膜片钳

对膜片钳技术在SDS发病机制研究中的应用前景作了展望。

The expectation about the application prospect of patch clamp technique on the mechanism of SDS were made .

Axon公司膜片钳系统的漏减分析

Leak subtraction analysis of axon patch clamp system

基于USB总线技术的全自动膜片钳系统数据采集控制接口的研制

Development of USB Bus Based Data Acquisition and Control Interface for Full-auto Patch-Clamp Systems

基于CPLD的膜片钳数据采集系统&UDA-I数据采集器

A CPLD-Based Data Acquisition System for Patch-clamp Experiments & UDA-I Data Acquisition Device

方法：应用膜片钳单通道技术记录苯妥英对缺氧大鼠大脑皮质神经元上K（Ca）通道电流活动。

Methods : K ( Ca ) channal current was recorded with patch clamp technique in rat corticocerebral hypoxic neurons .

全细胞膜片钳技术记录所需离子电流和AP。

The membrane currents and action potential were recorded by the whole-cell patch-clamp technique .

在逻辑控制模块中，通过采用FPGA新技术，来设计控制电路，实现对探头、膜片钳放大器主板的控制和参数设置；

In logic control , FPGA technology is applied to set the parameters of head stage and mainboard of patch-clamp .

方法膜片钳技术全细胞记录模式记录IK·Ca。

Methods Standard whole cell recording method was used .

方法采用酶急性分离的单个大鼠心室肌细胞，应用膜片钳全细胞记录技术，观察F2对ICa的影响。

METHODS Single ventricular cell of rat was obtained by enzymatic dissociation method .

通过基于RUP的UML系统建模方法对全自动膜片钳软件系统进行了分析和建模。

The automatic patch-clamp system had been analyzed and modeled by using the method of UML in RUP .

方法：应用膜片钳技术的内面向外式记录酶性分离的细动脉平滑肌上KATP通道。

METHODS : The currents of K ATP channels were recorded on inside-out patches of enzymatically isolated arteriolar smooth muscle cells .

方法酶消化法急性分离Wistar大鼠海马锥体神经元，采用全细胞膜片钳技术测定IK。

Methods Pyramidal neurons were enzymatically isolated from Wistar rat hippocampus .

方法采用脑片全细胞膜片钳记录技术，分别在电流钳和电压钳状态下记录大鼠SON神经元的膜电位和全细胞电流。

METHODS Using whole cell recording technique in brain slices , recorded the membrane potentials and whole cell currents of rat SON neurons were recorded respectively .

利用全细胞膜片钳技术，我们分析了大鼠视网膜GC上NMDA受体所介导的电流。

Using whole-cell recording made from retinal slice preparations , we first characterized NMDA receptor-mediated currents on GCs .

方法采用膜片钳全细胞技术记录红霉素对ADP及主要电流的影响。

METHODS Whole cell patch clamp techniques were used .

采用酶消化法为膜片钳实验提供大量SD大鼠原代胸主动脉平滑肌细胞。

Using enzymatic isolation method , we get a number of primary thoracic aortic smooth muscle cells of SD rats for patch clamp technique .

利用膜片钳（Patchclamp）技术研究根细胞质膜上通道特性的变化可以得到有关盐胁迫机制的许多有价值信息。

We can get much valuable information about the salt stress through studying the characterization of channels in plasma membrane by patch clamp technology .

结果经蛋白酶E或胰蛋白酶分离法得到的细胞在进行单细胞记录时虽然细胞的死亡率没有区别，但形态学、膜片钳等实验均表明蛋白酶E分离法分离的神经元活性更好；

Results Neurons isolated by pronase E were suitable for single record as examined by morphological observation and patch-clamp method , even though the death rates of neurons were no difference ;

通过使用全细胞膜片钳技术证明了在心室肌细胞上银杏苦内酯B可浓度依赖性的缩短动作电位时程，主要与增大延迟整流型钾电流有关。

Ginkgolide B may shorten the action potential duration in a concentration-dependent manner which mainly due to the increase of the delayed rectifier potassium current ( Ik ) in single ventricular myocytes by using patch-clamp techniques .

方法运用膜片钳技术的细胞吸附式方法记录NMDA通道的单通道电流。

Methods Cell-attached mode patch clamp technique was employed to record the single channel current of the NMDA receptor .

分离平滑肌细胞用膜片钳记录钙通道电流，观察BK对单个平滑肌细胞的影响。

Record the influence of BK in single smooth muscle cell by patch clamp amplifer .

方法：采用膜片钳技术的全细胞记录方式记录人肝癌细胞在不同浓度EGF中延迟整流性K＋通道的跨膜电流。

Methods : The transmembrane current of human hepatocyte carcinoma cells was recorded by the whole cell recording technique of patch clamp in different concentration of EGF .

方法在离体培养的A549细胞上，用膜片钳全细胞模式记录氯通道电流。

Methods The chloride channel currents were recorded in cultured A549 cells by using the whole-cell mode of patch clamp techniques .

利用全细胞膜片钳技术，采用胶原酶B急性分离的大鼠心室肌细胞，研究了牛黄酸和二甲基-氨氯吡咪对心肌细胞膜Na+/Ca2+交换电流的影响。

Using the whole cell patch-clamp technique to study effects of taurine and 5 - ( N , N-dimethyl ) - amiloride ( DMA ) on Na + / Ca 2 + exchange current in rat ventricular myocytes .

用膜片钳技术的全细胞方式在培养大鼠脑皮层神经元胞体膜上记录到电压依赖性钙通道电流（ICa）。

The voltage dependent calcium current ( ICa ) in cultured neurons from neonatal rat cortex was recorded with whole cell patch clamp technique .

目的研究神经元发育过程中离子通道电生理学特性的变化规律。方法用膜片钳记录仪记录体外培养海马神经元在不同发育时期NMDA受体离子通道电生理学特性的变化。

Objective : To study the electrophysiological characteristic of NMDA receptor ion channel on hippocampal neurons during their in vitro development .

方法利用内面向外式（inside-out）膜片钳技术。

Methods Membrane patch clamp technique on inside-out membrane patch .

本文将简要介绍膜片钳（Patchclamp）的原理和方法，以及它在平滑肌细胞膜离子信道的应用。

This little article will briefly introduce the technique of " patch clamp " and its application in the studies of ion channels in single smooth muscle cells .

在培养的大鼠颈上神经节神经元上，用全细胞膜片钳技术记录γ－氨基丁酸（GABA）所激活的通道电流。

Responses of cultured rat superior cervical ganglion ( SCG ) neurons to local application of GABA were studied by using whole-cell voltage-clamp technique .

本文作者应用连细胞膜片钳技术，观察了低剂量X射线全身照射对小鼠T淋巴细胞钙激活钾通道〔K（Ca）〕的影响。

The effect of low dose X-rays on Ca2 + - activated K + channels [ K ( ca ) ] in mouse thymic lymphocytes was observed using the cell-attached configuration of patch clamp technique .