应用分子生物学

目的：尝试应用分子生物学软件研究DNA聚合酶β基因突变后，其编码蛋白、酶结构功能的改变。

Aim : A trial study of application of molecular biology software in the structural change of encoded protein and enzymes of mutated DNA polymerase β .

目的应用分子生物学方法判定经家系分析初步认定为X连锁遗传的高度近视家系的遗传方式。

Objective Using molecular biological method to determine the inherited modal of a family which was considered as X-linked high myopia by pedigree analysis .

结论：1.应用分子生物学方法可以成功地克隆大鼠GAP-43基因并构建以腺相关

Conclusion 1.Successful construction of the recombinant adeno - associated virus

很好地证明了应用分子生物学的PCR-DGGE技术可以揭示土壤生态系统中更全面的微生物信息。

All these results well demonstrated that application of PCR-DGGE technology can reveal more comprehensive information on microorganisms in soil ecosystem .

40d后处死大鼠，应用分子生物学、免疫组化等方法对AD鼠脑内细胞色素C蛋白质表达、白介素-1β转化酶mRNA、B细胞淋巴瘤mRNA等方面进行研究。

The rats were killed 40 days later to observe cytochrome C protein expression , IL-1 β converting enzyme ( ICE ) mRNA , Bcl2 mRNA in brain of AD rats with molecular biology and immunohistochemical assay .

应用分子生物学方法检测32例膀胱移行上皮癌中P53、K－ras、HPV基因，发现有34.4%的膀胱癌P53基因突变，21.9%膀胱癌K－ras基因突变，6.3%膀胱癌HPV阳性。

Cases of transitional cell carcinoma of bladder were detected for P53 , K-ras genes and HPV using molecular biologic techniques . P53 mutation was observed in 34.4 % , K-ras mutation in 21.9 % and HPV-positive in 6.3 % .

分别应用分子生物学RTPCR及免疫组织化学技术，探讨各组大鼠垂体钠泵α1、α2及α3亚单位mRNA及蛋白水平基因表达的改变。

Six weeks later , all the rats were killed and sodium pump α 1 , α 2 , and α 3 subunit were detected in the pituitary with RT PCR method and immunohistochemical assay at mRNA and protein levels , respectively .

方法：应用分子生物学研究方法，对HLA－DRB进行序列特异性引物的PCR扩增，鉴定了81例IDDM患者和84例正常对照汉族人群的DRB基因多态性。

Method : We Applied molecular biological technique to do the specula amplification on HLA-DRB using PCR . and identified the Dan gene polymorphism in 81 patients with IDDM and that of 84 normal control from Han Nationality .

本研究以高氧致早产鼠CLD模型为对象，应用分子生物学等检测技术，观察肺形态的动态演变过程；研究肺细胞的凋亡及其发生机制；

The aims of the study were at investigating dynamic pulmonary morphology change and at studying weather there are increased apoptosis and increased or decreased proliferation of lung cells as well as interstitial fibrosis and their pathogenic mechanisms in hyperoxia-induced premature rats .

应用分子生物学核酸杂交技术，以人乳头瘤病毒HPV6及HPV11型混合探针对食管ECA109鳞癌细胞株进行DNA斑点杂交。检测结果为阳性。

Human esophageal squamous cancer cell line ( ECa109 ) was analyzed by dot-blot hybridization to demonstrate the presence of human papillomavirus ( HPV ) infection , using a mixed DNA probe containing HPV types 6 and 11 . The result was HPV-DNA-positive .

应用分子生物学技术探讨慢性肺疾病和呼吸窘迫综合征的炎症反应机制

Inflammatory reaction in chronic lung disease and neonatal respiratory disease syndrom

应用分子生物学技术研究小鼠急、慢性肝损伤

Studies on Acute and Chronic Liver Injury in Mice by Molecular Biology Techniques

为今后临床应用分子生物学手段或基因治疗肿瘤提供资料。

Our research may be helpful to gene treatment of glioma by molecular biology methods .

应用分子生物学的研究方法可以快速准确地获得微生物多样性信息。

The application of molecular biology methods can provide a fast and accurate access to microbial diversity .

应用分子生物学方法诱导植物雄性不育：生物学基础和前景

The application of molecular method to the induction of male sterility in higher plants biological bases and Prospects

在过去的20年里，人们应用分子生物学技术来研制更为有效的结核病疫苗，新型候选疫苗大量涌现。

In the past two decades , new molecular technologies were used to develop a novel more effective vaccine .

