辅基

在体外O·－2损伤CAT的过程中，对CAT辅基Fe（Ⅲ）的ESR波谱进行了分析研究。

We studied the ESR spectra of the prosthetic group Fe (ⅲ) in injuring CAT with O · - 2 in vitro .

血红素是血红蛋白的辅基，有极其广泛和重要的应用。

Heme , the prosthetic group of the hemoglobin , has been used widely and importantly .

在体系中，共聚物与脱辅基细胞色素c之间的相互作用和共聚物与蛋白质自身的疏水聚集之间存在竞争。

A competition exists between the interaction of the copolymer with apo cyt c and the self-aggregation of PTMA or apo cyt c alone .

以易得的对映体纯的亚砜为手性辅基诱导的不对称共轭加成反应为关键反应，对映选择性地合成了倍半萜（S）和（R）-Cururphenol。

The key step in this synthesis is asymmetric conjugate addition using the readily available optically pure sulfoxide as chiral auxiliary ;

O2~（·-）损伤过氧化氢酶过程中辅基Fe（Ⅲ）的ESR波谱

ESR Spectra of Prosthetic Group Fe (ⅲ) in Injuring Catalase with O · - 2

CYTC分子由肽链将c型血红素辅基紧紧包裹在其中，分子近似为球形。

The heme is closely surrounded by the protein matrix , which brings Cyt c a globular shape .

在酸性和中性溶液中磺化聚苯乙烯和脱辅基细胞色素c发生相互作用形成复合物粒子，其尺寸与两者的浓度比有关。

In acidic and neutral pH , SPS interacts with apo cyt c forming complex particles , whose size depends on the relative amount of apo cyt c and sulfonated polystyrene .

在此基础上，我用动态光散射、荧光光谱和原子力显微镜方法对磺化聚苯乙烯和脱辅基细胞色素c的相互作用进行了进一步地表征。

I further study the interaction of SPS particles and apo cyt c using a combination of techniques , i.e. , dynamic light scattering , steady-state fluorescence , and atom force microscopy .

说明：辅基Fe（Ⅲ）的微观环境发生了变化，提示：这些变化可能是O·－2造成CAT活性改变的主要分子机制。

The results show that the Fe (ⅲ) microenvironment had changed , which may be a main molecular mechanism of CAT activity change in injuring with O · - 2 .

同时，与CYTC或Mb相比，cytb5分子中辅基heme有着更大的溶液暴露程度。

At the same time , the heme of cyt b5 is relatively more exposed to solvent in comparison with cyt c or Mb .

其分子由一个酸性的辅基蛋白和一个发色团结合而成，发色团是LDM的主要活性部分，起着直接断裂DNA的作用，而辅基蛋白的主要作用是保护发色团。

It consists of a chromophore and an apoprotein , and the former has the activity to attack DNA , whereas the latter plays the role in protecting chromophore .

新辅基吡咯喹啉醌（PQQ）生物合成基因研究进展

Progress in the Biosynthesis Gene of a New Prosthetic Group Pyrroloquinoline Quinone

依赖新辅基PQQ甲醇脱氢酶的研究

Studies on a New Prosthetic Group PQQ Dependent Methanol Dehydrogenase

蛇毒L-氨基酸氧化酶大都含有两个相同非共价结合的辅基FAD或者FMN。

Snake venom LAOs are usually dimeric flavoprotein , which contain non-covalently bound FAD or FMN as cofactor .

通过氨基酸替换提高GFP脱辅基蛋白在高温，如37℃条件下的正确折叠，以及改变GFP光谱特性。

The specific amino acids were substituted to improve the apoprotein folding at high temperature , such as 37 ℃, and change the spectral properties of GFP .

采用SDSpage凝胶电泳及电转移方法，将增溶的鼠肝线粒体胆碱脱氢酶进一步纯化，且去掉增溶线粒体胆碱脱氢酶（CDH）中所包含的大部分磷脂、去垢剂、辅基FAD等。

Solubilized rat liver mitochondrial choline dehydrogenase was further purified by the SDS PAGE and electroblotting methods . Both the lipids and detergents etc. were removed during the process of purification .

