凝胶过滤法

凝胶过滤法分离纯化大豆蛋白肽

Separation and purity of the soybean peptide by gel filtration chromatography

用凝胶过滤法研究了DPI的性质，说明接近生理环境的条件下（pH7.0，离子强度0.3），DPI没有自缔合性质，而是以单体存在。

The molecular weight of DPI was studied by the gel filtration method . The results demonstrate that DPI does not exhibit self-association behaviour , and exists as monomer under the experimental conditions .

用凝胶过滤法和SDS电泳法测得分子量为54000，PAS染色证实为糖蛋白。

The hyaluronidase was a glycoprotein ( positive PAS staining ) with a molecular weight of 54,000 which was determined by gel filtration on Sephadex G-75 and SDS-discontinuous polyacrylamide gel slab electrophoresis .

本实验成功地从小牛脾脏中提取了粒细胞生成抑制因子（GIF）的粗制品，并用交联葡聚糖G-10凝胶过滤法对其进行了初步纯化。

A factor specifically inhibition granulopoiesis was successfully extracted from calf spleen and partially purified by means of Dextran G-10 gel chromatograph .

经过对该菌株的发酵培养，将产生的多糖经Savage法、季铵复合物沉淀法、DEAE-纤维素（DE52）离子交换层析法及SephadexG-75凝胶过滤法分离纯化。

Polysaccharide produced by the mutated strain of Streptococcus zooepidemicus was purified by the procedure including Savage method , quaternary ammonium compound precipitation , DEAE-cellulose ( DE52 ) column chromatograph and Sephadex G-75 gel filtration .

采用盐提、热变性处理及凝胶过滤法从鹿花盘中提取鹿花盘蛋白质（PSAB）。

Protein component of sika deer antler base ( PSAB ) was extracted by means of salty extraction , boiling and gel filteration .

，以凝胶过滤法测定了其分子量均在4万左右。完全酸水解后气相色谱分析显示ABJ-1含有甘露糖、葡萄糖、半乳糖等单糖，ABJ-3含有大量的甘露糖及少量的葡萄糖等单糖。

Their molecular weight were around 40000 . The results of gas phase chromatographic analysis after complete acid hydrolysis showed that the composition of ABJ-1 was mannose , glucose , galactose and so on , and the composition of ABJ-3 was mannose and a few glucose .

用电泳及凝胶过滤法鉴定为均一组分，系由单一葡萄糖残基构成。

It was shown to be homogeneous by electrophoresis and gel filtration .

凝胶过滤法测定分子量；

Its molecular weight was measured by gel filtration .

凝胶过滤法纯化裂褶多糖检测方法的改进

Improvement of the Determination Method of Schizophyllan in Purification Process by Gel Filtration Chromatograph

采用凝胶过滤法和高效液相法得到荷叶多糖的分子量约为165000。

Its molecular weight was about 165,000 , which was determined by gel-filtration method and HPLC .

凝胶过滤法测定新型抗癌药硒化多烯脂肪酸多相脂质体的包封率

Determination of encapsulating rates of new antitumor agents & polyphase liposome of polyunsaturated fatty acids containing selenium by sephadex filtration method

分别采用葡聚糖凝胶过滤法、透析法、超速冷冻离心法分离脂质体和游离药物，计算脂质体包封率。

The liposomes and free drug were separated by Sephadex filtration , dialysis and refrigerated ultracentrifugation , respectively , then the entrapment efficiency of the liposomes was calculated .

菊糖样品经凝胶过滤法鉴定为均一组分，纯度较高，单糖组成仅含有果糖及少量葡萄糖，具有菊糖类物质的一般特征。

Inulin sample was identified as homogeneous component by gel filtration , and the purity was high . The monosaccharide composition only included the fructose and the little glucose , and it had the general characteristic of inulin class material . 7 .

采用凝胶过滤法和超强磁分离法对制备的纳米磁颗粒进行纯化，对2种纯化方法进行比较，最后选择具有操作简便、快速和价格低廉等优点的超强磁法进行纳米磁颗粒纯化。

The prepared nanoparticles were purified using gel filtration and high-intensity magnetic separation method , and the results of the two purification methods were compared . Finally we selected the high-intensity magnetic separation method to purify nanoparticles for its advantages , such as easy , fast , and cheap .

