胸腺嘧啶核苷

胸腺嘧啶核苷与过渡金属配位稳定常数的测定

Determination of stability constants of thymidine and transition metal complexes

我们应用氚化胸腺嘧啶核苷研究了人的全血培养时影响淋巴细胞转化和同位素参入的一些基本因素。

Several factors affecting the lymphocyte transformation and the incorporation of tritiated thymidine in phytohemagglutinin ( PHA ) stimulated whole blood culture were investigated .

肝脏DNA合成率用3H标记胸腺嘧啶核苷（3HTDR）掺入法测得。

The rates of DNA synthesis were detected by 3H-TdR method .

人体胸腺嘧啶核苷激酶不完全cDNA的分离、结构分析和表达

Isolation , Preliminary Characterization and Expression of a Near Full-length cDNA for Human Thymidine Kinase

用氚&胸腺嘧啶核苷放射自显影方法，观察到RⅡ对小鼠前胃上皮细胞DNA合成有明显的抑制作用。

Autoradiography using H ~ 3-thymidine showed marked inhibitory effect of R ⅱ on DNA synthesis in the forestomach epithelium of mice .

用~3H-胸腺嘧啶核苷掺入抑制试验检测人外周血NK细胞活性

Study on inhibition of ~ 3h-tdr incorporation assay for NK cell activity of human peripheral blood

用胸腺嘧啶核苷双阻断法实现Vero细胞的同步化生长

Synchronous Growth of Vero Cells with Double Thymidine Block

60Coγ射线照射黄鳝血细胞，用~3H-胸腺嘧啶核苷（~3H-TdR）研究血细胞中DNA的影响。

The effect of 60Co Y-rays irradiation on the DNA of blood cells of Monopterus albus has been investigated by means of 3H-TdR incorporation .

在~3H-胸腺嘧啶核苷标记培养物神经元的实验中，观察到NGF可促进培养第3天的颈上节少量的神经元合成DNA。

In the experiments with 3H-thymidine incorporation , that the NGF may promote the synthesis of DNA in some neurons of the third day SCG in vitro was also observed .

方法采用细胞培养和3H-胸腺嘧啶核苷（3H－TdR）掺入技术。

Methods The proliferation of glomerular mesangial cells was detected by measuring 3H - thymidine ( 3H - TdR ) incorporation .

经3H－胸腺嘧啶核苷参入肝DNA法测定其生物活性。

The bioactivity of hHSS was determined by measurement of the rate of 3H-thymidine incorporation into the hepatocyte DNA after injection of hHSS into the abdominal cavity of partial hepatectomized rats .

方法：采用〔3H〕－胸腺嘧啶核苷和〔3H〕－脯氨酸掺入法，分别测定成纤维细胞增殖和胶原蛋白合成。

METHODS [ 3H ] - thymidine and [ 3H ] - proline uptake was adopted to measure fibroblast proliferation and collagen synthesis respectively .

本文用3H－TdR（氚－胸腺嘧啶核苷）掺入的方法，检测了68例大肠癌、24例乳腺癌、20例卵巢癌肿瘤细胞的体外药物敏感性。

In vitro drug sensitivity of 68 colon carcinomas , 24 breast carcinomas and 20 ovarian carcinomas was studied by 3H-TdR incorporation assay .

应用人血在体外接受14MeV快中子和~（60）Coγ线照射后进行培养，以~3氢-胸腺嘧啶核苷（~8H-TdR）作参入实验以反映DNA合成。

Human blood was exposed to 14-MeV fast neutrons and ~ ( 60 ) Co γ - rays in vitro , after which the blood was cultured . ~ 3H-TdR incorporation was followed in order to reflect DNA synthesis .

方法：应用细胞培养、四唑盐比色试验（MTT）、[3H]-胸腺嘧啶核苷（[3H]-TdR）掺入试验、Fluo-3和激光扫描共聚焦显微术测定细胞内Ca2+浓度等方法。

METHODS : Techniques of cell culture , MTT test , - TdR incorporation , fluo-3 and confocal laser scanning microscopy were used .

方法将小鼠骨髓基质干细胞进行分离和纯化培养后，加入EGF和PDGF-BB，利用3H胸腺嘧啶核苷（3H-TdR）掺入反映细胞增殖效果，倒置显微镜下观察细胞增殖及分化状态。

Methods : Murine bone marrow stromal stem cells were isolated and cultured in vitro before EGF and PDGF-BB were added in .

TP可逆性地催化胸腺嘧啶核苷转变为2-脱氧核糖-1-磷酸和胸腺嘧啶，即通过催化胸苷和胸腺嘧啶的相互转化而稳定细胞内胸苷的水平。

TP can catalyze reversible transformation of the thymidine to deoxyribose-1 - phosphate and thymine , thus stabilize the level of thymidine , which is the essential material for DNA synthesis .

