture system on the growth of GM-CFU-C were investigated and compared .

第1代细胞贴壁率：传代细胞接种2h已有部分细胞贴壁，12h细胞基本贴壁完全，14h已有部分细胞开始增殖。

Attachment rate of the first passage : Two hours after the inoculation of passage cells , partial cells completed attachment , and all the cells completed the attachment at about 12 hours , and cell proliferation was found in some cells at 14 hours .

对培养过程中细胞贴壁过程、葡萄糖代谢速率、rHuEPO浓度及葡萄糖代谢速率与rHuEPO的浓度变化关系进行了实验研究分析和对比。

The analysis and comparison of the anchorage of CHO cells , metabolic rates of glucose , expression concentration of rHuEPO and the relation between metabolic rate of glucose and expression concentration of rHuEPO for culturing CHO cells on three different carriers were experimentally carried out .

细胞贴壁率最高达60%左右。

The highest cell attachment rate is about 60 % .

待细胞贴壁后，每孔进行换液。

After the adherent cells growth , medium was changed .

利用小鼠胚胎干细胞贴壁制备拟胚体的新方法研究

A novel method for preparing embryoid bodies with attached embryonic stem cells

显微镜下观察细胞贴壁情况及形态学变化。

Cell adhesion and morphological changes were observed under the microscope . 2 .

光镜观察见细胞贴壁生长减少，细胞密度变小。

The cellular density decreased under light microscope .

该细胞贴壁生长，具有接触抑制的性质。

These cells were able to grow adhering to the wall and stop growing with contact inhibition .

结果培养的细胞贴壁生长，融合后呈单层、镶嵌式排列；

Results The cultured cells grew against the wall of flask , formed a monolayer after fusion and showed a mosaic arrangement .

精原细胞贴壁较迟，贴壁后呈规则的圆形或卵圆形，以链状或团块状位于体细胞层之上，细胞分裂不完全，相互间以细胞间桥相连。

Then the spermatogonial cells , regularly round or ovoid in shape , adhered to the somatic cells in chains or clumps .

研究表明，牙囊细胞贴壁生长，并有集落形成能力，其表面有公认的干细胞表面标志物的表达，提示了牙囊细胞中间充质干细胞的存在。

It has been reported that dental follicle cells are plastic adherent and colony forming cells expressing the putative mesenchymal stem cell markers .