血浆凝固酶

对全部分离菌进行了系统鉴定，包括形态、染色、培养特性、生化特性、血清学反应、致病力、对抗菌剂的敏感性和血浆凝固酶试验等。

A systematic identification including staining , morphology , cultural characters , biochemical properties , serological reaction , pathogenicity , sensitivity to antimicrobial agents and plasma coagulase test was done for all isolates .

从30株血浆凝固酶阴性的葡萄球菌中分离13株耐甲氧西林血浆凝固酶阴性葡萄球菌（Methicilin－ResistantCoagulase－NegativeStaphylococciMR－CNS）。

13 Methicillin-Resistant Coagulase-Negative Staphylococci ( MR-CNS ) were identified from 30 Coagulase-Negative staphylococci .

目的探讨成都地区血浆凝固酶阴性葡萄球菌（CoNS）耐药性及分子流行病学特征。

Objective To investigate the antimicrobial resistance and molecular prevalence of coagulase-negative Staphylococcus ( CoNS ) in Chengdu .

目的研究血浆凝固酶阴性葡萄球菌（CNS）的生化反应特性，建立一种血浆凝固酶阴性葡萄球菌快速两步一补鉴定方法。

[ Objective ] To study biochemical characteristics of Coagulase-Negative Staphylococci ( CNS ) and develop a " two steps with one supplement " method for rapid identification of CNS species .

结果玻片法试验：枸橼酸钠抗凝人血浆凝固酶阳性率为98.53%，EDTAK2抗凝人血浆凝固酶阳性率为95.59%，二者差异无显著性（P0.05）。

Results In slide method , the positive rate of coagulase test was 98.53 % with sodium citrate as anticoagulant and 95.59 % with EDTA-K 2 as anticoagulant , respectively , there was no significant difference between two anticoagulants ;

用试管法做EDTAK2抗凝人血浆凝固酶试验，假阳性率最低，为3.33%，与枸橼酸钠抗凝人血浆的凝固酶试验假阳性率（10%）相比，差异有显著性（P0.05）。

The false positive rate ( 3.33 % ) was the lowest with test tube method and EDTA-K 2 as anticoagulant , there was significant difference in the false positive rate between EDTA-K 2 plasma and sodium citrate plasma ( P 0.05 ) .

对产致病性毒素金葡菌的检测一般采用血浆凝固酶实验和耐热核酸酶活性实验检测，对耐甲氧西林金葡菌的检测主要是通过Kerby-Bauer纸片扩散法进行检测。

On the production of pathogenic toxin of Staphylococcus aureus detection by coagulase test and thermostable nuclease activity in experimental testing , for methicillin-resistant Staphylococcus aureus was detected mainly by Kerby - Bauer paper .

成都地区血浆凝固酶阴性葡萄球菌耐药性及分子流行病学分析

Antimicrobial resistance and molecular epidemiology of coagulase-negative Staphylococci in Chengdu

血浆凝固酶阴性葡萄球菌鉴定及耐药谱分析

Routine identification and plasmid profile analysis for Coagulase-negative Staphylococci

基层医院血浆凝固酶阴性葡萄球菌感染现状和耐药性分析

Analysis of Nosocomial Infection and Antibiotic Resistance of Coagulase Negative Staphylococcus in a Primary Hospital

结论不同抗凝剂可影响玻片法、试管法血浆凝固酶试验的结果。

Conclusion Different anticoagulants can affect the results of slide method and test tube method coagulase tests .

结论柠檬酸钠和EDTANa2抗凝的兔血浆和人血浆检测葡萄球菌凝固酶均能得到正确结果，但EDTAK2抗凝的人血浆不能用于检测游离凝固酶。

Conclusion Correct results could be obtained using either human plasma or rabbit plasma containing EDTA . Na 2 and sodium citrate in determining staphylococcal coagulase , but human plasma containing EDTA . K 2 anticoagulant could not be used to detect free coagulase ( staphylocoagulase ) .

试管法试验：EDTAK2抗凝人血浆与枸橼酸钠抗凝人血浆凝固酶阳性率均为97.06%；

In test tube method , the positive rate of coagulase tests was 97.06 % with both sodium citrate and EDTA-K 2 as plasma anticoagulant .