不完全佐剂

然后每隔14天后再用弗氏不完全佐剂与之混合，共免疫2次，末次加强免疫以100μg／只尾静脉注射，3d后取小鼠脾细胞与骨髓瘤细胞Sp2／0-Ag-14进行融合。

Fourteen days later , the same antigen emulsified in in freund 's incomplete adjuvant were intraperitoneally . and three days after final immunization , Splenocytes from the immunized mice were fused with Sp2 / 0-Ag-14 myeloma cells . Keep it in the bloom of proliferation before cell fusion .

方法运用自提的猪囊虫囊液与福氏不完全佐剂加干扰素混合免疫Balb/c小鼠，取小鼠脾淋巴细胞与SP2/0骨髓瘤细胞融合，反复筛选及克隆化；

Methods Spleen cells of Balb / c mouse immunized subcutaneously with cysticercus fluid ( mixture of Freund 's incomplete adjuvant and interferon ) were fused with myeloma cells SP2 / 0 , and the resulting hybridoma cells were selected and cloned .

PAGE电泳后，将表达的CP蛋白切胶回收，研磨成粉后加入等体积的福氏不完全佐剂，乳化制备成抗原。

After SDS-PAGE , the recovery of CP added the same volume of incomplete Freund Adjuvant emulsified completely , injected rabbits by subcutaneous injection .

用不同培养基制备的精制破伤风类毒素，加入福氏不完全佐剂免疫豚鼠，测血清中抗体效价。

Guinea pigs were immunized with Tetanus toxoid prepared with different medium .

但随后的注射则以使用不完全佐剂为宜。

But incomplete adjuvant is adequate for subsequent injections .

方法：采用弗氏完全／不完全佐剂，经皮下多点注射免疫；

Methods : complete / imcomplele Freund 's adjuvant were used when immunized the rabbit with amelogenin A 1 subcutaneously ;

结论：高浓度的Ⅱ型胶原加不完全佐剂给药组是较为理想类风湿关节炎大鼠模型，中医辩证属寒湿痹症，与之相比较，佐剂关节炎模型接近于湿热痹症。

Conclusion : The group injected with high concentration of type ⅱ collagen and incompleted Freund 's adjuvant is the ideal RA model . It is belonged to the symptom of cold-dampness arthralgia , compared with this model , the AA approaches the symptom of heat-dampness arthralgia .

结果：低浓度Ⅱ型胶原和完全佐剂组大鼠关节肿势迅猛，近似于佐剂关节炎大鼠模型，高浓度Ⅱ型胶原和不完全佐剂组大鼠关节肿势和缓；

Results : The rat joints swell of the group with low concentration of type ⅱ collagen and completed Freund 's adjuvant was rapid and severe , while that of the group with high concentration of type ⅱ collagen and incompleted Freund 's adjuvant was moderate and lasting .

用以上提取的鸡抗AIVIgG作为免疫原，制备完弗氏全佐剂和不完全弗氏佐剂疫苗免疫8周龄雌性SPFBALB/c小鼠。

With the chicken-derived anti-AIV IgG as the immune , the Freund 's complete adjuvant ( FCA ) vaccine and the Freund 's incomplete adjuvant ( FIA ) vaccine were prepared to incubated female SPF BALB / c mice of 8 weeks .

方法：应用不完全弗氏佐剂诱导小鼠腹腔淋巴管瘤形成，消化法分离获得LEC，置于自制的鼠尾胶包被的培养瓶（板）中培养。

METHODS : Mouse lymphangiomas in abdominal cavity were induced by incomplete Freund 's adjuvant , then disrupted and digested to obtain LEC , which were cultured in the flask or plate previously coated with rat-tail collagen .

方法将马疫锥虫kDNA与不完全弗氏佐剂乳化混合，通过皮下、腹腔、肌肉及静脉等途径注射入正常BALB/C小鼠。

Methods The emulsive mixture of TE kDNA and incomplete Freund ′ s adjuvant ( IFA ) was injected into normal BALB / c mice subcutaneously , intraperitoneally , intramuscularly or intravenously . These mice were immunized once every five days .

卵白蛋白组：非自身抗原卵白蛋白加不完全福氏佐剂，卵白蛋白用量200μg/只。

Ovalbumin group : Each rat was treated with autoantigen ovalbumin ( 200 μ g ) plus incomplete Freund adjuvant ;