分批培养

E.coliHB101（pBV-IL-6）的流加分批培养及其产物纯化

The Fed Batch Cultivation of E.coli HB101 ( pBV-IL-6 ) and the Purification of Product

目的：在FUS-50L生物反应器中利用补料分批培养技术培养表达含重组质粒pET28a（+）-GNLY的大肠杆菌BL21（DE3）pLysS株，生产重组颗粒溶素（GNLY）。

Objective : To prepare recombinant granulysin ( GNLY ) by fed batch cultivation of E. coli BL 21 ( DE3 ) pLysS in a FUS-50L bioreactor .

Logistic方程在微生物分批培养动力学中的应用

The Application of Logistic Equation in Kinetics of Microbial Batch Culture

分批培养时pH和温度对重组大肠杆菌生产谷胱甘肽合成酶系的影响

Effects of pH and temperature on the production of glutathione synthetases by recombinant E.coli in batch culture

酿酒酵母重组人α2a干扰素补料分批培养

Production of Recombinant Human Interferon α 2a by Saccharomyces cerevisiae in Fed-batch Culture

比较了酵母菌发酵生产谷胱甘肽（GSH）的几种补料分批培养方式。

Several feeding fed-batch culture methods for the production of glutathione ( GSH ) by Saccharomyces cerevisiae were compared .

Candidakrusei的分批培养与补料分批培养生产甘油

Production of glycerol in batch and fed-batch cultivation processes of Candida krusei

研究了分批培养条件下固定CO2氢细菌的生长及底物消耗动力学特性。

The kinetics of the microbial growth and the substrate consumption is studied in the batch culture system using CO 2 as a sole source of carbon .

然后确定了反复补料分批培养时更换新鲜培养基的最佳时间为每次培养进行48h后、体积比例为90%。

In repeated fed-batch culture , the optimized time and volume for refreshing culture was 90 % per 48 hours .

在分批培养中，最适初始pH值为9，最适温度30℃，钙离子和镁离子对其酶活性有促进作用。

In the batch culture , the optimal temperature was 30 ℃ and optimal pH was 9 . Ca ~ ( 2 + ) and Mg ~ ( 2 + ) were found to stimulate the keratinase activity .

在分批培养和补料分批培养期间，采用不同的pH控制模式：在发酵前期采用分批培养，加入碱以补偿pH值的降低；

PH was controlled by different pH controlling mode during batch culture and pH stat fed batch culture . The addition of alkali could keep pH constant to compensate the decrease of pH during the first phase of culture .

实验结果表明：同时设置发酵液的pH上限和下限可避免恒pH补料分批培养过程中葡萄糖的周期性缺乏问题；

The research results shown that : the pH controlling strategy , which pH high limit and pH low limit was set at one time in the experiment , could avoid periodical absence of glucose during fed batch culture ;

在液体分批培养体系中，研究了药用蕈菌姬松茸Agaricusblazei悬浮菌丝物的形态多样性及其生长特征。

The morphology and growth of the suspending mycelia of medicinal mushroom Agaricus blazei in liquid submerged batch culture were studied .

研究了2.5L罐分批培养时pH和温度对重组大肠杆菌生产谷胱甘肽合成酶系的影响，确定了分批培养时生产谷胱甘肽合成酶系的最佳pH和最佳温度。谷胱甘肽合成酶在大肠杆菌中的高效表达及性质

Effects of pH and temperature on the production of glutathione synthetases by recombinant E. coli were investigated , and the optimal pH and optimal temperature was obtained . Study on the Glutathione Synthetases Expression in Recombinant Escherichia coli and Properties

用基于△规则和最速下降法的反向传播算法构建了一个能够超前1h预测青霉素补料分批培养状态变化的人工神经网络模型。

Neural network model was constructed by using a back propagation algorithm on the delta rule and gradient descent , which could predict the state variables of penicillin fermentation in fed batch one hour ahead .

采用溶氧反馈的分批培养流加补料的方法高密度培养重组大肠杆菌BL21（DE3）生产重组葡激酶-水蛭素融合蛋白。

Recombinant fusion protein staphylokinase-hirudin has been produced by adopting the dissolved oxygen feed-back fed-batch with the high cell density cultivation strategy in E. coli BL21 ( DE3 ) .

方法采用补料分批培养方法，在5L发酵罐中对发酵工程菌GS115/Fab进行高密度发酵，发酵温度28～30℃，pH值范围为50～55，溶氧范围20%以上；

Method The high density fermentation of genetically engineered Pichia pastoris was proceeded in a 5L bioreactor using fed batch fermentation . The fermentation temperature was set at 28-30 ℃, the pH was 5 0-5 5 , and the DO was kept over 20 % .

在5L自动发酵罐中进行补料分批培养，最终菌体浓度达到45×109cfu/mL，芽孢浓度为12×109cfu/mL，分别是分批培养的59倍和38倍。

High-cell-density fed-batch culture was operated in 5L auto-fermentor , the final cell concentration was 4.5 × 10 9cfu / mL , spore concentration was 1.2 × 10 9cfu / mL , which was as much as 5.9 times and 3.8 times of batch culture respectively .

苏云金杆菌深层发酵补料分批培养工艺研究

Study on the Feed Batch culture technology of Bt submerged fermentation

青霉素补料分批培养的人工神经网络模型

Artificial Neural Network Model for Penicillin Fermentation in Fed batch

补料分批培养海洋红酵母的研究

Study on Fed - batch Culture of Oceanic Red Yeast

白介素2工程菌的分批培养及高效表达

High expression of recombinant interleukin 2 in E. coli in batch culture

采用补料分批培养技术可降低果糖和葡萄糖的抑制。

The fed - batch culture can eliminate the inhibition .

利用补料分批培养技术生产红曲色素的研究

Study on Feed batch Culture to Produce Monascus Pigment

同时研究了细菌纤维素生产过程中分批培养阶段的操作条件，然后确定了细菌纤维素流加发酵的操作参数。

Meanwhile the operational conditions of batch culture stage in BC fermentation were studied .

补料分批培养过程模拟

The Simulation of Fed - batch Process

微生物的培养方式分为分批培养、半连续培养和连续培养，其中，分批培养应用最为广泛。

Microbial cultivation methods can be classified into batch culture , semi-continuous culture and continuous culture .

由分批培养确定了适于常规发酵的培养基及培养条件。

The glucose-urea medium and dark-aerobic condition were determined by batch culture to fit normal fermentation .

采用一种新型溢流喷射光生物反应器进行螺旋藻的室内分批培养试验。

A new type of overflowing jetting photobioreactor was used for the indoor batch culture of Spirulina .

氮饥饿补糖分批培养小克银汉霉产γ-亚麻酸的研究

Study on Fed-batch Culture of Strain Cunninghamella sp. to Produce Gamma-linolenic Acid by Nitrogen Starvation and Glucose Feeding