质粒载体

NOS/HuIFNa嵌合基因的构建及其与Ti质粒载体的拼接

Construction of nos / huifna chimeric gene and its fusion with Ti plasmid vector

cathepsinK基因siRNA的筛选及其质粒载体的构建

Experimental Study on siRNA Screening of Cathepsin K Gene and Construction of Its Plasmid Vector

CpG寡脱氧核苷酸长链DNA的克隆及重组质粒载体的构建

Cloning and construction of recombinant plasmids containing long strand CpG oligodeoxynucleotides DNA

Fas靶向RNA干扰质粒载体的构建及生物活性鉴定

The construction and identification of Fas-targeting siRNA - expressing plasmid

新城疫病毒F（48）E9株病毒F基因表达盒的火鸡疱疹病毒转移质粒载体构建

Construction of recombinant HVT transferring plasmid vector with fusion gene cassette of NDV F _ ( 48 ) E_ 9 strain

缺陷型TGF1βⅡ型受体真核表达质粒载体的构建

The construction of the vector of truncated type ⅱ receptor of TGF β _1 eucell expressing plasmid

本文为农杆菌Ti质粒载体介导的外源基因向植物中的转移提供了一个简便易行的受体系统。

This paper offered a advantageous recipient system for transfer of foreign genes into plant mediated by Agrobacterium Ti plasmid .

目的构建HAb18G反义RNA表达质粒载体。

AIM To construct eukaryotic vector expressing antisense RNA of HAb18G .

以胚肝RNA逆转录产物为模板，PCR扩增TβRⅡ胞外区基因，克隆到pGEM-Teasy质粒载体上。

Reverse transcriptase Extra-cellular domain gene was amplified by PCR , and cloned to the pGEM ~ T easy plasmids vector .

一种快速构建单靶或双靶基因位点RNAi质粒载体的方法

A strategy for rapidly constructing the single and dual-site targeting plasmid-based RNAi vector

DNA序列分析中克隆片段在M13的缺失及质粒载体的应用

Deletion of Cloned DNA Fragment in M13 and Application of Bluescript Vector for Subcloning and Sequencing

风疹病毒E1特异肽段的重组质粒载体构建

Construction of Plasmid Expression Vector for Specific Peptide of the Rubella Virus E1 Gene

FasL质粒载体构建及在小鼠心脏移植中抗排斥反应的研究

The Research on the Construction of FasL Plasmid Vector and Its Anti-rejection Effect in Mice Heart Transplantation

目的：确认变形链球菌表面蛋白pac基因A区片段重组表达质粒载体pET-17b-A中的A区片段阅读框正确。

AIM : To confirm and correct the open reading frame of pac gene 's A region in pET-17b-A.

35S启动子组入蓝藻质粒载体pUC13－pbl

Insetting of 35S promoter into the plasmid vector PUC 13-pb1 of the cyanobacteria

目的通过构建能产生长双链RNA的质粒载体，探讨长双链RNA非特异性诱导人骨肉瘤细胞凋亡的可行性。

Objective To construct the plasmid that can produce long , double-stranded RNA and identify nonspecific apoptosis induced by long , double-stranded RNA in human osteosarcoma cells .

目的构建pcmGMCSF重组质粒载体，为mGMCSF基因治疗肿瘤的研究奠定基础。

Objective To construct recombiant plasmid pc-mGM-CSF and lay a primary foundation for further study of mGM-CSF gene therapy for tumors .

目的：将幽门螺杆菌（Helicobacter，pylort，Hp）鞭毛蛋白B亚单位基因（flaB）克隆到质粒载体pNEB193上，并进行序列分析，为Hp疫苗的构建奠定基础。

Objective : To clone Helicobacter pylori ( H.pylori ) flaB into plasmid and analyze gene sequence , and to lay foundation for Hp vaccine .

抑制效率的差异可能与反义基因片段的位置、长短以及反义RNA表达质粒载体有关。

The reasons for the differ-ences in inhibition effects may be resulted from the sizes and positions of the HCV anti-sense gene fragments or the as RNA expressing vectors .

