凝胶成像系统

本实验对该体系的条件进行了优化筛选。PCR扩增产物用凝胶成像系统及Quantityone定量检测，并通过多元回归曲线得到各组分信噪比的最大值。

PCR components in 16S rDNA amplification of fecal flora was optimized in the experiment , and PCR product was visualized by gel imaging system and quantified by using Quantity One Software , and obtained the best value from multi-regression curve .

结果均可通过电泳在凝胶成像系统上进行观察。

The results can be observed by electrophoresis gel imaging system .

PCR扩增产物可-20℃冻存供以后分析使用凝胶成像系统拍照。

PCR products can be - 20 ℃ frozen for later analysis using gel imaging systems camera .

运用凝胶成像系统中的定量软件Quantityone对各电泳图上的各条带进行定量分析，将图形结果转换为数据结果。

Use a quantitative gel imaging software Quantity One to quantify each band in the electrophoresis map and then convert the graphical results into digital results . 4 .

纯化蛋白在SDS-PAGE上呈现单一条带，凝胶成像系统软件分析纯化蛋白的纯度大于90%。

SDS-PAGE analysis showed that the target protein was highly purified and the purity was up to 90 percent .

方法应用半定量RT-PCR两步法和凝胶成像系统定量软件分析，分别对20株来自不同基因型的LDH进行基因表达水平差异的评价和比较。

Methods A semi-quantitative RT-PCR two-step method was developed to determine LDH gene against the housekeeping gene recA . Gene expression level of LDH was assayed by gel documentation system and QUANTITY ONES software .

方法以β-actin为内对照，用RT-PCR方法，对上述5种趋化性细胞因子进行30个循环PCR扩增，用凝胶成像系统观察扩增结果，并对各电泳带进行积分光密度扫描分析。

Methods Using β - actin as internal control , the mRNA of the chemokines listed above were amplified for 30 cycles with RT - PCR . The amplified products were observed by agarose electrophoresis , and each band was analyzed with integrated optical density .

凝胶成像系统对核酸扩增产物的定量检测

Quantitative detection of gel documentation systems on nucleic acid amplification products

计算机凝胶成像系统在生物工程研究中的应用

Application of Computerized Gel Imaging System in Research of Bioengineering

矩阵拆分方式在混合血清法核酸扩增技术中的应用目的：建立一种PCR-凝胶成像系统定量分析核酸扩增产物的方法。

Study on the Reduction of the Test Times by the Pooling of Serum Strategy of Splitting Matrix in Using Nucleic Acid Amplification Technology Objective : To establish polymerase chain reaction - gel documentation system ( PCR-GDS ) technology for detecting nucleic acid amplification products .

目的探讨利用凝胶成像分析系统进行DNA定量检测的DNA含量的范围。

Objective To investigate the applicaple scopes of DNA con-tent detected by gel imaging analysis system .

PCR结果经1.2%琼脂糖电泳并于凝胶成像分析系统下进行半定量分析。

PCR products were resolved in1.2 % agarose gel and were semi-quantity analyzed .

对于PCR凝胶成像分析系统来说，只有先对PCR凝胶图像进行正确的分析和提取，才能正确的对PCR产物进行定性、定量分析。

To the system of PCR gel image , correct qualitative and quantitative to PCR gel image are obtained only after correcting analysis and extraction for it .

GDS7600凝胶成像分析系统对蛋白质凝胶电泳板进行了成像和分析.然后再将图像、图表通过计算机处理插入到word文档中并编辑为word文档的内容。

The figures and tables from image acquisition and analysis system of GDS-7600 on protein gels , which have been edited with computer , can be inserted into Word document and become contents of Word .

将曝光后X-ray照片用UVP凝胶成像分析系统进行扫描，计算各基因蛋白条带与内参GAPDH蛋白条带的光密度比值，推算各凋亡因子表达的变化趋势。

Scan the exposure X-ray images with UVP gel imaging analytic system to calculate the respective optical density ratio of bands represent objective proteins to band of GAPDH . Then we can get the variation tendency of each proteins . 5 .

凝胶成像分析系统测试结果的计算机处理

Computer Processing of Testing Results from Image Acquisition and Analysis System

PCR扩增仪、电泳凝胶成像自动分析系统、电泳仪等。

Automatic analysis system of electrophoresis gel imaging ; electrophoresis apparatus , etc.

通过琼脂糖凝胶电泳和凝胶成像系统对多重PCR的扩增结果进行分析。

The PCR amplification productions were analyzed by Agarose Gel Electrophoresis in the Gel Imaging System .

PCR扩增结果经琼脂糖凝胶电泳以及凝胶成像系统分析后，并经测序进一步确证。

The results of PCR amplification were analyzed by Agarose Gel Electrophoresis , Gel Doc XR system and the results of PCR amplification were also confirmed by sequencing .

在PCR凝胶成像及定量分析系统中，为了正确检测出PCR产物，以便于对被检测者的病理状况作出准确的分析和处理，介绍了一种多聚酶链反应（PCR）成像信息的自动提取方法。

In the quantitative analysis of PCR gelation images system , in order to detect PCR correctly and make more accurate diagnosis of pathological situation of the person detected , a method to extract information automatically from PCR images is presented in this paper .

取浓度为62.50mg/ml试验组肿瘤细胞，提取DNA，经1%的琼脂糖凝胶电泳后，在凝胶成像系统下观察凋亡细胞形成的DNA条带。

48h later , do the experiment following . Extract DNA of tumor cells of experimental group ( 62.50mg / ml ) . After 1 % agar gel electrophoresis , observe characteristic DNA ladder due to apoptosis .