质粒
- plasmid
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空肠弯曲菌质粒DNA提取条件的优化
A modified extraction method of the plasmid DNA in Campylobacter Jejuni
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小量提取质粒DNA的简易方法
A Simple Method Of Extracting Plasmid DNA On a Small Amount
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结果:pcDNA3.1-IL-10酶切鉴定的电泳结果显示,pcDNA3.1-IL-10质粒有一个560bp左右的插入片断,大小和IL-10cDNA大致符合
IL-10-cDNA fragment was obtained through cleaving pUC-T-IL-10cDNA by reconstriction enzymes .
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质粒DNA快速提取试剂盒(博大公司);
Kits for rapid extraction of plasmid DNA ( Boda Company );
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用Red介导的同源重组结合酶切改构质粒DNA
Reconstruction of plasmid DNA with Red-mediated homologous recombination combined with restriction digestion
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碱裂解法提取质粒DNA的研究
The Compare Study on the Method of Extracting Plasmid DNA
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并用裸DNA质粒经肌肉接种鸡体内表达。
Chickens were immunized intramuscularly with the naked DNA plasmid .
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大肠杆菌质粒DNA提取方法的优化
A Modified Method for Isolation of Plasmid DNA from E.coli
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通过质粒双酶切和DNA测序证实该重组质粒构建正确。
This was confirmed by cleavage of restriction endonuclease and DNA sequencing .
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在碱解法的基础上,优化了提取空肠弯曲菌质粒DNA的条件。
The conditions of extracting plasmids DNA in Campylobacter jejuni were optimized .
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一种从质粒DNA中去除细菌染色体DNA污染的方法
A method of eliminating chromosomal DNA from contaminated plasmids DNA
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快速密度梯度离心法纯化质粒DNA
Purification of plasmid DNA by rapid density gradient centrifugation
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对重组质粒DNA进行纯化,并对目的基因片段进行核苷酸序列分析。
The recombinant plasmid was purified and the target DNA fragment was sequenced .
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建立一种全质粒DNA序列测定方法
To construct a method of whole plasmid DNA sequencing
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重组质粒DNA中插入子的核苷酸序列分析
Nucleotide sequence analysis of inserts in recombinant plasmid DNA
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原子力显微镜研究不同温度下质粒DNA结构变化
Plasmid DNA structures in different rangs of temperature studied by atomic force microscopy
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大质粒DNA提纯方法的改进
Improved Method for the Extraction of Large Plasmid DNA
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连续热裂解法大规模制备质粒DNA
Large-scale Plasmid DNA Preparation by Flow-Through Thermal Lysis
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医院内耐药细菌质粒DNA的实验研究
Experimental research of plasmid profile of drug resistance
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提取质粒,基因组DNA进行PCR扩增测序。
Plasmid was extracted and genome DNA was sequenced by PCR amplification .
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激光辐照对离体质粒DNA的断裂效应
Effect of Laser Radiation on Single and Double Strand Breakage of in vitro Plasmid DNA
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不锈钢血管支架蛋白涂层和携带质粒DNA的研究
Methodological Studies of Protein Coating and Plasmid DNA Delivery Based on Stainless Steel Coronary Stents
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四川省规模化猪场大肠杆菌质粒DNA分析
Plasmid DNA analysis on E coli from the large scale of pig farm in Sichuan Province
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TGF-β1质粒DNA前房注射在角膜内皮细胞表达的实验研究
Expression of Plasmid DNA Encoding TGF - β _1 in Endothelium after Injection into the Anterior Chamber
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基于Vector类向量运算的碰撞侦测研究将其克隆到E.coli质粒上进行序列分析。
Analysis of Collision Detection Based on Vector Quantities coli vector .
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扩增产物经纯化后直接克隆到PMD18-T载体系统中,经PCR及酶切法鉴定,所得的重组质粒中含有495bp的片段。
After purification , the DNA fragment was cloned into PMD-18T vector directly .
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鼠疫菌66Mdal质粒与鼠毒素及F1抗原的关系
The relationship between plasmids of y.pestis , and murine toxin & F1 antigen
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方法:质粒提取及PCR扩增。
Methods : Plasmid extraction and PCR amplification .
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经研究发现纯化的质粒不含RNA和蛋白质污染;
The purified plasmid DNA did not contaminate with RNA and protein .
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我国类鼻疽流行病学调查云南省鼠疫疫源地鼠疫菌质粒DNA种类及分子流行病学研究
Study on the Category of Plasmid DNA and Molecular Epidemiology of Yersinia pestis Isolated from Yunnan Natural Plague Foci