方法采用PCR技术从人胎盘和肺cD-NA文库中扩增出全长人TRAIL基因，克隆到pUC19质粒载体上进行测序。

Methods The intact full human TRAIL gene was amplified using PCR method from the human placenta and lung cDNA library .

此外，我们还探讨了诱导前免疫致敏对T细胞疫苗活性的影响，并比较了HCV腺病毒载体和质粒载体在此免疫致敏功能方面的差异。

Furthermore , we discussed the influence of immune-sensibilization before induction to the activity of T-cell vaccine , compared the immune-sensibilization difference between HCV-adenovirus vectors and plasmid vectors .

目的：构建一个新型的携带有人内皮一氧化氮合酶（eNOS）cDNA的质粒载体并研究其体外表达，以用于基因治疗。

AIM : To construct an AAV based vector carrying human endothelial nitric-oxide synthase ( eNOS ) cDNA and study its expression in vitro for future gene therapy .

方法重组报告事因加强的绿色荧光蛋白（EGFP）基因至质粒载体pSub201，用双质粒共转染方法制备重组腺病毒相关病毒颗粒（rAAV／EGFP），观察其对肝癌细胞系的感染性。

Methods The rAAV / enhance green fluorescein protein ( EGFP ) recombinant was prepared by the routine method of two plasmids cotransfection .

百脉根子叶外植体被含发根性Ri质粒载体的发根农杆菌感染，孩载体带有一个npt－Ⅱ基因和一个甘露碱合成酶基因。

Cotyledon explants of Lotus corniculatus ( Leo ) was infected with Agrobacterium rhizogenes containing Ri plasmid carring a kanamycin resistant gene npt-II and a mannopine synthase gene .

方法：将Bcl-xLsiRNA质粒载体转染至A549/DDP细胞，MTT、流式细胞仪检测细胞药物敏感性的改变；

METHODS : Bcl-XL siRNA and negative siRNA plasmid vector were stably transfected into A549 / DDP cells . Drug sensitivity of the cells to cisplatin ( DDP ) was analyzed with MTT and flow cytometry .

结果：成功构建hGH转基因家蝇表达质粒载体pchGH和pcFhGH。

Results : The expression vectors ( pchGH and pcFhGH ) of hGH for transgenic housefly was constructed .

结论：非病毒质粒载体pRC/RSV介导的人凝血因子Ⅷ基因能够在小鼠32D细胞系中表达人FⅧ蛋白，所表达的FⅧ与正常人血浆中的野生型FⅧ具有相似的凝血活性。

It is concluded that the recombinant plasmid RC / RSV hF ⅷ BDcDNA can successfully express human F ⅷ in mouse 32D cell line , and hF ⅷ expressed in vitro presents the similar coagulant activity to the native hF ⅷ existing in normal human plasma .

目的：构建一个含有增强型绿色荧光蛋白（EGFP）报告基因，并能够用于鼠粒细胞巨噬细胞集落刺激因子（mGM-CSF）基因修饰性肿瘤疫苗研究的质粒载体。

Objective : To construct a plasmid containing an enhanced green fluorescence protein ( EGFP ) reporter gene as the effective vector for preparing murine granulocyte macrophage colony stimulating factor ( mGM-CSF ) secreting tumor vaccines .

结果IFNα-2b基因克隆到表达质粒载体pGAPZα-A并成功地实现了在酿酒酵母中的表达，该目标蛋白不需复性，其活性可达到1.0×109IU/mg蛋白。

Results IFN α - 2b genes were cloned to the plasmid expression vectors pGAPZ α - A and successfully expressed in S. cerevisiae ; the target protein required no renaturation and its activity reached 1.0 × 109IU / mg protein .

为了使大肠杆菌代谢葡萄糖和木糖产乙醇，将运动发酵单胞菌乙醇发酵途径中的关键酶基因丙酮酸脱羧酶基因与质粒载体pGM-T连接转化至大肠杆菌TOP10中。

To construct a recombinant Escherichia coli strain is able to metabolize glucose and xylose to ethanol , pyruvate decarboxylase gene encoding essential enzyme of the fermentative pathway for ethanol production in Zymomonas mobilis was inserted to plasmid pGM-T and transform into E.