显微切割

通过激光显微切割技术和微量DNA提取技术，共获得了13份绵羊肝脏组织不同时期、不同细胞的基因组DNA样品。

By Laser microdissection and micro DNA extraction , we got 13 cell samples of different periods . 5 .

结果表明，染色体显微切割和简并PCR技术相结合，可以有效地制备鱼类DNA探针。

It was obvious that the combination of chromosome microdissection and DOP PCR could efficiently produce DNA probes for fish .

人染色体3p14-24区域的显微切割及其DNA的分离和鉴定

Characterization of Microdissected Human Chromosome Region 3 p 14 - 24

用显微切割-PCR检测石蜡标本微小病灶DNA异常

Microdissection and Polymerase Chain Reaction Amplification of Genomic DNA from Minute Lesions in Paraffin-embedded Tissue Sections

鼻咽癌组织的显微切割及其RNA线性扩增

Microdissection and RNA Line-amplification of Nasopharyngeal Carcinoma Tissue

PCR方法比免疫组化能明显提高EBV检出率；利用显微切割技术能很好地提高目的细胞的富集度，提高检出率和准确性。

Microdissection is very helpful to improve the detection rate and accuracy ;

免疫组织化学-激光显微切割-聚合酶链反应技术在胃癌石蜡切片中检测p53突变的应用

Application of immunohistochemistry-laser microdissection-PCR technique in detecting p53 gene mutation in paraffin sections of gastric cancer

本文用显微切割，PCR技术和染色体绘画技术，构建了人类第七号染色体特异性探针池，并完成了一个7号染色体结构异常患者的家系分析。

We constructed a chromosomal specific probe pool from human chromosome No. 7 using the techniques of chromosomal microdissection , PCR and chromo -

激光捕获显微切割（LaserCaptureMicrodissection，LCM）与基因芯片是后基因组时代新出现的两种技术。

Laser capture microdissection ( LCM ) and microarray are two new immerging techniques in post-genome era .

联合应用激光显微切割和RT-PCR分析膀胱移行细胞癌中SURVIVINMRNA表达及意义

Expression and significance of survivin mRNA measured by laser cut microscopy combining RT-PRC in transitional cell carcinoma of the bladder

显微切割进一步证实B19病毒DNA存在于肿瘤细胞内。

Furthermore , laser captured microdissection confirmed that the B19 DNA was located in the neoplastic cells of colorectal adenocarcinoma .

用显微切割技术定点检测诱发大鼠肺癌发生发展各阶段p53、K-ras基因突变与表达

Microdissection and PCR-SSCP detected mutation and expression of p53 and K-ras gene in carcinogenesis and development of induced rat lung carcinoma

显微切割技术分离微量肿瘤组织制备DNA进行聚合酶链反应扩增；变性高效液相色谱技术（DHPLC）和DNA测序技术检测内源性p53基因突变的分布和类型。

Microdissection , PCR , and denaturation high performance liquid chromatography ( DHPLC ), and DNA sequencing were used to detect the mutation of p53 gene .

激光捕获显微切割（LaserCaptureMicrodissection，LCM），是一项利用激光在显微镜下从组织切片中分离单一类型细胞群的技术。

Laser capture microdissection ( LCM ) is designed to isolate homogeneous populations of cells from histologic sections of complex tissues under direct microscopic visualization .

目的探讨激光捕获显微切割分离大鼠脑微血管和神经元联合功能分类表达谱cDNA芯片技术在脑缺血研究的应用。

Objective To develop a method to analyze functional grouping cDNA microarray gene expression in microvessels and neurons from rat brain using laser capture microdissection ( LCM ) .

方法：采用病理切片上病灶显微切割、P53基因突变的PCRSSCP分析及DNA测序测定3例患者前列腺癌根治切除术标本中癌灶和高分级PIN灶组织；

Methods : Point mutation of p53 gene in 7 lesions of prostate carcinoma and high grade PIN ( Prostatic intraepithelial neoplasia ) in 3 samples of radical prostatectomy was examined by PCR SSCP assay and DNA sequencing .

