比活

纯化倍数达720倍，比活840u·mg－1蛋白。

Specific activity was 840 u · mg - 1 protein .

所得SOD酶比活3048μ／mg，对粗酶液的收率73%，纯化倍数96倍。

The yield from crude enzyme liquor was 73 % , . The purification multiple was 96-fold as such .

结果表明，采用透析复性法，rPA（K）的比活可达1.33×10~5IU/mg；

Through dialysis renaturation , the specific activity was 1.33 × 10 ~ 5 IU / mg ;

纯化的酶比活可达13500μ/mg，经PAGE、SDS-PAGE和快速蛋白液相色谱（FPLC）检测，结果表明，纯化酶是均一的。

The purified enzyme is found to be homogeneous by the analysis of SDS-PAGE , PAGE and FPLC .

以反应初速度计，其比活达4.1moleC2H4/分·moleMo，约为固氮酶活性的1%。

Its specific activity is 4.1 mole C_2H_4 / min · mole Mo which is about 1 % of the activity of natural nitrogenase .

结果包涵体蛋白可以部分复性，比活达到900Umgprotein。

Result The inclusion body proteins could be partially renatured , its specific activity was about 900U / mg protein .

并研究了pH、DO、NH2－N、葡萄糖酸、细胞产率，酶比活及体积产酶速率等参数随比生长速率变化的规律。

The relationship between specific growth rate and DO , NH 2 N , gluconic acid , biomass productivity , specific enzyme activity , enzyme volumetric productivity were studied .

然后添加硫酸铵至饱和浓度90%进行盐析，SOD冻干粉的比活为168.3U/mg（蛋白）。

Ammonium sulfate was added with stirring to SOD extracts to make a 90 % saturated solution , and SOD powder was gained by lyophilization , whose specific activities were 168.3 U / mg protein .

采用渗透压冲击法提取青霉素酰化酶，粗酶液比活达8.20×10-8mol/（s·mg），比超声波法高10倍以上，酶活总收率达93.3%。

Penicillin acylase was extracted by osmotic shock method . The specific activity of crude extract was 8.2 × 10-8 mol / ( s · mg ), it was ten-times higher than that from ultrasonic method . The total recovery was 93.3 % .

在优化条件下，1L发酵液可得50000单位的聚合酶，比活可达10000U/mg蛋白质。

Then 50,000 unit of polymerase was obtained from 1L culture , with a specific activity of 10,000U / mg .

提纯的GTF抗原，平均比活，提纯倍数和收率，分别为58.34u／mg、6.08倍和19.25%。

The specific activity , purification fold and recovery of purifies GTF antigen were 58.34U / mg , 6.08 fold and 19.25 % respectively .

随着激发子的逐步纯化，其诱导不同水稻品系叶片中的POD比活相应显著升高（P<0.01），CSBⅠ的诱导活性最高。

With the further purification , the induction of the peroxidase activity in different rice cultivar leaves after treatment with each step elicitor was higher ( P < 0.01 ) .

经碱溶-酸沉-二次盐析得到的LOX的酶比活最高，达到9.42×107U/g酶粉。

Specific activity of LOX 9.42 × 107 ( U / g )( powdered enzyme ) obtained by alkali-dissolving and acid-leaching followed by two salting-out steps is the highest of all .

收集下相，经过超滤浓缩等一系列步骤后，比活达到了308.8U/mg，提纯近455倍。

Purification folds was 455 and specific activity was 308.8U/mg .

以壳聚糖为基质的金属螯合亲和层析法纯化大蒜SOD，得到比活为1191U/mg的酶蛋白，回收率为71%，纯化倍数为7.29。

Superoxide Dismutase ( SOD ) from garlic was purified with the metal chelate affinity chromatography ( MCAC ), using chitosan as substrate . The SOD was purified to homogeneous in one step affinity chromatography . Its specific activity was 1191U / mg and recover was 71 % .

