木瓜蛋白酶

甘蔗渣纤维素固定化木瓜蛋白酶及其应用研究

Study of bagasse - cellulose fixed - papain and its application

壳聚糖载体柔性固定化木瓜蛋白酶

Flexible Immobilized Papain on the Chitosan Support

金属配合物对DNA的切断作用木瓜蛋白酶水解饲料蛋白的最佳作用条件

The Study of Non-enzymatic Cleavage of DNA by Metal Complex The best hydrolytic conditions of forage proteins digested by papain

静态吸附法主要考察了缓冲液pH值、缓冲液钠离子强度、吸附温度和吸附时间等对木瓜蛋白酶吸附量的影响。

The static adsorption mainly concerned on the buffer pH , Na ionic strength , the adsorption temperature and adsorption time .

在甲醇溶液中木瓜蛋白酶催化酪蛋白水解反应的最适pH为8.5，最适温度为70℃；

The optimum pH of methanol solution was 8.5 and the optimum temperature was 70 ℃ for catalytic activity of papain .

木瓜蛋白酶的芳香族氨基酸残基在DNA大沟槽中与它的碱基之间的芳基堆积作用是引起吸收光谱变化和荧光猝灭的主要原因。

Papain interacted with the major groove of ctDNA . Aromatic stacking interaction is the main reason of change of absorption spectrum and fluorescence quenching of papain .

本文报道在两相溶剂体系中利用木瓜蛋白酶催化合成aspartame前体z－apm的研究。

Papain-catalyzed synthesis of aspartame precursor z-apm in biphasic solvent system wasinvestigated .

木瓜蛋白酶水解军曹鱼头蛋白制备ACE抑制肽的工艺优化

Optimization of technology for preparation of angiotensin-I-converting enzyme inhibitory peptides by hydrolyzing the Head of Cobia with Papain

除正常对照组外，其余3组右膝木瓜蛋白酶关节腔注射法建立OA模型。

Osteoarthritic models of rats were established by intraarticular injections of papain into the right knee joints of groups B , C and D.

采用木瓜蛋白酶处理的兔红细胞花环形成试验和兔抗豚鼠免疫球蛋白标记的免疫微球花环形成试验及乳胶颗粒吞噬试验分别测定了2～4用龄豚鼠胸腺的T淋巴细胞、B淋巴细胞和吞噬细胞。

Thymic T and B lymphocytes and phagocytes in 2 ~ 4 week-old newborn guinea pigs were determined by rosetting with papain-treated rabbit erythrocytes , immunobeads coated with rabbit anti-guinea pig immunoglobulin , and latex phagocytosis .

本文根据吸收、荧光和RRS光谱的变化并结合圆二色光谱和热力学数据讨论了木瓜蛋白酶与DNA之间相互作用的主要结合力、两者的结合位点和结合模式。

The interaction types and binding modes were discussed by characteristics of RRS , absorption , fluorescence and circular dichroism spectra combining thermodynamic data .

本文就木瓜蛋白酶作用于大豆分离蛋白、7S和11S蛋白溶液形成凝胶过程的流变性质进行了研究。

The rheological properties of 7S and 11S gels induced by papain were studied .

主要研究木瓜蛋白酶在pH值、水解时间、液固比、添加量不同因素条件下对碎米蛋白提取率和溶解度的影响。

The influence of different conditions on protein yield and protein solubility were researched at different factors such as pH , hydrolyzing time , ratio of liquid to solid , content of papain proteinase .

氯化钙、磷酸盐、木瓜蛋白酶对牛肉MFI及剪切力的影响

Effect of CaCl_2 , phosphate , papain on MFI and shearing force of beef

结果木瓜蛋白酶检出了抗D、抗E、抗C、抗c、抗e、抗Jka、抗Jkb7种抗体（7/12）；

Results Seven kinds of IgG red blood cell antibodies including anti-D , anti-E , anti-C , anti-c , anti-e , anti-Jk a , and anti-Jk b were detected using papain technique ( 7 / 12 ) .

介孔分子筛MCM-48固定化木瓜蛋白酶性质的研究

Immobilization of Papain on Mesoporous Molecular Sieve MCM-48

在最佳固定化条件下制得的固定化酶与游离酶相比，固定化酶的pH稳定性、操作稳定性和储藏稳定性都有了较大改善，有利于木瓜蛋白酶的重复使用。

Under the optimum immobilization conditions , the pH , operational and storage stabilities of immobilized enzyme are greatly improved compared to the free enzyme , which was beneficial to the reuse and storage of immobilized enzyme .

采用十二烷基酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）方法，分析了几种商品蛋白酶（包括枯草杆菌蛋白酶、胰蛋白酶、胰凝乳蛋白酶、木瓜蛋白酶和细菌碱性蛋白酶）对大豆分离蛋白（SPI）的降解模式。

SPI hydrolysates induced by several commercial proteinases ( subtilisin protease , chymotrypsin , trypsin , papain , and Protex ) were analyzed by the SDS-PAGE .

