离子交换层析

超滤透析后，再通过串联离子交换层析和固定化金属螯合亲和层析，对蛋白C进行纯化。

After ultrafiltration and dialysis , protein C was purified further with ion exchange chromatography and immobilized metal affinity chromatography .

采用SephadexG50凝胶层析、CMSepharoseCL6B离子交换层析及反相高压液相色谱等步骤从鲢鱼鱼精蛋白的粗品中分离得到一种具有抗菌活性的蛋白质。

Chub protamine was purified by Sephadex G_50 gel filtration Chromatography , CM_Sepharose CL_6B ion exchange Chromatography and RP_HPLC .

方法在已有的凝胶过滤和离子交换层析的基础上，进一步通过亲和层析和凝胶过滤分离纯化人的SC并进行相应的鉴定。

Methods Ionic exchange chromatography , gel filtration , and affinity chromatography were used for purification of human SC.

离子交换层析纯化出α-半乳糖苷酶，酶比活性从15U／mg提高到28.14U／mg。

The recombinant enzyme was purified by cation exchange chromatography and the activity was up to 28.14U/mg .

方法：在大肠杆菌中表达AnnexinB1蛋白，经离子交换层析得纯品；

Methods : Annexin B1 was expressed in E.

结论：亲和层析和离子交换层析都是纯化重组L1蛋白的适宜方法，二者纯化效率相当。

Conclusion : Affinity chromatography and ion exchange chromatography are both efficient methods to purify recombinant L1 protein .

在有氧条件下，利用QsepharoseFastFlow离子交换层析和BlueSepharoseCL6B亲和层析提纯克雷伯杆菌胞内甘油脱氢酶。

Glycerol dehydrogenase from Klebsiella pneumoniae was purified to homogeneity by Q Sepharose Fast Flow ion-exchange chromatography and Blue Sepharose CL-6B affinity chromatography under aerobic conditions .

离子交换层析初步分离大鼠肝蛋白水解酶、ADAMs、ACP、AKP和PCNA

Primary purification of proteinases , adams , acp , AKP and PCNA in rat liver by ion exchange chromatography

用CMSepharoseFastFlow离子交换层析结合SephadexG-75凝胶柱分离纯化RIP。

Through ion exchange chromatography of CM Sepharose Fast Flow and the gel chromatography of Sephadex G-75 , the RIP of balsam pear was obtained .

通过CMSephadexC50离子交换层析，对RX17溶菌酶粗制品进行了初步分离，得到了3个有溶菌活力的组分。

Through CM-Sephadex C-50 ion-exchange chromatography , three proteins with lytic activity were separated from the crude extract of RX-17 .

方法依次使用差速离心、热失活、离子交换层析的方法纯化体外重组的人tau蛋白；

Methods Human recombinant tau protein in vitro was purified by using differential centrifugation , heat inactivation and ion exchange chromatography .

本文报告用CM和DEAE纤维素离子交换层析从猪胰脏分离出对胰蛋白酶有特异性抑制作用的抑制剂。

In this paper the isolation and identification of trypsin inhibitors from porcine pancreas by CM-and DEAE-Cellulose ion exchange chromatography have been reported .

用棉铃虫组织蛋白酶B为酶源，通过硫酸铵沉淀、排阻层析和离子交换层析等方法，从大豆中分离纯化到了一种对HCB有抑制活性的蛋白酶抑制因子，命名为HCBSoyI。

An inhibitor , HCB_SoyI , purified from the soybean seeds by ammonium sulfate precipitation , gel filtration and ion_exchange chromatography , was here reported .

用1mlSP预装柱及自装28mlStreamlineSP柱进行离子交换层析以验证吸附效果。

The adsorption effect was then verified by cation-exchange chromatography using 1-ml Streamline SP prepacked column and 28-ml Streamline SP self-assembly column .

进一步通过离子交换层析DEAESepharoseCL-6BFastFlow后，得到高纯度、高比活力的精氨酸激酶，最终的纯化倍数为11.9倍，酶活力回收率为80%。

Further purification was achieved by DEAE Sepharose CL-6B Fast Flow anion exchange chromatography ; ultimately AK was purified up to s 11.9 with a recovery of 80 % .