首先在体外进行了这四种蛋白酶与脱辅基蛋白CpcA之间的复合物实验。

This paper designed protein complex experiment in vitro of four proteases and apoprotein CpcA .

在中性和碱性溶液中，共聚物和蛋白质之间的相互作用破坏了PTMA和脱辅基细胞色素c自身的疏水聚集，形成了新的聚集体。

The interaction of the copolymer with apo cyt c at neutral and alkali pH destroys the hydrophobic aggregation of PTMA or apo cyt c and forms new complex particles .

在本论文中，用磺化聚苯乙烯、马来酸&烯烃交替共聚物研究了静电、疏水作用和氧键对细胞色素c和脱辅基细胞色素c结构的影响。

In this thesis , I investigated the influence of the electrostatic and hydrophobic interactions and hydrogen bonding on the structure of apo cytochrome c ( apo cyt c ) and cytochrome c ( cyt c ) with sulfonated polystyrene and the alternating copolymers of maleic acid and alkene .

脱辅基酶与Co（Ⅱ）离子结合，酶的主链构象恢复至与天然酶几近相同。可认为这是促使酶复活的内在因素。

It also worth noting that the conformation of the Co (ⅱ) - substituted enzyme is similar to the native enzyme , and this may be the reason for restoring of the enzymatic activity of metal-free apoenzyme upon the addition of Co (ⅱ) ion .

单链抗体与lidamycin辅基蛋白融合蛋白工程菌的高密度发酵及其免疫活性

High cell-density fermentation of the engineered strain and immunological activity of the fusion protein composed of single-chain Fv antibody and lidamycin apoprotein

固氮酶Fe-Mo辅基模拟物Fe-Mo-S原子簇成键轨道的波函数图形分析

The Map Analysis of Bonding orbital Wave Function of Fe-Mo-S Cluster the Analogue of the Nitrogenase Fe-Mo-Cofactor

研究了7T超导稳态强磁场在不同处理时间条件下对小麦幼苗的抗氧化系统（SOD、CAT活性和MDA含量）的影响，并对不同金属辅基SOD酶的诱导表达模式进行了探讨。

The effects of superconducting SMFs up to 7-tesla on antioxidation system of wheat seedling ( SOD , CAT activity and MDA content ) and the expression pattern of SOD isoenzyme with different metal prosthetic groups were investigated .

吡啶血色素光谱显示了酶分子以原卟啉Ⅸ（protohemeⅨ）作为辅基。

The pyridine hemochrome spectrum indicated the presence of protoheme ⅸ as the prosthetic group .

在前报道中已经鉴定出绿脓杆菌去辅基天青蛋白突变体M121L可以多种构象共存。

In a previous report , the conformation heterogeneity of apoazurin mutant M121L had been identified .

由于金属辅基的不同，它可分为四类，分别为Cu/Zn-SOD、Mn-SOD、Fe-SOD和Ni-SOD。

According to the type of metal in the active site of the enzymes , SOD has been classified into four types : Cu / Zn-SOD , Mn-SOD , Fe-SOD and Ni-SOD .

指出HRP可在固体电极上进行直接电子传递，该电极反应不是酶中二硫键的还原，而是血红素辅基中心金属离子的氧化态转变。

It is shown that the direct electron transfer of the enzyme HRP on the solid electrodes can be carried out and the electrode reaction is the oxidation-state transformation of the central metal ion in prosthetic group heme rather than the reduction of disulfide group in HRP molecule .

小分子靶向肽与力达霉素辅基蛋白偶联方法研究

Study on the Method of Conjugating RGD Peptide to Lidamycin Apoprotein

具有加卤过氧化物酶活性含卟啉辅基的抗体酶研究

Studies of Chloroperoxidase-Like Abzyme Containing Prosthetic Group of Porphyrin

藻胆蛋白脱辅基蛋白对其抗氧化活性的影响

Effect of apoprotein on antioxidant activity of phycobiliproteins