通过采用盐析和透析等提纯方法，对解脂假丝酵母（Candidalipolytica）脂肪酶进行了纯化，比活提高了2.69倍，回收率45%。

By means of salting out and dialysis , the lipase from Candida lipolytica was purified with the results of the specific activity 2.69 times higher and the activity recovery of 45 % .

初始培养基中加入10g/L的葡萄糖酸钙，能够诱导葡萄糖酸激酶的生成，大幅提高其比活，增大磷酸戊糖（HMP）途径的通量。

This may be ascribed to the induction of glucose kinase synthesis , leading to the improvement of hexose monophosphate ( HMP ) shunt flux and its specific activity greatly .

最后用自制的人血浆糖蛋白一Sepharose4B亲合层析柱可将上述MML粗制品的比活再提高400多倍。

Finally by using our self-made human plasma glycoprotein-Sepharose 4B affinity colum chromatography , the MML in the primary MML fluid was highly purified .

报道了以刀豆为原料制备脲酶的一种简便方法，其总收率为37.1%，比活达43.6μmol·min~（-1）/mg蛋白。

The present paper covers a simple method for preparing urease from jack bean . In terms of the method , the total yield was 37 . 1 % and the specific activity was 43 . 6 μ mol ? min-1 / mg protein .

CHS菌株发酵液经离心、硫酸铵盐析、透析、离子交换层析和分子筛层析后，酶的比活由59.4U/mg提高到860.8U/mg，提高了14.5倍，回收率为3.24%。

The alkaline protease from strain CHS was purified from the culture supernatant by centrifugation , homogeneity by ammonium sulfate precipitation , dialysis , ion-exchange chromatography and gel filtration chromatography . The specific activity of the enzyme was raised from 59.4U . mg-1 to 860.8U .

经体外变复性，Zn2＋选择性沉淀，抗体亲和柱层析，及Benzamidine亲和吸附，所表达的人尿激酶原被纯化为单一条带，其比活约110000IU／mg。

After denaturation and renaturation in vitro , the expressed pro UK was purified to Identity by Zn 2 + selective precipitation , immuno affinity chromato graphy , and Benzamidine affinity adsorption . The specific activity of the purified human pro UK was about 110000IU / mg .

葡聚糖凝胶G100过滤分离出三个活力峰，用SDS-聚丙烯酰胺凝胶电泳显示其分子量分别为6000053000和32000道尔顿，其中53000组分比活最高，达每毫克蛋白3135活力单位。

Three active fractions were isolated , and the molecular weights were estimated to be about 60000 , 53000 and 32000 Daltons respectively by SDS-polyacrylamide gel electrophoresis . The specific activity of 53000 fraction was found to be 3135u / mg protein .

淀粉废水水解酸化处理中的酶比活变化特性

Changes of Specific Enzyme Activity in Hydrolytic Acidification Treatment of Starch Wastewater

超滤法和盐析法粗提基因工程干扰素其比活的比较

Comparison of Specific Activities of Recombinant Interferon Extraced by Ultrafiltration and Salt out

纯化了近690倍，比活高达5900单位/毫克蛋白。

It has been purified about 690 - fold .

使枯木比活嘴唇更值得艳羡。

Making dead wood more blest than living lips .

该工艺简单、成本低、效价和比活高。

The process is simple , low cost , high recovery and specific activity .

用纤维蛋白平板法测得重组蛋白的比活为92000IU/mg。

The special activity of the chimera was 92 000 IU / mg detected by fibrin plate determination .

近年来生物吸附重金属的研究取得了很大进展，很多研究发现非活性微生物也可以吸附重金属离子，甚至比活菌的吸附效率更高。

It was found that inactive microorganisms can absorb heavy metal ions , even better than living cells .

每公斤猪胰脏可得比活为30.69u/mg的弹性酶7.03g。

The average yield and activity of elastase were 7.03 g / kg pancrease and 30.69 u / mg , respectively .