非变性聚丙烯酰胺凝胶电泳

经12%非变性聚丙烯酰胺凝胶电泳，溴化乙锭染色，紫外透射分析仪下观察并拍照。

After 12 % native polyacrylamide gel electrophoresis , ethidium bromide staining , observed and took photographs with ultra-violet transmission analysator .

PCR产物由非变性聚丙烯酰胺凝胶电泳分析。

The PCR products were analyzed by PAGE electrophoresis .

方法：选用不同pH的正极缓冲液和样品蛋白上样量，应用非变性聚丙烯酰胺凝胶电泳分离大鼠海马组织蛋白质。

Methods : Nondeture PAGE was used to separate proteins in different pH and loading quantity of protein sample .

方法用PCR扩增、非变性聚丙烯酰胺凝胶电泳分型；

Methods Two Y-STR loci were amplified with PCR . The products of PCR were analyzed with polyacrylamide gel electrophoresis .

方法：应用端粒重复扩增实验（telomeraserepeatamplificationprotocol，TRAP法）结合非变性聚丙烯酰胺凝胶电泳银染法测定35例大肠癌标本及正常黏膜端粒酶活性。

Methods : Combining telomerase repeat amplification protocol ( TRAP ) with PAGE silver staining , we detected the telomerase activity in cancer tissues and normal mucosa of 35 patients .

我们使用DMSfootprinting以及非变性聚丙烯酰胺凝胶电泳实验对G-quadruplex的形成进行了证明。

Using DMS footprinting and gel electrophoresis , we prove the formation of G-quadruplex .

采用不连续非变性聚丙烯酰胺凝胶电泳技术对42只岷县黑裘皮羊的血红蛋白（Hb）和转铁蛋白（Tf）基因座的多态性进行了研究。

The polymorphism of hemoglobin ( Hb ) and transferring ( Tf ) of 42 individuals of Minxian Black-fur sheep was detected by using polyacrylamide gel electrophoresis .

方法应用PCR和非变性聚丙烯酰胺凝胶电泳分型技术，并检验各基因座女性基因型频率分布是否符合HardyWeinberg平衡，计算法医学常用各种概率。

Methods The PCR products were detected by the polyacrylamide gel electrophoresis and silver staining method . Hardy-Weinberg equilibrium of females was tested and every forensically interested value was calculated .

方法采用聚合酶链反应（PCR）结合非变性聚丙烯酰胺凝胶电泳法检测了60例肾病综合征患儿及30例健康对照组Apo（a）（TTTTA）n基因多态性；

Methods PNTR polymorphism of the apo ( a ) gene from 60 children of primary nephritic syndrome and 30 controls were analyzed by polymerase chain reaction ( PCR ) denature polyacrylamide gel electrophoresis sliver stain .

方法采用苯酚/氯仿-二氧化硅-超滤离心方法提取东岭墓葬群殉人骨骼、牙齿古DNA，PCR扩增，非变性聚丙烯酰胺凝胶电泳（PAGE）分离和检测古DNA扩增片段。

Methods Ancient DNA fragments were extracted from the bones and teeth of sacrificial slaves with an improved method that combines phenol-chloroform extraction , silicon dioxide adsorption with ultrafiltration concentration . The polyacrylamide gel electrophoresis ( PAGE ) was used to detect PCR products .

我们的实验结果还发现，加热变性退火后非变性聚丙烯酰胺凝胶电泳可以提供一种衡量G-quadruplex在双链DNA中形成能力的简单方法。

Our results also showed that the heat denaturation / renaturation treatment followed by gel electrophoresis could provide a simple method to quantitatively access the ability of G-quadruplex formation in long double-stranded DNA .

本研究应用PCR-RFLP和DNA的非变性聚丙烯酰胺凝胶电泳银染法分析人的单核苷酸多态性及SNP与疾病的关联性的工作中，取得了比较满意的效果。

In this study , PCR-RFLP and DNA non-denaturing polyacrylamide gel electrophoresis by silver staining are used to analyze human single nucleotide polymorphisms and the relevance between SNP and disease , we have got satisfactory results . 4 .

取切割SD大鼠海马伞后14d及正常海马组织的匀浆进行非变性聚丙烯酰胺凝胶电泳（Native-PAGE）。

The hippocampi of SD rats on the 14th day after fimbria transection and the normal ones were extracted for Native-PAGE . ( 2 ) fimbria / fornix transection and ovariectomized group ;

对UIM-281所产粗酶液进行非变性聚丙烯酰胺凝胶电泳（Native-PAGE），活性染色结果表明该漆酶无同工酶。

The result of Native-PAGE for laccase crude extract showed that there is no isoenzyme of laccase existing in the UIM-281 .

PCR一SSCP分析时，凝胶的交联度对带型的清晰程度有很大的影响，非变性聚丙烯酰胺凝胶电泳图谱显示：以α乙酸萘酯为底物染色，2个东亚飞蝗种群谱带差别不明显。

PCR-SSCP is the analysis method for denatured PCR produce in non-denaturing polyacrylamide gel electrophoresis . No distinct difference was detected in banding patterns of general esterases between the two populations , when α - naphthyl acetate was used as substrate in non-denaturing polyacrylamide gel electrophoresis ( PAGE ) .

高粱微卫星非变性聚丙烯酰胺凝胶电泳条件的优化

Optimal of SSR-PCR Polyacrylamide Gel Electrophoresis Condition in Sorghum

由此可见，用6%非变性聚丙烯酰胺凝胶电泳分析的结果更接近田间鉴定结果。

It clearly showed that the results of6 % non-denaturing polyacrylamide gel electrophoresis much more close to the results of field planting test .

非变性聚丙烯酰胺凝胶电泳图谱显示：以α乙酸萘酯为底物染色，2个东亚飞蝗种群谱带差别不明显。

No distinct difference was detected in banding patterns of general esterases between the two populations , when α - naphthyl acetate was used as substrate in non-denaturing polyacrylamide gel electrophoresis ( PAGE ) .

分别用SDS-聚丙烯酰胺凝胶电泳法（SDS-PAGE）及非变性孔径梯度聚丙烯酰胺凝胶电泳法测定其分子质量，用聚丙烯酰胺管状等电聚焦电泳法测其等电点。

Its molecular mass was measured by SDS - polyacrylamide gel electrophoresis ( SDS-PAGE ) and native pore-gradient polyacrylamide gel electrophoresis respectively . Its isoelectric point ( pI ) was determined by isoelectric focusing tube electrophoresis .

用非变性（中性）聚丙烯酰胺凝胶电泳检测微卫星的PCR扩增产物。

The PCR amplified products of microsatellites were detected by non-denatured ( neutral ) polyacrylamide gel electrophoresis .