微球菌核酸酶
- 网络micrococcal nuclease;MCN;MNase
微球菌核酸酶
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方法:选用微球菌核酸酶(MCN)为染色质结构识别探针,经蔗糖密度梯度离心、凝胶电泳,可显示不同龄大鼠大脑染色质结构特征的变化。
Methods : Micrococcal nuclease ( MCN ) was used as the probe to study chromatin structure and agarose gel electrophoresis and sucrose density gradient centrifugation were employed to investigate the changes of cerebral chromatin structure in rats of different ages .
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应用有机汞亲和层析柱分离经微球菌核酸酶有限制消化的HL-60细胞核,得到有机汞层析柱亲和及不亲和的2个核小体组分。
The unbound and bound fractions of nucleosome from HL-60 cell nuclei digested with micrococcus nuclease were separated by Hg-affinity chromatographic column .