陷窝

结果RANK的阳性信号出现在乳牙根吸收面的破牙细胞和接近牙胚的牙槽骨陷窝中的破骨细胞、恒牙胚的成牙本质细胞和前期牙本质中。

Result RANK positive signals were observed on the odontoclasts in the absorbed lacunae of deciduous teeth roots and osteoclasts in the alveolar bone near .

免疫组化结果显示，RANKL和OPG的阳性表达位于牙周成骨细胞、骨衬里细胞、牙周纤维细胞和位于骨陷窝内的破骨细胞中。

Immunohistochemical staining revealed that expression of RANKL and OPG protein was detected in osteoblasts , bone lining cells , fibroblasts and osteoclasts which mostly located in resorption lacunae .

B组软骨细胞位于软骨陷窝中，细胞器较C组查差，可见线粒体空泡，有脂滴形成。

Group B , the cartilage cell in cartilage lacuna , the organelle is less than group C.

B组第8wk镜下可见股骨头内脂肪细胞增多，骨细胞坏死空骨陷窝形成明显；

At the end of the 8th week in group B , empty lacuna , increased adipocytes and osteocyte necrosis were extremely obvious ;

20周透射电镜结果：C组软骨细胞位于软骨陷窝中，细胞器发达，粗面内质网成扩张状态。

The result of electronic microscopic view after 20 weeks : Group C , the cartilage cell in cartilage lacuna , with advanced organelle and expanded ?

组织学检查C组修复软骨与周围软骨融合较好，可见较多的透明软骨细胞及软骨陷窝；

Histology examination The new cartilage in C group was bonded integratedly with the perimeter articular cartilage , a lot of hyaline chondrocyte and cartilage lacuna might be seen in these tissues .

目的观察氟对大鼠破骨细胞（Osteoclast，OC）形成的骨吸收陷窝的影响。

Objective To study the effect of fluoride on resorption pit of osteoclast .

通过抗酒石酸酸性磷酸酶（TRAP）染色、降钙素受体（CTR）免疫荧光检测、骨吸收陷窝形成方法鉴定破骨细胞。

Osteoclast formation was evaluated in tartrate resistant acid phosphatase ( TRAP ), calcitonin receptors ( CTR ) and bone-resorption activities .

II型胶原免疫组化染色：各组染色均为阳性，软骨细胞胞浆内及软骨陷窝周围深染。

The immunohistochemical staining of type II collagen : there was positive staining in all groups . The staining was heavy in the cytoplasm of cartilage cells and aroud the cartilage lacuna .

脾胃虚弱型和脾肾阳虚型以陷窝溃疡为主（P0.01）。

Mucosal ulcer is dominant in damp-heat syndrome while crypt ulcer is dominant in the types of spleen-stomach asthenia and spleen-kidney Yang deficiency ( P 0.01 ) .

采用细胞化学法对破骨细胞actin进行标记，采用分光光度计测量Ca和P离子浓度，采用扫描电镜（SEM）观察破骨细胞的形态及吸收陷窝。

Osteoclast actin labeling was examined by cytochemical staining . The concentrations of Ca and P in culture medium were quantified by colorimetric methods . SEM examined osteoclast morphology and resorption lacuna .

结果成骨细胞呈饱满的梭形，ALP染色阳性；破骨细胞呈多核，TRAP染色阳性，可以吸收骨质形成骨陷窝。

Results Osteoblasts are full fusiform , ALP stain positive cells , while osteoclasts are multinuclear and TRAP stain positive , with the ability of forming bone lacuna ;

并且PMMA±BaSO4组TRAP阳性的MNCs及虫蚀样骨吸收陷窝的形成均较对照组早。

The time of appearance of TRAP positive MNCs and lacuna resorption pits was also earlier in PMMA ± BaSO_4 groups compared to that of the control group .

方法采用体外成熟破骨细胞和大鼠颅盖骨培养体系，检测新伐他汀作用7d后破骨细胞骨吸收陷窝和培养上清钙的变化；

Methods The bone resorption activities of the osteoclast were evaluated after treatment of Simvastatin for 7 days in vitro ;

运用甲苯胺蓝染色法观察含药血清对成骨细胞-破骨细胞共育体系中OC形成骨吸收陷窝的影响。

Determine the effect of BBC-containing serum on absorption of lacuna formed by OC in osteoblast and osteoclast co-culture system by toluidine blue staining .

A组核溶解骨细胞数、空骨陷窝数、凋亡指数明显低于B组（P<0.01），略高于C组（P>0.05）；

The number of empty lacunae , karyolysis , pyknotic nuclei of osteocytes and apoptotic index of group A were lower than those of group B ( P < 0.01 ) and a little higher than those of group C ( P > 0.05 ) .

