次级卵母细胞

用注射LH后25-35小时的次级卵母细胞作胞质供体均可获得较高的卵核移植胚体外发育率；

Higher survival rate of nuclear transplanted embryos in vitro can be obtained when secondary oocytes collected at 25-35 h after LH injection were used as cytoplast donors ;

受精膜举起后，由次级卵母细胞排放出第二极体。

After fertilization membrane was lifted , the secondary egg emitted the second polar body into the perivitelline space .

在显微操作仪的控制下，将分离后的单个卵裂球注入去核次级卵母细胞的卵周隙。

The blastomeres separated from the embryos were injected into the perivitelline space of enucleated secondary oocytes under the control of micromanipulator .

由山羊4-32细胞期胚胎的卵裂球和去核次级卵母细胞构成的卵核移植胚在体内仍可发育为正常个体；

Nuclear transplanted embryos produced by transferring blastomeres from 4-32 cell embryos into enucleated secondary oocytes can develop into normal kids in vivo ;

在次级卵泡，卵母细胞和卵泡膜内侧的颗粒细胞内有大量锌离子分布。

In the secondary ovarian follicle , large amount of zinc ions were discovered in oocyte and granular cells . 5 .