花粉管通道

花粉管通道法转基因改良小麦品质的初步研究

Study on the improving of wheat quality by pollen tube pathway transgene

花粉管通道法介导的抗大麦黄矮病毒转基因小麦研究

Transgenic wheat resistant to Barley Yellow Dwarf Virus obtained via pollen tube pathway

外源DNA导入花粉管通道技术的发展和应用

Application and Development of Foreign DNA Introduction via the Pollen-tube Pathway

外源DNA通过花粉管通道法导入玉米的研究

Introduction of Exogenous DNA into Maize via Pollen Tube Pathway

烟草花粉管通道导入外源DNA研究初探

Primary Study on Exogenous DNA Introduction into Tobacco Via the Pollen-tube Pathway

为供体，宁春4号小麦为受体，采用花粉管通道法进行外源DNA导入。

As donor and wheat as recipient .

DNA浓度及注射时间对苹果花粉管通道法基因转化率的影响

Concentration of DNA and Time of Injection on Gene Transformation Efficiency via Pollen-tube Pathway in Apple

用花粉管通道法将bar基因导入水稻获得可遗传的转基因植株

Transgenic Rice Plant Obtained from Introduction of Herbicide Resistant Gene bar into Rice by Pollen-tube Pathway Method

利用花粉管通道技术创造棉花变异种质及其SSR标记分析

Variant germplasm obtained by pollen tube pathway technique and SSR analysis

还表明：利用花粉管通道途径来实现外源DNA直接导入大豆，进行大豆种质创新和品质改良也是可能的。

The results also indicate that it is possible to improve soybean quality by using the pollen tube passage to transfer foreign DNA into soybean .

利用花粉管通道技术，将Bt基因导入大豆品种。

Bt gene was introduced into soybeans by pollen tube path in this paper .

用花粉管通道法将慈姑蛋白酶抑制剂基因（API）导入水稻获得转基因植株

Transgenic Rice Plant Resulting from Introduction of Arrowhead Proteinase Inhibitor Gene ( API ) into Rice by the Pollen-tube Pathway Method

花粉管通道法转水稻Waxy基因

Transformation of Rice Waxy Gene Via Pollen-tube Pathway

本文利用花粉管通道在大豆自花受粉后，将野生大豆DNA导入栽培大豆，从而引起了后代性状的广泛变异。

This study reports that wild soybean DNA was introduced into cultivar soybean through pollen tube after soybean self-fertilizing , it led to wide variation in offspring characteristics .

利用花粉管通道法，用含抗除草剂基因bar及抗虫基因pin2的质粒pTW-α转化中籼HD357。

Via pollen - tube pathway the indica rice HD - 357 was transformed with plasmid pTW - α containing Bar gene and PIN ⅱ gene .

PINⅡ基因通过花粉管通道法转化水稻的研究

Studies on culture of insect - resistant rice by transforming rice with PIN ⅱ gene via the pollen - tube pathway

用花粉管通道法将野生大豆总DNA导入小麦，获得了转基因小麦，其后代的农艺性状有较大的变化。

The DNA of Glycine soja was introduced into wheat through the pollen tube pathway after self pollination . The T 2 generation population 's agronomic traits have greatly changed .

GUS与天花粉蛋白基因由花粉管通道导入小麦的研究

Introduction of GUS and TCS GNES into wheat via the pollen & tube pathway

采用花粉管通道法，在受体哈师5号授粉后，将经过辐照处理的供体DNA整体导入到哈师5号中，并对所得后代不断进行筛选和研究。

With the pollen-tube pathway , the exogenous DNA that had been treated with radiation was introduced into Hashi 5 after its pollination and its descendants were selected and researched unceasingly .

采用花粉管通道法遗传转化技术，将慈姑蛋白酶抑制剂基因（API）导入水稻品系9311中，获得了转基因植株。

Transgenic rice plants were obtained by transferring the arrowhead proteinase inhibitor gene ( API ) into rice line 9311 through the pollen-tube pathway method .

本研究以异9&3柱头外露棉为供体，将提取的基因组DNA通过花粉管通道法经微量注射导入受体抗虫棉707-1的胚囊内，在分离的群体中的田间筛选到柱头外露的转化植株。

Via pollen-tube pathway the genomic DNA of the open-bud cotton Yi 9-3 was introduced into the insect-resistant cotton 707-1 . The variant line with open-bud was obtained from their segregation generation .

授粉后72h，早期胚形成；④应用花粉管通道法对棉花导入外源DNA的适宜时期为授粉后18~48h。

The pollen tube pathway observation showed that the suitable time for introducing exogenous DNA was 18 hours to 48 hours after pollination .

利用花粉管通道法将叶片衰老抑制基因P（SAG12）-IPT导入普通小麦的研究

Studies on Introduction of Leaf Senescence-inhibition Gene P_ ( SAG12 ) - IPT into Common Wheat through Pollen-tube Pathway

高分子量麦谷蛋白亚基1B×14在花粉管通道法转基因小麦中的表达研究

Research on Expression of HMW-GS1B × 14 Gene in Transgenic Wheat Via Pia Pollen Tube Pathway

以甘蓝型油菜（3529&5）为受体，诸葛菜为供体，用花粉管通道法，在自花受粉24小时后，导入外源总DNA。

Exogenous total DNA extracted and purified from the donor ( Orychophragmus violaceas ) was injected into the ovary of B. napus ( 3529-5 ) through the pollen tube channel after 24 hr artificial pollination .

本试验选用菰（Zizania）作供体，栽培水稻品种为受体，采用花粉管通道法进行DNA转移的研究。

DNA transfer was studied using pollen tube passage method with wild rice ( Zizania ) as donor and rice ( O. Sativa ) as recipient .

利用花粉管通道法和浸种及苗期浇灌法，将供体大豆DNA直接导入受体水稻，获得两种水稻变异株系。

Two groups of rice variant lines increased their protein contents were obtained by introducing exogenous DNA from soybean into rice with soaking seeds , moreover irrigating seedlings , and through the pollen tube pathway after self pollination .

总DNA通过花粉管通道导入普通小麦宁春4号，变异品系的光合特性及农艺性状发生变化：部分变异品系的旗叶功能期以及叶绿素含量改变，光合速率加快；

Was transferred into common wheat ( Ningchun 4 ) . Consequently , several variant strains were changed in photosynthetic and agronomic traits : the period of photosynthetic function of flag leaf and the chlorophyll contents were altered ;

本试验利用基因枪法和花粉管通道法两种植物遗传转化技术，将叶片衰老抑制基因P（SAG12）-IPT导入有早衰现象的小麦品系9848，获得转基因植株。

In this study , transgenic wheat plants of 9848 with P_ ( SAG12 ) - IPT gene were obtained by micro-projectile bombardment and pollen tube pathway .

经过我们15年的悉心研究，通过花粉管通道法将高粱DNA导入小麦，结果来自高粱的外源基因不但整合到小麦染色体，而且得到了表达。

After 15 years studied , it is indicating that the gene from sorghum not only integrated into wheat but also expressed by introduced exotic DNA from sorghum ( 6A / 115 ) into normal wheat through the pollen tube pathway .