抗酒石酸酸性磷酸酶（TRAP）染色观察OC形态变化，图像分析体外骨吸收陷窝面积，原子分光光度计法测定OC培养液上清液中Ca2+含量。

The OCs were observed by the TRAP stain . The area of bone resorption and the concentration of Ca 2 + in the supernatant liquid of OCs cultures were also tested .

结果：在全血高切、中切、低切粘度、血浆粘度、总胆固醇、甘油三酯、TXB2及股骨头空骨陷窝率的比较上，治疗组均低于模型组（P＜0.01）；

Results : The data of the blood viscosity , plasma viscosity , Cholesterol , triglyceride , TXB_2 and the number of empty lacuna in treat group were lower than those of model group ( p0.01 );

结果随着培养液内alendronate浓度增高，骨吸收陷窝数减少，面积亦减少。而用alendronate浸泡过的骨片上未见骨吸收陷窝。

The results showed that the number and area of bone resorptive pits were not found on bone slices that once incubated in alendronate solution .

牙囊周围及接近牙胚的牙槽骨骨质吸收活跃，陷窝内可见多核OC，恒牙胚冠部牙本质、牙釉质进一步形成和矿化。

The multinucleated osteoclasts were localized in the absorbed lacunae of the alveolar bone near the germs . Dentin and enamel of the permanent teeth germs went on forming and calcifying .

结果：（1）MC为颅中窝后部的硬脑膜陷窝，包含硬膜下腔、蛛网膜、蛛网膜下腔、三叉神经节、神经纤维和脑脊液等结构；

Results : 1 . MC was a extradural recess in the posterior of the middle fossa , it contained subdural space , arachnoid , subarachnoid space , trigeminal ganglion , neural fibers and cerebrospinal fluid , et al ;

方法自1d龄SD大鼠四肢长骨获得破骨细胞，接种于象牙薄片上，于培养第7d取出，计数各组骨吸收陷窝的数量。

Method Get osteoclasts from one-day-old SD rats ' long bones , vaccinate them on thin ivory slices , get out at the 7th day of culture , count the bone absorptive lacunae in each group .

Meckel腔（MC）为颅中窝后部的硬脑膜陷窝，位置深在、结构复杂并与许多重要神经、血管结构相毗邻。

Meckel cave ( MC ) is a dural recess in the posterior of the middle fossa , depth in location , complex in structures , and adjacent to many important nerves and blood vessel .

实验组35、70d双侧股骨头软骨下区骨陷窝空虚率均较对照组显著增高。

Empty bone lacunae were higher in group B than in group A in 35 and 70 days .

结果B组培养5、7d抗酒石酸酸性磷酸酶（TRAP）染色阳性多核OLC数量及TRAP活性均高于A组，骨吸收陷窝计数亦较A组为多，差异均有统计学意义（P<0.01，P<0.05）；

Result The amount of TRAP-positive multinucleated OLCs and bone absorptive lacunae , the activity of OLC TRAP in group B were higher than group A at day 5 and day 7 ( P < 0.01 , P < 0.05 ) .

PMMA±BaSO4组象牙磨片上骨吸收陷窝的面积均较对照组大，差异具有显著性（P<0.01）；

Result : There was no difference in the number of TRAP positive MNCs among the groups , while lacuna resorption area on dentine slices in PMMA ± BaSO_4 groups significantly increased compared to the that of control group ( P < 0.01 ) .

活体观察OLC形成情况并于培养的第6天行细胞染色，以抗酒石酸酸性磷酸酶（TRAP）染色（+）、细胞核≥3个的细胞为OLC，计数各组OLC及骨陷窝。

At 6-th culture day , OLC was identified by the characteristics of TRAP staining ( + ) and three nuclei or more . The number of OLCs and bone resorption pits were measured under microscopy .

D组所形成OLC及骨陷窝数目较其它3组明显增多（P<0.01），且OLC出现较早。

Comparing with other 3 groups , the number of OLCs and bone resorption pits in the group D were obviously increased ( P < 0 01 ) and cells appeared relatively earlier .

组织学观察：A组4w可见大量成骨细胞，少量软骨细胞，形成骨陷窝结构。8w时可见骨细胞成熟，骨小梁结构出现，髓腔形成。

Histologic observation shew that in group A , there were many osteoblast , a few cartilage cells , and bone lacuna in 4w.In 8w , osteocyte were mature , and bone trabecula can be seen .

为了比较补肾复方对成骨细胞（OB）、破骨细胞（OC）的药效异同，用MTT法测定OB增殖能力，骨陷窝计数定量评定OC骨吸收活性。

In order to compare differences of effects of complex prescription for reinforcing the kidney on osteoblast ( OB ) and osteoclast ( OC ), MTT method was used to determine proliferous capability of OB , bone lacuna count was used for quantitative assessment of absorbent activity of OC .