蓝白斑筛选

试验中通过蓝白斑筛选及PCR验证得到重组杆粒。

The recombinant bacmids were screened by blue-white selection and verified by PCR .

二者连接后，转化入DH5α感受态细胞（大肠杆菌）中。用蓝白斑筛选法识别PCR产物是否与pUC19载体连接。

After ligation , they were transformed into DH5 α competent cells ( Escherichia coli . )

经测定包装滴度为5.23×10~6pfu／ml，蓝白斑筛选重组率为96.8%，表明已成功构建血清1型RA基因文库。

The results showed that the packaging titer was 5.23 × 10 ~ 6 pfu / ml and recombinant rate was 96.8 % . It is concluded that the constructed genomic library can be used to screen target clones .

经PCR鉴定得到重组病毒，经过蓝、白斑筛选得到纯化的重组病毒；

The recombinant virus was got through PCR identification and the purified recombinant virus was got by blue and white plaque assay .

经蓝、白斑筛选，共得到了142个阳性克隆。

The blue , white spot screening , a total of 142 positive clones were obtained .

通过连接、转化构建了基因工程菌，通过蓝白斑实验筛选阳性菌株。

Gene engineering bacteria were constructed by technology of linkage and transformation and positive bacterial strains were picked up by blue-white spotting test .

ColiDH5α，用蓝/白斑试验筛选阳性克隆，抽提质粒进行酶切鉴定，再行序列分析。

Coli DH5 α was transformed with recombinants and screened with blue / white blot test for positive clones from which plasmids were then extracted , the recombinants were identified by restricted analysis and sequencing .

蓝白斑α互补筛选出含有外源插入片段的阳性克隆，结果正向消减杂交产物得到286个克隆，反向消减杂交产物得到262个克隆；

Positive clones containing exogenous insert were screened by blue white alpha complementation , and 286 and 262 clones were obtained with forward and backward subtractive hybridization products respectively ;