小孢子

小白菜小孢子培养再生植株的倍性与基因组DNA甲基化的关系

Studies on Relation between DNA Methylation and Ploidy of Plant Regeneration from Microspore of Pakchoi

玉米基因雄性不育系（ms2/ms2）小孢子败育的细胞学研究

Cytological Studies on Microspore Abortion in Genie Male-Sterile Line ( ms_2 / ms_2 ) of Corn

2个甘蓝F1小孢子培养中高出胚率的诱导技术研究

Studies on High Embryo Rates Induction Technique of Microspore Culture in Two Cabbage F_1

（普通小麦/长穗偃麦草）F1小孢子发生和雄配子体发育的细胞学特点

Cytological characteristic on Microsporogenesis and male gametophyte development of ( t.aestivum/e.elongata ) f 1

试验共获得8对含有多态性的引物，试验结果证明小白菜小孢子培养获得的再生植株的倍性与基因组DNA甲基化有关。

It indicated that the ploidy level of regeneration plants from pakchoi may be related to DNA methylation in plant genomics .

卫星搭载凤仙花SP1代单株小孢子变化的比较研究

Comparison of microspores in SP_1 generation mutant plants from space-flight induced Impatiens balsamina seeds

利用小孢子培养和诱变技术改良甘蓝型油菜CMS恢复系

Utilizing Microspore Culture and Mutagensis to Improve CMS Restore Line in Brassica Napus

培养24h后，小孢子体积增大。

After 24 h in culture , the microspores had increased in size .

低温处理后，3个品种小孢子的异常率以及花粉和花药的败育率都以E品种最高，X品种次之，N品种最低。

The abnormal rate of formation and development of microspore pollen and anther abortion are highest in E , next in X , lowest in N.

叶用芥菜小孢子培养热激处理的适宜温度为33℃，时间为24h。

The optimum temperature of leaf mustard heat shock treatment was 33 ℃, 24h .

利用光镜和透射电镜对同一核背景的ms2不育花药和正常可育花药进行了小孢子发育的细胞学比较观察。

Comparative observations on microspore development in ms2 genie male-sterile line and its fertile counterpart were made with light and transmission electron microscopy .

2,4-D对小孢子胚的形成有强烈的抑制作用。

2,4-D strongly inhibited the formation of microspore embryo .

四分体到小孢子单核期和小孢子二核后期至成熟花粉期不育花蕾ABA含量仍高于可育花蕾。

From tetrad stage to microspore stage , the ABA content in sterile buds was higher than fertile buds .

用孚尔根核染色反应处理DNA的变化：小孢子母细胞的核是鲜紫红色，细胞质无色；

The treatment with Feulgen reaction shows that the DNA of undergo the following change : The nuclei of microspore mother cells are of fresh purply red color and the protoplasms are colorless ;

PolTCMS小孢子培养体系的建立与温敏基因的QTL定位

Establishment of Microspore Culture System for Pol TCMS and QTL Mapping of Its Temperature-Sensitive Genes

羊草（Leymuschinensis（Trin）Kitag.）的小孢子发生和雄配子体形成

The Microsporogenesis and the Formation of Male Gametophytes in Leymus Chinensis ( Trin . ) Kitag

杜仲（EucommiaulmoidesOliv）小孢子母细胞减数分裂属同时型。

The meiotic division of the microspore mother cell of Eucommia ulmoides Oliv belong to simultaneous type .

对玉米Mo（17）核背景三种雄性不育细胞质（T、C、S）系及正常可育细胞质（N）系小孢子发育过程中花药组织的物质代谢系统进行了研究。

The material metabolic system of isonuclear alloplasmic lines on M_ ( o17 ) nuclear background with three male & sterile cytoplasms T. C. Tang Xu ( S ) and normal ( N ) cytoplasm was studied .

在这个时期小孢子的细胞核出现了异常的降解现象，而且在整个小孢子发育中都无法检测到明显的细胞核，即产生了异常的PCD现象。

During this period , the nucleus of microspore abnormally degraded , and afterward no nucleus were observed , suggesting occurrence of PCD .

中央大液泡的形成使得小孢子具有极性，极性小孢子有丝分裂一次形成二胞花粉，在细胞核（营养核及生殖核）中分布的ATPase较多，细胞质中的ATPase较少。

The mitosis of polar microspore formed a 2-cellular pollen grain . ATPase precipitates were more in both nuclei of vegetative cell and generative cell but less in the cytoplasm .

结论如下：在花药发育过程中，小孢子母细胞和四分体内的ATPase很少，仅在细胞核及小液泡中分布有少量的ATPase。

The results are as follows : During anther early development , only few ATPase precipitates located in small vacuoles and the nucleus of microspore mother cell and tetrad microspore .

若象大白菜、甘兰型油菜那样通过游离小孢子培养的方法获得双单倍体（DoubleHaploid；DH）植株，可大大缩短纯化菜心品种所需的时间并减少工作量。

With the method of anther and pollen culture , it can be got double haploid plant . Therefore , it may greatly shorten the time of purification in the breeding .

三种松科植物（白皮松Pinusbungeana，油松pinustabulaeformis，白杄Piceameyeri）小孢子母细胞减数分裂中的次级配对和多极分裂现象

The Secondary Pairing and Multipolar Division in PMC Meiosis of Three Species ( Pinus bungeana , Pinus tabulaeformis , Picea meyeri ) of Pinaceae

小孢子单核期为适宜的诱导时期，培养基以6%～13%蔗糖浓度的B5培养基较好；

The uninucleate microspore stages and B5 medium with sucrose from 6 % to 13 % were optimal for callus induction .

品种的基因型不同，适宜的双核期小孢子比例也不同，‘bc－0’、‘Galaxy’在双核期小孢子比例分别为15.38%和13.60%时，出胚率最高；

Different genotypes had different proper binucleate pollen ratio . For ' bc-0 ' and ' Galaxy ' , 15.38 % and 13.60 % binucleate pollen ratio gave the highest embryo yield ;

具有21条染色体的小孢子（2x+1）再生植株能力与正常小孢子（2x）再生植株能力相近。

The plant regeneration rate of microspore which had 21 chromosomes was similar to the normal microspore which had 20 chromosomes .

通过检查小孢子母细胞减数分裂染色体行为和四分孢子行为，分析了热激或低温诱导产生2n花粉的细胞遗传学机理。

The cytogenetic mechanism of 2n pollen formation after heat-shock or low temperature treatment was determined on the basis of the behavior of meiotic chromosomes and sporads during microsporogenesis .

甜菜无融合生殖单体附加系M14的小孢子发生与雄配子体发育的观察

The observation of Microsporogenesis and the development of male gametophytes in the monosomic addition lines of apomictic beet M14

从花药培养、小孢子培养和子房培养几方面综述了构建烟草DH群体的基本途径及其在遗传育种中的应用和发展动态。

This paper briefly reviewed the main methods of establishment doubled haploid ( DH ) population by anther culture , microspore culture and so on and its application and processing in tobacco breeding .

玉米CMS-S小孢子败育过程中的细胞程序性死亡

Programmed Cell Death during Abortion of Microspore in S-type Cytoplasmically Male-sterile Maize