菌丝体

简便易行的食用菌菌丝体基因组DNA提取法

A Simplified Method for Extracting DNA from the Mycelium of Edible Fungi

枯茗酸导致辣椒疫霉病菌菌丝体DNA代谢紊乱，使其含量增加。

Cumin acid could lead to mycelium DNA metabolic disorder , and increased its content .

鸡（土从）菌菌丝体却需要较低的CO2浓度方能生长良好，它更适宜于摇瓶培养。

The mycelia grow better on the static condition than on shaking culture .

腐生与共生食用菌菌丝体分离、培养及其DNA鉴定研究

Studies on the Isolation , Culture and DNA Identification of Mycelia of Saprophytic and Symbiotic Edible Fungi

冬虫夏草发酵菌丝体中主要核苷及核酸碱基的HPLC测定

HPLC Determination of Main Nucleoside and Nucleic Acid Base in Mycelia of Cordyceps

药用真菌菌丝体产SOD的研究初探

Primary Study on SOD Activity of Medicinal & Fungal Mycelium

发酵液的乙酸乙酯提取物和菌丝体的乙酸乙酯提取物对变形杆菌（Proteusspecies）均无活性。

Both ethyl acetate extractives were no inhibitory to Proteus species .

低温引起草菇菌丝体RNA变化的研究

Analysis of the Low Temperature Effect on the Change of V. volvacea Mycelium RNA

植物油加入到培养基后，促进了高度膨胀的菌丝体断片形成节孢子，同时增加了头孢菌素C的产量。

Plant oil added to the culture medium highly stimulated swollen hyphal fragments into arthrospore and enhanced the Cephalosporium acremonium to produce Cephalosporln C.

测定了白藜芦醇对番茄早疫病菌菌丝体细胞膜通透性、可溶性蛋白和DNA生成的影响。

The effects of resveratrol on cell membrane permeability , contents of soluble protein and DNA of A. solani were determined .

采用改良CTAB法成功提取了桦褐孔菌菌丝体DNA。

The DNA of Inonotus obliquus strains were extracted successfully by improved CTAB method .

GA与2.4－D对猴头菌丝体纤维素酶和多酚氧化酶活力的影响

GA and2 · 4-d effect on activities of polyphenol oxidase and cellulase in hyphae of hedgehog hydnum

以上述培养基配方和培养条件进行摇瓶发酵，得到发酵过程中菌丝体生物量、pH值、胞外多糖及胞内多糖含量的变化曲线。

Under the optimal fermentation condition we got the variation curves of mycelia biomass , pH value , extracellular polysaccharide and endocellular polysaccharide content .

为建立重组质粒DNA的快速鉴定方法，分别以阳性菌的菌斑、菌液的菌丝体以及质粒DNA为模板进行PCR鉴定重组质粒。

In order to establish a rapid method to identify the recombinant plasmid , the methods of PCR with templates from different sources were studied .

文章报告一种从灵芝菌丝体中获得较高质量RNA的方法。

A method used for the isolation and purification of total RNA from the hyphostroma of Ganoderma lucidum was reported .

同工酶分析结果表明，鸡腿蘑菌丝体酯酶（EST）同工酶在不同培养条件间存在差异。

Its esterase ( EST ) isozyme showed different patterns with different culture medium formulations .

此外工业实验还确定了云芝液体发酵的pH值、残糖浓度以及菌丝体形态等终点指标。

And the terminal criteria of the fermentation , such as pH value , the concentration of residual sugar and the shape of mycelia were designated .

同时，研究了菌株M1在液体发酵过程中菌丝体生物量和发酵液pH的动态变化等。

Growth curve of hypha and pH curve of fluid medium of strain M_1in liquid culture was studied also .

本文比较了11种药用及食用真菌菌丝体在马铃薯综合培养基中的生长情况，并测定了菌丝体中的SOD活力。

In this paper , the growth and SOD activity of 11 fungi mycelium in the unified potato culture medium were studied .

原发性肝癌组织中糖胺聚糖组成与结构的变化不同云芝菌株菌丝体多糖的单糖组分HPLC分析

The Composition and Structure Alterations of Glycosaminoglycan in Primary Hepatic Carcinoma ; HPLC analysis of monosaccharide compositions of mycelial polysaccharides from different Trametes versicolor strains

白金针菇的液体培养及6-BA对其菌丝体生长的影响

The liquid cultivation of flammulina velutipes and the effect of 6-BA on mycelium growth

本研究采用mRNA差异显示技术分离平菇生长发育过程中菌丝体和子实体的差异表达基因。

The differentially expressed genes between mycelium and fruit body of Pleurotus ostreatus were studied by the mRNA differential display RT-PCR technique ( DDRT-PCR ) in this study .

本文比较了不同浓度的糖蜜、马铃薯、麸皮、酵母膏以及温度和pH等因素对松茸菌丝体生长的影响。应用多因素试验正交优选法分析了影响松茸菌丝体生长的因素。

The effect of sirup , extract of potato and bran , yeast extract , temperature and pH on the growth of filaments of Tricholoma matsutake was determined by orthogonal test .

试验评价了影响吸附的4个因子：溶液pH值，振荡吸附时间，金属初始浓度和菌丝体投加量。

The 4 factors influencing the adsorption capacity of auricularia polytricha : pH value of solution , shaking time , initial concentration of metal ion and dosage of mycelium were appraised .

试验直接以固体培养基上的菌丝体为原料，采用尿素提取液提取菌丝体基因组DNA的方法，耗时短，步骤简捷，提高了效率和安全性。

In this paper , mycelia need not be cultured in liquid medium , and genomic DNA is extracted directly with urea extract . The method requires less time with higher efficiency and greater security .

其中，羊肚菌子实体干粉和由PDA液体培养基发酵产生的菌丝体冻干产物均不产生孢子。

Among them , morel fruiting body powder and freeze-dried product of PDA liquid medium fermentation of the mycelium did not produce spores .

抗盐碱性试验表明：2菌种有一定的抗盐碱能力，但pH和盐的双胁迫作用显然对菌丝体生长影响显著，菌丝体干质量下降较为明显。

The test also indicated that they had an ability of tolerance to salinity and alkalinity , but the mycelia dry mass dropped down evidently under a double stresses of pH and salt treatment .

免疫活性测试的结果显示子实体各组分在刺激小鼠脾淋巴细胞，T和B细胞的增殖，提高人外周血中NK细胞杀伤活性方面比菌丝体的作用强；

Effects of fractions of fruit body on stimulating the proliferation of spleen lymphocyte and T and B cells and activating the NK activity is stronger than that of fractions of mycelia .

除碳、氮源外，Vb1及K、P、Mg、S等元素也是金针菇菌丝体生长所必需的营养因子。适宜的碳氮比（C/N.）

VB1 and the elements K , P , Mg , S also were essential additives , in addition to carbon and nitrogen sources , for the mycelial growth .

灵芝和猪苓的抗癌活性物质是在固态培养的融合菌丝体中提取的液体成分，称为液体Khz。

Liquid Khz is liquid component that anti-cancer active substance of Ganoderma and Polyporus is extracted from the fusion mycelia in solid medium .