点样

DNA芯片的制备方法大致可以分为点样法和在片合成法。

Most of the low-density DNA chips are prepared by spotting , and high-density DNA chips in situ synthesis .

通过研究不同溶剂来溶解PCR产物后，点样对杂交信号的影响，使基因芯片的技术环节得到优化。

By researching how spotting affect the hybridizing signal after dissolving PCR products with different impregnant , we hope to optimize the technology of microarray .

方法针对HDV基因保守区域设计多对PCR引物。用芯片点样仪将PCR产物点到玻片上制成基因芯片。

Method Several pairs of specific PCR primers were designed according to the conserved region of HDV genome .

薄层色谱ABC（2）&薄层板制备和点样

ABC of Thin-Layer Chromatography ── II : Preparation of Thin Layer Plates and Sample Application

结论：BAC克隆作为探针点样制备CGH微阵列时，FISH法鉴定是必要的。

Conclusion FISH technique is effective and useful in the identification of BAC clones for array CGH .

确定鸡抗EDS-76抗体和羊抗兔抗体的合适点样量为1μg。

The optimal amount of chicken anti-EDS-76 virus and sheep anti-rabbit IgG was determined as 1 μ g.

N（≠2~p）点样值的时间信号函数的W-H变换

The Walsh-Hadamard Transform Of The Time Signal Function With N (≠ 2 ~ P ) Samples

首先进行芯片杂交动力学研究，考察信号值和Ratio值与PCR产物点样浓度、PCR产物长度和荧光标记探针长度之间的关系，探讨这三者对SNP分型的影响；

We first studied the hybridization dynamics to optimize the conditions of this method by observing how spotting concentration of PCR products , PCR products length and fluorescence labeled probe length affect genotyping ;

按设计模式，以自动化微阵列点样仪将探针点样于APTESPDC修饰的玻片表面。

The probes were spotted on the surface of APTES-PDC-modified glass slides by microarray spotter tracing the designed patterns .

结果黄芪甲苷在0.4～2μg范围内，点样量与峰面积呈良好的线性关系，平均回收率为99.98%，RSD为2.18%。

Results Methods of TLC are simple and reliable , a good linearity was shown at the concentration of 0.4 ~ 2 μ g of Astragaloside I , the average recovery was 99.98 % and the RSD was 2.18 % .

以硅胶或氧化铝吸附剂涂制于TLC烧结棒上，然后点样色谱展开，烧结棒以恒定速度通过检测器火焰，各组分被自动扫描。

TLC sintered bar is coated with silica gel or alumina as absorbent . After repeated chromatographic developments , the bar moves through flame detector at a constant rate , and each component is automatically scanned .

在利用TLC法检测某水溶性染料的合成过程中，用细玻璃棒代替毛细管点样，降低薄层展开高度，收到了良好的效果。

A thin glass stick instead of capillary tube was used for the test of the synthesis process of a water-soluble dye by TLC , and the height of TLC was shortened , good results were achieved .

在试验所用的低频范围内（1～10Hz），点样系统能够对每个驱动脉冲作出响应，具有数字可控性。

The spotting system responds to each driving impulse ( 1Hz ~ 10Hz ) and can be controlled digitally .

结果：TLC方法简便，可靠；苦参碱在1.167～5.835μg范围内，点样量与峰面积积分值呈良好的线性关系，平均回收率为97.82%，RSD为1.27%。

Results : Methods of TLC are simple and reliable , a good linearity was shown at the concentration of 1.167 μ g to 5.835 μ g of matrine , the average recovery was 97.82 % and the RSD was 1.27 % .

点样基片经室温干燥2h、水合处理10s、紫外线交联25min和0.2%SDS洗涤5min等系列处理后，成功制备出检测基因芯片。

The glass slide spotted target genes was dried at room temperature for 2 hours , hydrated for 10 seconds , lighted with UV for 25 mins and washed with 0.2 % SDS soulution for 5 mins .

结果：8例志愿者16个骶髂关节的T1WI和T2WI可直接显示正常骶髂关节软骨，表现为线形或点样的中等信号影。

Results : MR imaging directly showed the cartilage in the synovial compartment as a thin linear or dot zone of intermediate signal intensity on both T 1 and T 2 weighted images in all 16 sacroiliac ( SI ) joints of the 8 volunteers .

在无菌环境下点样于内径为6mm的不锈钢管内，观察含菌琼脂平皿内抑菌圈大小。

In the bacteria - free environment , we dropped the above four solutions as a sample into the four different stainless steel tubes with an inner diameter of 6 mm and observed the size of the inhibitory rings in the bacterial agar plane pools .

点样位置对血清蛋白醋酸纤维薄膜电泳行为的改变

Altered Electrophoresis of Serum Proteins by Its Position on Acetyl Cellulose

你点样可显示出自己既责任感？

How do you show your responsibility to your work ?

将探针按一定的顺序点样于醛基化的玻片上，制备成基因芯片。

Gene chips were made by doting the probes to modified slide .

非接触式点样法是制作基因芯片的一项重要技术。

Noncontact printing is one of the important technologies to make microarrays .

使用生物芯片点样仪，制成预先设计好的微阵列。

Spot microarray as designed by the spotting machine .

该系统的运动驱动程序是保证点样精度的关键部分。

The motion driven program is the key part to guarantee pointing accurate .

咁，男人点样先揾到个女人系隔离？

Then , how can men find their women ?

点样量影响荧光信号的强度。

The concentration of protein in the spotting solution significantly affected the fluorescence intensity .

点样条件包括点样量（或体积）、速度、带宽等；

The qualifications of application involved the amount , speed and bandwidth of application .

接触式点样机器人及相关技术研究进展

Development of Contact Printing Arrayer and Related Technologies

同时建议对原料有关物质检查项中杂质对照的点样方法进行修改。

We suggest the amendment of dot method in examination of rs in cefradine .

没准我们还能稍稍换点样

maybe we 'd have a little variety .

生物芯片点样针性能指标实验研究

Study on Characteristics of Micro Spotting Pin