细胞化学技术

本文利用电子显微镜和酶细胞化学技术就三唑酮种衣剂包衣处理对小麦幼苗内酸性磷酸酶（APase）分布的影响进行了研究。

Effect of seed coating treatment with triadimefon on the distribution of Acid Phosphatase ( APase ) in wheat seedlings was investigated using the techniques of electron microscopy and cytochemistry .

利用草酸-焦锑酸钾电镜细胞化学技术观察缺血脑组织的超微结构改变。

Changes of ultrastructure in ischemia brain tissue were observed with oxalic-potassium pyroantimonate electron microscope cytochemistry technique .

免疫细胞化学技术检测P－糖蛋白在人膀胱癌MDR细胞亚株中的表达

Detection of P-glycoprotein expression with immunocytochemistry in adriamycin-resistant human bladder cancer cell line

方法改良MTT比色法、免疫组织细胞化学技术和WESTERNBLOTTING等方法。

Methods MTT assay , immunohistochemical method and Western blotting were adopted .

方法应用Northern印迹杂交和免疫细胞化学技术。

Methods Northern blot analysis and immunocytochemical stain were used on cultured rat pulmonary fibroblasts ( PFBs ) after FN treatment .

应用免疫细胞化学技术检测用药前后凋亡相关基因bcl-2、bax表达的变化。

The expression of apoptosis associated genes bcl-2 and bax was observed by immunocytochemical staining .

免疫荧光细胞化学技术及半定量分析心肌细胞促凋亡基因c-fos的蛋白表达。

Cfos protein in cardiomyocytes was detected using semiquantitative immunofluorescence cytochemistry technique .

方法：采用免疫细胞化学技术分析患儿骨髓单个核细胞bcl-2、bax蛋白的表达。

Method : The expression of bcl 2 and bax protein in normal and leukemic mononuclear marrow cells were measured by immunocytochemistry staining .

利用间接免疫荧光技术和免疫细胞化学技术检测细胞内凋亡相关蛋白Caspase-8、Caspase-3以及死亡底物聚ADP核糖聚合酶[poly（ADP-ribose）polymerase，PARP]的表达活性变化。

Expression of apoptosis-related proteins ( Caspase-3 , Caspase-8 and poly ADP-ribose polymerase ( PARP )) was determined by immunohistochemistry .

本文应用免疫细胞化学技术对新研究成功的结肠癌相关抗原LEA在光镜、电镜下进行了较详细的定位观察研究。

The distribution of LEA , a new colorectal carcinoma-associated antigen , was examined in details by immuno - light and immunoelectron microscopy .

采用免疫细胞化学技术对禾谷镰刀菌（Fusariumgraminearum）在侵染小麦穗部过程中产生的脱氧镰刀菌烯醇毒素（deoxynivalenol，DON）进行了定位分析。

Fusarium toxin deoxynivalenol ( DON ) was localized in wheat spikes during infection process of Fusarium graminearum by means of immunocytochemical labeling technique .

为了快速检测特异性染色体，采用引物原位标记和细胞化学技术，以7号，17号，X和Y染色体特异性寡核苷酸作引物，对外周血淋巴细胞分裂中期染色体和间期核进行定位研究。

In order to identify specific chromosomes rapidly , with specific primers for chromosome 7 , 17 , X and Y , both metaphase and interphase nuclei of peripheral blood lymphocytes were detected by primed in situ ( PRINS ) labeling technique .

方法：对冲击波暴露后不同时间组采用免疫细胞化学技术（ABC）结合图像分析和听性脑干反应测试技术进行观测。

To further study the mechanism of blast trauma causing deafness . Methods : After shock wave , different groups of guinea pigs were studied by ABC immunohistochemistry , image analysis techniques and auditory brainstem response .

应用电镜酸性磷酸酶（ACP）细胞化学技术显示大鼠脊髓腹角神经元的ACP酶活性分布，并进行了超高压电子显微镜观察。

Under high voltage electron microscope , we studied the distribution of ACPase activity in neurons of ventral horn in rats ' spinal cord by ACPase zymo cytochemical technique .

