基础培养基

C组：仅应用内皮基础培养基培养。

C group : endothelial basal medium .

B组：在内皮基础培养基中添加生长因子VEGF、bFGF；

B group : endothelial basal medium with VEGF and bFGF ;

对培养保加利亚乳杆菌的基础培养基进行筛选，确定为MRS培养基。

MRS medium was found during several radical mediums to cultural Lactobacillus bulgaricus .

基础培养基组成：含青霉素、链霉素、两性霉素B及体积分数为0.02B27添加液的DMEM/F12。

Basal medium was composed of penicillinum , phytomycin , amphotericin B and DMEM / F12 containing B27 annex solution of 0.02 volume fraction .

MS和B5均可作为基础培养基使用。

MS and B5 medium could both be used as the base medium .

本文选用MRS培养基作为基础培养基，在此基础上利用单因素实验确定了最佳碳源为葡萄糖，最佳氮源为蛋白胨。

This study chosen malt extract MRS as base medium . The optimal carbon source was determined by single factor was glucose , and the optimal nitrogen source was peptone .

与MEM相比，M199更适合于用作培养猪血管内皮细胞的基础培养基；

M 199 was more appropriate as culture medium to SVEC than MEM .

综上结果说明，A3培养基是一种适宜大肠菌群生长，抑制革兰氏阳性菌生长的初发酵基础培养基，能在短期内使发酵管产气的菌群基本就是大肠菌群。

A3 was a kind of initial fermentation media which was good for Coliform bacteria detection and bad for the growth of grams positive bacteria .

在培养的2～5h内，补充核苷酸均能提高基础培养基（P<0.05）和完全培养基（P<0.01）中DNA修复细胞的百分率。

The percentage of DNA-repaired cells was increased significantly with nucleotides supplementation between 2 and 5 h in basic ( P < 0.05 ) or complete medium ( P < 0.01 ) .

方法预先制备同种异体脱钙骨，取健康成人骨髓，用单核细胞分离液分离MSC，间充质干细胞基础培养基原代和传代培养。

Methods Allogenic decalcified bone matrix was prepared . MSCs were isolated from adult human bone marrow with monocyte-separating medium and then cultured in basic medium of mesenchymal stem cells ( MSC ) .

实验证明：该菌株在基础培养基上培养72h以后，可以产生大量的伴孢晶体，晶体的大小为0.5～1.0μm，形状为菱形。

The results proved that : it can produce a lot of parasporal crystal after 72h in BP medium . The size of crystal is 0.5 ~ 1.0 u m. Its shape is rhombus .

丙烯酰胺处理组在基础培养基中添加0、50、100、500、1000μM的丙烯酰胺，处理1小时后，检测细胞中活性氧（ROS）与总谷胱甘肽过氧化物酶含量。

The acrylamide-treated groups were added 0,50,100,500 and 1000 μ M concentrations of acrylamide in the basic medium respectively . After 1 hour treatment , the content of reactive oxygen species ( ROS ) and total glutathione peroxidase in cells were examined .

将小鼠5d龄ES-D3细胞源的类胚体（EBs）单细胞，按5×104/mL接种6孔细胞培养板，在DMEM基础培养基中使EBs单细胞贴壁培养。于第14！

The cells of 5-day embryoid bodies ( EBs ) derived from murine ES-D3 lineage were inoculated into each well of 6-well culture plate at 5 × 104 / mL in DMEM .

基础培养基筛选显示HSVA为最优培养基。

The HSVA was identified to be the optimized culture medium by the experiment of filtrating basic culture medium .

D/F12-EB基础培养基较其它培养基更能支持生殖细胞的体外分化，Wnt3a和BMP4能有效促进原始生殖细胞的形成。

Compared with other medium , D / F12-EB base medium is more likely to support the differentiation of germ cell in vitro .

与FAD培养基和基础培养基比较，K-SFM培养的细胞呈单层生长，数代后细胞形态与原代细胞仍然十分相似，且生长较快。

Compared with FAD and basic medium , cells cultured by K-SFM growed with single layer and growed fast . After several generations , morphous of cells was similar with that of initial generation .