目的：探讨三种巯基蛋白酶（菠萝蛋白酶、木瓜蛋白酶和无花果蛋白酶）在不同盐浓度、pH值和温度环境中水解人全唾液生物膜的效率。

Objective : The aim of this paper is to investigate the efficiency of hydrolyzing human whole salivary biofilm by three thiol proteases ( bromelain , papain and ficin ) in different salt concentration , pH and temperatures .

当CPI与木瓜蛋白酶等摩尔结合后，CPI完全丧失抑制活性，复合物CD谱发生显著变化，α螺旋含量降低。

When acted with equal proportion of Papain , the inhibiting activity of CPI lost completely ; CD spectrum changed remarkably and the content of α - helix declined .

匀浆状甘蔗渣纤维素用NaOH活化后，顺次经过高碘酸钠、尿素、甲醛处理，成为高反应性固定化酶载体，可用于木瓜蛋白酶的固定化。

Homogenized bagasse-cellulose activated by NaOH and treated in turn with sodium periodate , urea , formaldehyde , was used in the fixation of papain as a fast reactional carrier .

报道了多孔硅球固定化猪胰脂肪酶（IE）催化一步合成侧链含活性羧基的聚（酯碳酸酯）的方法.用载体交联法制备了多孔硅球固定化木瓜蛋白酶。

One-step synthesis of poly ( ester-carbonates ) containing pendant carboxyl groups had been successfully performed using immobilized lipase on porous silica beads as catalysts . Papain has been immobilized on porous silica beads by crosslinking method .

方法：采用胶原酶Ⅰ（1mgmL）、木瓜蛋白酶（5mgmL）消化分离大鼠肺内动脉平滑肌细胞；

Methods : Single isolated smooth muscle cells from rat intrapulmonary artery were obtained by employing collagenase ⅰ( 1 ? mg / ml ) and papain ( 1 ? mg / ml ) .

木瓜蛋白酶（Papain）是一类巯基蛋白酶，广泛地存在于番木瓜的根、茎、叶和果实内，未成熟果实的乳汁中含量最丰富。

Papain is a kind of SH-protease , it widely exists in papaya roots , stems , leaves and fruits , the milk of immature fruit is the most abundant .

目的比较木瓜蛋白酶、抗球蛋白实验、聚凝胺和微柱凝胶4种检测IgG红细胞抗体方法的特异性、敏感性、效价及所需时间。

Objective To compare the specificity , sensitivity , titers , and rapidity of four methods including papain technique , anti-globulin technique , polybrene test , and micro-column gel test for determination of IgG red blood cell antibodies .

研究了木瓜蛋白酶和胃蛋白酶对草鱼皮的酶促水解，以及水解液通过Maillard反应合成肉类香味料。

The enzymatic hydrolysis of grass carp skin using papain and pepsin were studied respectively . Furthermore , the synthesis of meat flavor aroma of enzymatic hydrolysates through Maillard reaction was studied .

对木瓜蛋白酶用量、处理温度、.处理时间进行试验，并且通过L9（33）正交试验，筛选出木瓜蛋白酶嫩化骆驼肉的最佳条件。

Experiments were conducted with papain dosage , treatment temperature , treatment time . L9 ( 33 ) orthogonal experiment was used to sift the optimal condition of the tenderization .

方法：分别于实验第1、4日，向27只家兔（雌雄不限）右膝关节内注射1.6%木瓜蛋白酶溶液0.3ml，建立兔膝骨关节炎模型。

Methods : Animal models of osteoarthritis in knee of rabbits had been established by intraarticular injection of 1.6 % papain solution .

采用盐析法提取米糠蛋白，分别选用胃蛋白酶、胰蛋白酶、木瓜蛋白酶等单独或联合水解米糠蛋白，以从酶解米糠蛋白中分离获得具有血管紧张素转换酶（ACE）抑制活性的短肽组分。

To obtain angiotensin converting enzyme ( ACE ) inhibitor from protease-digested rice bran protein , the method of salt-out was adopted to extract rice bran protein , which was then digested by pepsin , trypsin , papain separately or jointly .

采用木瓜蛋白酶对绿豆蛋白进行水解，确定了水解绿豆蛋白制备绿豆多肽的最佳水解条件为最适pH为6.5，底物浓度7%，酶浓度为底物的8%，水解温度45℃，水解时间为4h。

Papain was used to hydrolyze mung bean protein . The optimum condition for papain to hydrolyze mung bean protein was pH 6.5 , the substrate concentration 7 % , the enzymatic dosage 8 % , hydrolyzation temperature 45 ℃ and hydrolyzation time 4 h.