SDS-PAGE电泳和高效凝胶过滤色谱法研究人参蛋白热稳定性

Study on the Thermal Stability of Ginseng Protein by SDS-PAGE and Gel Filtration Chromatography

运用水油提、盐析及凝胶过滤色谱法对幽门螺杆菌YC－11株所分泌的形成空泡细胞毒素进行了提取。

The vacuolating Cytotoxin from Helicobacter pylori YC-11 was purified by using water-extracting , salt precipitation and gel filtration chromatographic fractionation .

利用红外光谱法、凝胶过滤色谱法和阳离子交换平衡法，分析了甘氨酸锰的红外光谱特征、甘氨酸锰和蛋氨酸锰在pH7、0.1Mtris缓冲溶液中的络合率。

The percentages of complexed Mn in Mn-Gly and manganese methionine chelate ( Mn-Met ) were determined in 0.1 M Tris buffer ( pH7 ) by gel filtration chromatography and cation exchanging equilibrium .

用吸附色谱法、凝胶过滤色谱法等方法对该活性成分进行分离和纯化，并用高效液相色谱法、薄层色谱法和化学方法对其纯度进行检验，得到2个纯物质HG1和HG2。

The bioactive substances were purified through adsorption chromatography , gel chromatography and so on . The purity tests of HG-1 and HG-2 by TLC , analytical HPLC and chemical methods proved that they were both single substance .

唑啉头孢菌素及其中间体的凝胶过滤分析法

Analysis of Cefazolin and Its Intermediates by Gel Filtration Chromatography

凝胶过滤色谱法研究酶解过程中木聚糖分子结构的变化

The Changes in the Structure of Xylan during Enzymatic Hydrolysis with Gel Filtration Chromatography

凝胶过滤色谱法测定泥鳅多糖的组成及分子量

Component Analysis and Molecular Weight Determination of Misgurnus Anguillicaudatus Polysaccharides by Gel Filtration Chromatography

采用高效液相凝胶过滤色谱法（HPGFC）测定这3个菌株提取物云芝胞内多糖的相对分子质量，并加以比较。

Their molecular weights were also determined and compared with high performance gel filter chromatography .

高效凝胶过滤色谱法测定玉米蛋白酶解产物寡肽的分子量分布

Assay on the Molecular Weight Distribution of Oligo peptide in Corn Protein Hydrolysate with Gel Filtration Chromatography

使用离子交换和凝胶过滤色谱法从富硒螺旋藻中提纯含硒藻蓝蛋白。

Selenium-containing phycocyanin ( Se-PC ) was purified from Se-enriched Spirulina platensis using ion exchange and gel filtration chromatography .

目的：建立高效液相凝胶过滤色谱法测定偶联物的偶联率、中间产物的生成量；

OBJECTIVE To establish a gel filtration chromatography ( GFC ) for determination of conjugation rate and yield of intermediate ;

采用凝胶过滤色谱法研究了木聚糖在酶解过程中分子量分布的变化。

The changes of the molecular weight distributions of xylan during enzymatic hydrolysis have been investigated with gel filtration chromatography .

结论：本实验建立的高效液相凝胶过滤色谱法简便、快速、灵敏、准确，对优化实验有指导意义，使偶联率得到了提高。

CONCLUSION The established method is simple , rapid , sensitive and accurate for optimizing experimental condition and increasing the conjugation rate .

本课题建立了一种新的蜂毒素提取纯化工艺，单纯采用凝胶过滤色谱法从蜜蜂毒中分离纯化出高纯度蜂毒素；

In this paper , we have established a new technique to extract and purify melittin from bee venom , in which only gel filtration chromatography was used .

本文采用离子交换色谱法及凝胶过滤色谱法对铬鞣废液中络合物组份、电荷、含量和分子大小及其在放置中的变化进行了研究。

The components , charges , molecular sizes and percent contents of complexes in used chrome liquor and their changes in ageing were studied by ion exchange and gel filtration chromatography .