方法：（1）0.25%分离酶，0.125%胰酶0.01%EDTA两步酶法分离包皮角质形成细胞，按不同密度接种，K-SFM、FAD培养基和基础培养基分别培养，观察原代融合生长时间，传代生长情况。

Methods : ( 1 ) The samples of circumcision were separated by 0.25 % dispase , 0.125 % Trypsin and 0.01 % EDTA sequentially . According to different inoculation density , keratinocytes were cultured by K-SFM 、 FAD and basic medium .

方法：通过摇瓶试验筛选适合重组人干细胞因子工程菌DH5α（pMG604）生长和产物表达的基础培养基，在此基础上进行了分批补料批培养和连续补料批培养的研究。

Methods : For production of rhSCF , a medium for the recombinant Escherichia coli DH5 α( pMG604 ) growth and rhSCF production was screened by culture test in flask , and the batch-feed fermentation and continuance-feed fermentation technology were studied .

产酶条件研究表明：在pH7.0的基础培养基中添加2.0%的细粉几丁质，1.0%的酵母膏，220rpm30℃下培养72小时，几丁质酶的产出最大。

The study on the synthetic conditions for the chitinase showed that the most chitinase was synthesized when WB 50 was cultured for 72 hours ( 220rpm ) in a basic medium ( pH 7.0 ) added by 2.0 % chitin and 1.0 % yeast extract .

一种新的真菌用基础培养基&维乐菜汁培养基

A New Culture Medium for Fungi & Weile Juice Agar

基础培养基影响细胞的最大活细胞密度，不影响生长速率。

The basal media affected maximum viable cell density , not specific growth rate .

产气荚膜梭菌和腐败梭菌在厌氧菌基础培养基中的生长特性

Characters Growth of Cl. perfringens and Cl. Septicum Cultivated in the Basic Medium of Anaerobic Bacteria

发酵液效价达到1456.8μg/mL，比基础培养基效价提高近10倍。

The potency reached 1456.8 μ g / mL and was about 10 times higher than that of basic medium .

紫球藻在海水基础培养基中生长缓慢，培养密度低，这就为从其细胞内提取活性物质增加了成本。

Cruentum cells grow very slowly in the natural and artificial seawater medium , and the biomass density is also lower .

优化培养基液体发酵液中灵芝漆酶活性是液体发酵基础培养基的25.72倍；

After culture medium optimization , Ganoderma lucidum laccase activity of submerged fermentation liquid is 25.72 times as basic liquid medium ;

研究了城市污水处理系统中微生态种群结构在无机培养基和基础培养基条件下受到无机硝酸盐冲击的情况，并分析了微生物群落结构及行为特征。

The community structure and the behavior characteristic of the activated sludge in mineral substrate and in basic substrate conditions are studied .

硅藻发酵生产二十碳五烯酸基础培养基的选择研究二十碳五烯酸对高糖与去甲肾上腺素诱导的心肌细胞肥大的抑制作用

Studies on Selecting of Different Mediums for EPA Production by Diatom Nitzschia laevis Eicosapentaenoic acid prevents cardiomyocyte hypertrophy induced by high glucose and NE in vitro

采用光密度测定的菌株生长值和农药降解率的关系曲线表明，在基础培养基中，农药降解和菌株生长成正相关，说明菌株能以拟除虫菊酯类农药为唯一碳源和能源进行生长。

Growth measured as optical density showed a positive correlation to the amount of pesticide removed , suggesting the bacteria used these pyrethroids as carbon and energy sources .

原代培养1周后，获取体外稳定增殖的鼠脊髓源性神经干细胞克隆，在基础培养基中加入体积分数为0.1的胎牛血清作为对照。

1 week after primary culture , in vitro spinal cord derived NSCs clone was obtained . Feta calf serum of 0.1 volume fraction was added in the basal medium .

培养液上清液对玉米螟初孵幼虫的体重抑制率为9081%，比基础培养基的杀虫活性提高了46.5%。

Rate of growth inhibition of neonate larvae of Ostrinia furnacalis was 90.81 % . It was increased by 46.5 % in comparison with basic culture medium ( TSB ) .