点样
- 网络spot;point sample;printing;spot sample
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DNA芯片的制备方法大致可以分为点样法和在片合成法。
Most of the low-density DNA chips are prepared by spotting , and high-density DNA chips in situ synthesis .
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通过研究不同溶剂来溶解PCR产物后,点样对杂交信号的影响,使基因芯片的技术环节得到优化。
By researching how spotting affect the hybridizing signal after dissolving PCR products with different impregnant , we hope to optimize the technology of microarray .
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方法针对HDV基因保守区域设计多对PCR引物。用芯片点样仪将PCR产物点到玻片上制成基因芯片。
Method Several pairs of specific PCR primers were designed according to the conserved region of HDV genome .
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薄层色谱ABC(2)&薄层板制备和点样
ABC of Thin-Layer Chromatography ── II : Preparation of Thin Layer Plates and Sample Application
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结论:BAC克隆作为探针点样制备CGH微阵列时,FISH法鉴定是必要的。
Conclusion FISH technique is effective and useful in the identification of BAC clones for array CGH .
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确定鸡抗EDS-76抗体和羊抗兔抗体的合适点样量为1μg。
The optimal amount of chicken anti-EDS-76 virus and sheep anti-rabbit IgG was determined as 1 μ g.
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N(≠2~p)点样值的时间信号函数的W-H变换
The Walsh-Hadamard Transform Of The Time Signal Function With N (≠ 2 ~ P ) Samples
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首先进行芯片杂交动力学研究,考察信号值和Ratio值与PCR产物点样浓度、PCR产物长度和荧光标记探针长度之间的关系,探讨这三者对SNP分型的影响;
We first studied the hybridization dynamics to optimize the conditions of this method by observing how spotting concentration of PCR products , PCR products length and fluorescence labeled probe length affect genotyping ;
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按设计模式,以自动化微阵列点样仪将探针点样于APTESPDC修饰的玻片表面。
The probes were spotted on the surface of APTES-PDC-modified glass slides by microarray spotter tracing the designed patterns .
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结果黄芪甲苷在0.4~2μg范围内,点样量与峰面积呈良好的线性关系,平均回收率为99.98%,RSD为2.18%。
Results Methods of TLC are simple and reliable , a good linearity was shown at the concentration of 0.4 ~ 2 μ g of Astragaloside I , the average recovery was 99.98 % and the RSD was 2.18 % .
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以硅胶或氧化铝吸附剂涂制于TLC烧结棒上,然后点样色谱展开,烧结棒以恒定速度通过检测器火焰,各组分被自动扫描。
TLC sintered bar is coated with silica gel or alumina as absorbent . After repeated chromatographic developments , the bar moves through flame detector at a constant rate , and each component is automatically scanned .
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在利用TLC法检测某水溶性染料的合成过程中,用细玻璃棒代替毛细管点样,降低薄层展开高度,收到了良好的效果。
A thin glass stick instead of capillary tube was used for the test of the synthesis process of a water-soluble dye by TLC , and the height of TLC was shortened , good results were achieved .
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在试验所用的低频范围内(1~10Hz),点样系统能够对每个驱动脉冲作出响应,具有数字可控性。
The spotting system responds to each driving impulse ( 1Hz ~ 10Hz ) and can be controlled digitally .
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结果:TLC方法简便,可靠;苦参碱在1.167~5.835μg范围内,点样量与峰面积积分值呈良好的线性关系,平均回收率为97.82%,RSD为1.27%。
Results : Methods of TLC are simple and reliable , a good linearity was shown at the concentration of 1.167 μ g to 5.835 μ g of matrine , the average recovery was 97.82 % and the RSD was 1.27 % .
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点样基片经室温干燥2h、水合处理10s、紫外线交联25min和0.2%SDS洗涤5min等系列处理后,成功制备出检测基因芯片。
The glass slide spotted target genes was dried at room temperature for 2 hours , hydrated for 10 seconds , lighted with UV for 25 mins and washed with 0.2 % SDS soulution for 5 mins .
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结果:8例志愿者16个骶髂关节的T1WI和T2WI可直接显示正常骶髂关节软骨,表现为线形或点样的中等信号影。
Results : MR imaging directly showed the cartilage in the synovial compartment as a thin linear or dot zone of intermediate signal intensity on both T 1 and T 2 weighted images in all 16 sacroiliac ( SI ) joints of the 8 volunteers .
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在无菌环境下点样于内径为6mm的不锈钢管内,观察含菌琼脂平皿内抑菌圈大小。
In the bacteria - free environment , we dropped the above four solutions as a sample into the four different stainless steel tubes with an inner diameter of 6 mm and observed the size of the inhibitory rings in the bacterial agar plane pools .
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点样位置对血清蛋白醋酸纤维薄膜电泳行为的改变
Altered Electrophoresis of Serum Proteins by Its Position on Acetyl Cellulose
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你点样可显示出自己既责任感?
How do you show your responsibility to your work ?
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将探针按一定的顺序点样于醛基化的玻片上,制备成基因芯片。
Gene chips were made by doting the probes to modified slide .
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非接触式点样法是制作基因芯片的一项重要技术。
Noncontact printing is one of the important technologies to make microarrays .
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使用生物芯片点样仪,制成预先设计好的微阵列。
Spot microarray as designed by the spotting machine .
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该系统的运动驱动程序是保证点样精度的关键部分。
The motion driven program is the key part to guarantee pointing accurate .
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咁,男人点样先揾到个女人系隔离?
Then , how can men find their women ?
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点样量影响荧光信号的强度。
The concentration of protein in the spotting solution significantly affected the fluorescence intensity .
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点样条件包括点样量(或体积)、速度、带宽等;
The qualifications of application involved the amount , speed and bandwidth of application .
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接触式点样机器人及相关技术研究进展
Development of Contact Printing Arrayer and Related Technologies
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同时建议对原料有关物质检查项中杂质对照的点样方法进行修改。
We suggest the amendment of dot method in examination of rs in cefradine .
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没准我们还能稍稍换点样
maybe we 'd have a little variety .
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生物芯片点样针性能指标实验研究
Study on Characteristics of Micro Spotting Pin