目的探索激光捕获显微切割技术（LaserCaptureMicrodissection，LCM）定位分离心房细胞，并对提取的微量RNA进行β-actin及GAPDH基因扩增。

Objective To use the laser capture microdissection ( LCM ) to isolate the pure atrial cells from heart . To extract tiny amount RNA and amplify the genes of β - actin and GAPDH .

结论利用本室创建的显微切割-CDNAPCR-SSH法成功构建胃异型增生组织cDNA消减文库，初步筛选部分基因，为寻找胃癌发生相关基因提供重要线索。

Conclusions Subtracted cDNA libraries from gastric dysplasia are constructed using combination of microdissection-cDNA PCR and SSH setup in our laboratory . Some fragments have been screened and verified to help to search for novel associated genes with gastric carcinogenesis .

新的蛋白质组学技术如激光捕获显微切割（LCM），串联质谱、二维液相分离-质谱鉴定等加速了乳腺癌研究的进展。

The research of breast cancer will be developed more quickly by means of newer proteomic technologies such as laser capture microdissect ( LCM ), tandem MS and 2D-LC-ESI-MS , etc.

激光捕获显微切割（LCM）是近年来发展起来的从杂乱的多细胞组织中分离特定细胞群的新技术，分离的细胞群还可应用于后续的DNA、RNA和蛋白质的分析。

Laser Capture Microdissection ( LCM ) was a novel technique recently developed to isolate pure population of specific cells from miscellaneous tissues . The captured cell population can be used in the subsequent assay for DNA , RNA and protein .

方法经显微切割技术获取前列腺癌及PIN各10例患者DNA，采用聚合酶链反应（PCR）及微卫星多态性技术，对6号染色体上的20个微卫星标志位点LOH进行检测。

Methods Pure DNA was obtained from prostate neoplasms and normal tissues after tissue microdissection . LOH of chromosome 6 was detected by PCR based microsatellite polymorphism analysis technique using 20 pairs of microsatellite primers in 10 prostate carcinoma cases and 10 high grade PIN cases .

方法应用显微切割制备的7号特异性全染色体探针和7q亚端粒（7q36→qter）探针，与病例的中期分裂相进行荧光原位杂交（FluorescenceinSituHybridization，FISH）。

Methods Fluorescence in situ hybridization ( FISH ) technique was performed to analyze the case by using whole chromosome 7 painting probe and subterminal probe of 7q36 → qter which was generated by chromosome microdissection technique .

近年发展起来的激光捕获显微切割（LaserCaptureMicrodissection，LCM）技术可以在显微镜直视下快速、准确地获取所需的单一细胞亚群，甚至单个细胞，从而成功解决了组织中细胞异质性问题。

Laser capture microdissection ( LCM ) is a novel technique that can be applied to obtain pure targeted cell subgroup or even a single cell quickly and precisely under the microscope , thus the problem of tissue heterogeneity in molecular analysis can be tackled successfully .

本研究利用显微切割技术及PCR扩增技术，探寻棉花的着丝粒序列，主要研究结果如下：1.棉花染色体或染色体片段微分离及LA-PCR技术体系的建立。

In this study , we used microdissection and PCR technology , explore the cotton centromere . The main results were as follows : 1 . Establishment of chromosome or chromosome segment microdissection and LA-PCR technology in cotton .

初步进行了激光显微切割系统分离的杨树染色体的分子生物学研究。

Molecular biology of laser micro-dissectioned chromosome in Populus was studied .

运用激光捕获显微切割对肝细胞癌与肝内胆管癌蛋白质组的分析

Proteomic analysis of hepatocellular carcinoma and cholangiocarcinoma by laser capture microdissection

一种利用染色体显微切割和微克隆获得小麦基因探针的方法

A Method of Using Chromosome Microdissection to Obtain Gene Probe in Wheat

胶片吸附的激光显微切割技术及其在蛋白质组研究中的应用

Laser Capture Microdissection ( LCM ) with its Applications in Proteome Analysis

激光微束显微切割植物染色体的研究

Study on laser micro - dissection of chromosome in plants

应用激光显微切割技术检测单个结肠细胞的基因表达

Laser microdissection of a single cell from colon tissue for gene analysis