用DEAE-52离子交换层析分离到载脂蛋白的A-I（apoA-I）的多态性。

Polymorphs of apolipoprotein A-I ( apoA-I ) were isolated by DEAE-52 ion-exchange chromatography .

为了提高脂肪酶的酶活水平，实验采用简单的两步法，即离子交换层析和疏水层析法对candidasp.99-125脂肪酶进行了纯化。

The extracellular lipase from Candida sp. 99-125 was purified by ion exchange chromatography and hydrophobic interaction chromatography .

采用硫酸铵盐析、DEAE离子交换层析纯化牛初乳免疫球蛋白IgG，并对其进行胰蛋白酶体外消化研究。

Immunoglobulin G is purified by salting out , gel chromatography , and DEAE ion exchange chromatography from bovine colostrum and hydrolyzed by trypsin in vitro .

进一步经过硫酸铵分级沉淀，DEAE-sepharoseFastFlow离子交换层析，Sephacryls-200凝胶过滤，获得电泳纯的杀虫蛋白。

Insecticidal protein from Serratia marcescens HR-3 was purified by ammonium sulfate precipitation , DEAE-Sepharose Fast Flow , Sephacryl S-200 gel filtration and native SDS-PAGE cuting method .

采用聚乙二醇沉淀、柠檬酸钡吸附、DEAE纤维素离子交换层析等方法，制备了猪凝血因子V。

The technology including polyethylene glycol precipitation , barium citrate adsorption and DEAE-cellulose chromatography were applied to prepare porcine factor V . Porcine factor V was identified by SDS-PAGE .

采用离子交换层析、凝胶层析，以SDS-PAGE检测，对其中一个蛋白进行了分离纯化研究，得到了32kDa蛋白的纯化工艺和参数。

The purification technology and parameters for 32 kDa were researched with ion exchange chromatography and gel chromatography , detected with SDS-PAGE .

应用全自动糖化血红蛋白批量检测仪构建的低压离子交换层析梯度洗脱分析系统测定其糖基化血红蛋白A1c（HbA1c）水平。

The glycosylated hemoglobin ( HbA1c ) was determined by low pressure cation exchange chromatography in conjunction with gradient elution system .

然后，经过包涵体溶解、复性、浓缩及DEAE离子交换层析，使γ干扰素纯化。

By the inclusion bodies dissolution , renaturation , concentration and DEAE ion exchange chromatography IFN - γ was purified .

依次经PSL－Sepharose亲和层析柱和纤维素CM－52离子交换层析柱，从猪精子的CHAPS抽提液分离得4个蛋白质组分。

Four protein components have been isolated from the CHAPS extract of boar spermatozoa by chromatography on PSL-Sepharose affinity column and cellulose CM-52 ion exchange column sequentially .

单峰胰岛素经DEAE琼脂糖快流速离子交换层析后用醋酸锌结晶，得到结晶单组分胰岛素。

Single peak insulin was purified by using DEAE - Sepharose fast flow ion exchange chromatography and crystallizing with zinc component .

离子交换层析纯化的重组Fab，纯度为97%，回收率为75%～85%。

The purity of recombinant Fab fragment purified by ion exchange chromatography is 97 % , and the recovery rate of the Fab antibody is about 75 % ~ 85 % .

运用SephadexG-75层析和离子交换层析两步法分离纯化眼镜蛇蛇毒，分离效果好，纯度高。

The better effect of separation of naja naja Atra venoms and high qualities was acquired by Sephadex G-75 chromatography and ion-exchange chromatography . 3 .

结论试管法选择的离子交换层析的最适吸附条件用于从大肠杆菌中初步分离纯化anti-HBsAgFab切实可行。

Conclusion The optimal condition of cation-exchange chromatography selected by test tube method can be applied for separation and purification of anti-HBsAg Fab fragment from E. coli .

辛酸-硫酸铵盐析及DEAE离子交换层析分离、纯化兔抗血清。

And the SARS-CoV specific anti-serum was purified according to the method of octanoic acid-ammonium sulfate salting out and DEAE ion exchange chromatography .

方法采用分级盐析、离子交换层析及凝胶过滤方法，分离纯化了正常大鼠脾脏20S蛋白酶体，并进行透射电镜观察。

Methods 20S proteasome was isolated and purified with salt fraction , anion exchange column chromatography and gel filtration methods and its morphologic features were observed with transmission electron microscopy .