这些菌属是潜在的标记物，应用分子生物学的方法检测这些菌属可作为细菌性阴道病临床诊断的靶标应用。

These genera are potentially excellent markers and could be used as targets for clinical BV diagnosis by molecular approaches .

同时表明，应用分子生物学手段来进行鲸肉及其制品的种类鉴定是可行和有效的。

The present study proves the feasibility and efficiency of using molecular techniques for species identification of whale meats or related products .

近年来，研究者做了大量工作，试图应用分子生物学方法鉴定医学重要真菌弥补传统方法的不足。

A lot of study based on molecular biology has been performed to establish new approach for the diagnosis and identification of medically important fungal infections .

应用分子生物学技术和免疫学技术进行病原学检测是诊断的金标准，但目前仍很薄弱，有待全面开展。

The pathogen detection with molecular biology techniques and immunological techniques is the gold standard for diagnosis , but it is still weak and is yet to be fully operational .

目的：探讨应用分子生物学技术对Down综合征进行基因诊断的可行性，建立一种快速、简便、准确的Down综合征的基因诊断方法。

Objective : Exploring the possibility of gene diagnosis for Down syndrome by means of molecular biological techniques and developing a rapid , simple and accurate gene diagnosis technique for Down syndrome .

自上个世纪80年代以来，应用分子生物学技术进行家族性糖尿病遗传学研究发现了一系列与糖尿病相关联的单基因，即胰岛素基因和胰岛素受体基因等。

In 1980s , molecula biology technology had been applied to familial diabetic genetics , a suit of single genes about diabetes was found : insulin gene , insulin receptor gene and so on .

应用分子生物学、分子遗传学等方法研究作物抗逆机制，找出抗逆基因，并通过基因工程手段培育优良的抗逆新品种是现代农业研究的重要内容。

It is an important field of modern agriculture to study the mechanism of stress-tolerance , obtain related genes in plant and cultivate tress-tolerant crops through molecular biology , molecular genetics methods and so on .

本文着重阐述了应用分子生物学技术在梨形虫基因组及染色体特点、致病的分子机制、抗感染免疫机制、基因工程疫苗研制方面的研究以及梨形虫病分子生物学诊断方面的应用。

This paper will focus on several aspects of piroplasmosis studied by molecular biology techniques , such as the characterization of genome and chromosomes , molecular pathogenesis , host immune response to infection , gene engineering vaccine and diagnosis by molecular biology techniques .

目的在获得含编码变形链球菌表面蛋白唾液粘附区片段基因的转基因番茄原代种子的基础上，应用分子生物学的方法检测外源基因在转基因植株中的表达。

Objective To analyze the expression of foreign gene in the filial generation of the transgenic plants on the base of the original transgenic tomatoes seeds carrying the gene encoding saliva-binding region ( SBR ) in PAc of Streptococcus mutans ( S.mutans ) gained .

目的：探讨人参皂甙对老龄大鼠内侧隔核TrkBmRNA表达的影响，为老年记忆减退性疾病的发病机制及人参皂甙的临床应用提供分子生物学基础。

Objective : To explore the effects of ginsenoside on TrkB mRNA expression in medial septal nucleus of aged rats and provide molecular basis for pathogenesis of memory failure .

应用PCR-DGGE分子生物学方法对东江水源工程沿途水样中的微生物群落结构的动态变化以及种群多样性进行了研究。

The dynamic metamorphose , composition structure and diversity of microbial community in the raw water along the Dongjiang River were studied by molecular biology PCR-DGGE assessment .

实时荧光PCR技术具有高灵敏度、高特异性、无需PCR反应后处理步骤等优点，近年来被广泛应用于分子生物学研究领域，尤其在临床检验方面，该技术提供了有力的支持。

Recently , real-time polymerase chain reaction ( RT-PCR ) has been widely used in the research of molecular biology , especially in clinic diagnosis , because of its high specificity , sensitivity and without any further processing of the samples and opening the test tubes .

目前MT-3T3细胞已经应用于分子生物学和分子病理学的研究。

Now the MT-3T3 cell has been used in the research of molecular biology and molecular pathology .

在治疗学方面，早已经有很多目的性很强的应用分子-生物学方法治疗缺血的例子。

Therapeutically , there are already intriguing examples of targeted molecular-biological ap-proaches to treatment of ischemia .