用3H-胸腺嘧啶核苷（3H-TdR）标记变链菌的方法，检测矿化液对变链菌粘附的影响。

Tagging with 3H-TdR mutant of S.mutans to detect its effects on adherence of mutant of S.mutans and measure it with rate of adherence .

用MTT法和~3H-胸腺嘧啶核苷掺入法观察CTN-986对新生大鼠海马神经前体细胞增殖的影响，并初步探讨其作用机制。

By using MTT assay and 3H-thymidine incorporation assay , the effect of CTN-986 on neural progenitor cells proliferation was observed .

用浓度为10μg/ml的大黄素处理肺癌A-549细胞，测定了氚标记胸腺嘧啶核苷的DNA参入量。

The incorporation of [ ~ 3H ] - thymidine into DNA in lung cancer A-549 cells treated with emodin of 10 μ g / ml concentration was determined .

方法采用3HTDR掺入法，比较肿瘤细胞在经过CO2气腹、He气腹、Air气腹条件后，肿瘤细胞摄取胸腺嘧啶核苷合成DNA（CPM值）的情况。

Methods 3H-TdR incorporation was used to compare the DNA synthesis ( CPM value ) of tumor cells after exposure to simulated laparoscopic environments , composed of carbon dioxide , helium and room air , respectively .

分别于伤前、伤后6,12,24,48和72h处死动物，检测肠黏膜蛋白质含量、胸腺嘧啶核苷激酶活性、增生指数及PCNA表达。

Protein content , thy-midine kinase activity , proliferative index and PCNA expres-sion in the intestinal mucosa were analyzed at 0 , 6 , 12 , 24,48 , and 72 PBH respectively .

方法应用体外HSC培养技术，采用~3H-胸腺嘧啶核苷（~3H-TdR）掺入法测定HSC增殖；膜联蛋白（Annexin&V）/碘化丙啶（PI）双标记流式细胞术及透射电镜等方法测定HSC凋亡；

Methods 3H-thymidine incorporation , annexin-V / propidium iodide double-labeled flow cytometry ( FCM ) and transmission electron microscopy were employed to estimate the influence of RGDS on the proliferation and apoptosis of HSCs .

研究了胸腺嘧啶核苷（TdR）双阻断法同步化Vero细胞的最适条件，包括TdR处理浓度、TdR处理时间和两次阻断间的恢复生长时间。

The optimum conditions of synchronous growth of Vero cells with double thymidine ( TdR ) block were investigated , including the concentration of TdR , treatment time and recovery time .

各组在规定时间内心脏取血0.5ml，采用3H-胸腺嘧啶核苷（3H-TdR）掺入法测定鲫鱼（Carassiusauratus）淋巴细胞中3H-TdR掺入量（DPM值）。

0.5 ml blood samples were obtained from heart in testing time and the value of 3H-TdR incorporation of carassius auratus lymphocytes was measured by using 3H-TdR incorporation .

目的研究单纯疱疹病毒胸腺嘧啶核苷激酶/更昔洛韦（Herpessimplexvirusthymidinekinase/gancyclovir，HSVtK/GCV）自杀基因系统对视网膜母细胞瘤（retinoblastoma，Rb）细胞的体外杀伤作用以及旁观者效应发生的机制。

Objective To investigate the killing effect of herpes simplex virus thymidine kinase / gancyclovir ( HSV tk / GCV ) suicide gene therapy system on retinoblastoma ( Rb ) cells and the mechanism of bystander effect .

本文测定了不同浓度的甘草酸单铵（AG）对体外培养人和大鼠淋巴细胞的~3H胸腺嘧啶核苷（~3HTDR）参入抑制率及PGI2和TXA2合成的影响。

The influences of varied concentrations of AG on the 8H-TdR ( 3H-Thymidine ) incorporation inhibition rate and the release of PGI2 and TXA2 were studied in cultured human and rat lymphocytes .

目的：了解二氢嘧啶脱氢酶（DPD）和胸腺嘧啶核苷激酶（TK）的研究现状和在临床肿瘤治疗中的意义。

OBJECTIVE : To provide the advances of the research in dihydropyrimidine dehydrogenase ( DPD ) and thymidine kinase ( TK ) and the significances of their applications in clinical tumor chemotherapy .

目的：探讨胸腺嘧啶核苷磷酸化酶（TP）与子宫内膜癌的临床病理特征、无瘤生存率、微血管密度（MVD）的关系。

To study the relationships between the expression of thymidine phosphorylase ( TP ), the density of microvessels ( MVD ) of the tumor , the clinical and pathological features and tumor free survival rate in endometrial carcinoma .

应用~3H－胸腺嘧啶核苷参入法，测定可溶性生长因子（EGF、PDGF、TGF－β）等对体外培养人表皮角质细胞（EKC）增殖的影响。

The writers have investigated the effects of soluble growth factors ( EGF , TCG - β and PDGF ), insulin and PHA on the proliferation of EKCs in vitro using a ~ 3 H-thymidine incorporation assay .