次氮基三乙酸

次氮基三乙酸次氮基三乙酸
  1. 经镍-次氮基三乙酸(NiNTA)金属亲合层析方法对表达产物纯化后,用3H标记底物的方法测定了重组强、弱毒株dUTPase的活性。

    The recombinant proteins were purified with nickel-nitrilotriacetic acid ( Ni-NTA ) metal-affinity chromatography and identified by means of isotopic assay ( tritium-labeled substrate ) .