采用免疫荧光细胞化学技术、RT-PCR和基因测序法共同检测NSCs标志物Nestin的表达；

The expression of nestin gene , which is specific to NSCs in the CNS , was detected by immunofluorescence technique , reverse transcriptase polymerase chain reaction ( RT-PCR ) and gene sequencing .

存活不同时间后，用AChE酶细胞化学技术染色脑切片，用图象分析系统测算海马层AChE阳性神经元及纤维的体积密度。

After different time of survival , brain sections were stained with AChE enzymatic cytochemical technique and the volume density of AChE positive neurons and fibers in hippocampus and cerebral cortex were measured by an image processing system .

方法：应用AgNOR定量诊断和CEA、CK及VIM免疫细胞化学技术对56例浆膜腔积液进行对比研究。

Methods : We used AgNOR quantitative diagnosis and immunocytochemical techniques , including carcinoembryonic antigen ( CEA ), cytokeratin ( CK ) and vimentin ( VIM ) to determine the cells in serous fluid from 56 patients .

方法：应用原位杂交和免疫细胞化学技术，分别对人膀胱癌耐药细胞株（BIU87/ADM）及敏感细胞株（BIU87）中Bcl2基因及其产物的表达进行研究。

Methods : The expression of Bcl 2 gene in cell lines BIU 87 / ADM and BIU 87 was detected with in situ hybridization and immunohistochemistry .

用免疫细胞化学技术对大鼠脊髓背角内Fos阳性神经元和SP受体阳性神经元的分布及相互间关系进行了研究。

The present study was to investigate the distribution and relationship of c fos oncogene expressing protein product ( Fos ) positive neurons and substance P receptor ( SPR ) positive ones within the spinal dorsal horn of the rat by means of the immunocytochemistry .

以7例18至34周龄死亡人胎冠状动脉作材料，用免疫细胞化学技术ABC法显示NPY样神经纤维，用显微分光光度计扫描测定冠状动脉各分支NPY样神经纤维的含量。

The relative contents of NPY-Like nerve fibres in coronary artery branches were analyzed by scaning with a microspectrophotometer . The coronary artery branches come from seven cases of hu - man fetuses ranging from 18 to 34 weeks were stained by immunocytochemical ( ABC ) technique .

实验采用振荡分离和差速贴壁等方法进一步分离OPCs，通过免疫细胞化学技术对分离后的OPCs进行分析鉴定。

After the primary culture in vitro , the OPCs were further dissociated by the shaking process and differential adhesion . Then , the separated OPCs were also identified and analyzed by the immunocytochemical technique . 3 .

免疫细胞化学技术在转录因子激活研究中的应用

Ap plication of immunocytochemistry in the study of transcription factor activation

生活污水用于园林绿化的实验应用电镜细胞化学技术观察E。

Experiment of Applying Living Waste Water in Landscape Greening Living E.

免疫细胞化学技术检测不孕妇女血清抗精子抗体

The detection of serum antisperm antibody of infertile women by immunochemical assay

免疫细胞化学技术在胸腹水脱落细胞学诊断中的应用

Application of immunocytochemistry to cytological diagnosis in hydrothorax and ascites

试论免疫组织、细胞化学技术及其应用

The Techniques of Immune Tissue and Cytochemistry and Their Application

应用免疫细胞化学技术对培养细胞及其分化细胞进行鉴定。

Immunohistochemistry were used to identify the type of cells .

免疫细胞化学技术、克隆分析等方法评价其生理特性。

Immunocytochemical techniques and clonal analysis were used to assess cellular physiological characteristics .

利用电镜细胞化学技术对毛竹竹秆基本组织发育过程中过氧化物酶进行了细胞化学定位。

The Ultracytochemical localization of peroxidase during the ground tissue development in bamboo Phyllostachys edulis culm was studied with cytochemical technology .

结论：用免疫细胞化学技术检测细胞核蛋白时，最好选用甲醇作为固定剂。

Conclusions : The results indicate that examination of nucleoproteins in cells by immunocytochemistry using the fixative like formaldehyde needs detergent extraction .