真核系统

实验表明，可以利用原核系统取代真核系统表达PrP，并用生物素标记取代35S标记纯化蛋白，建立一个更加安全方便的无细胞构象转化系统，用于朊病毒的研究。

Our study supplies a new system , using more efficiently prokaryotic expressed PrP instead of that expressed in mammalian or insect cells and more easily handling and environmental protecting biotin instead of isotope in cell free conversion for prion study .

目的在真核系统表达XHFVS基因片段，制备安全、特异、便于操作的S蛋白。

Objectives Expressing of Xinjiang hemorrhagic fever virus ( XHFV ) S gene in an eukaryote expression system and getting a safe , specific and easy manipulating antigen .

猪圆环病毒2型核衣壳蛋白基因在原核和真核系统中的表达

Expression of Nucleocapsid Gene of Porcine Circovirus Type 2 in Prokaryote and Eukaryote System

在该项研究中将水蛭素基因转入酵母系统中进行表达，以评价人工合成基因在真核系统中表达的效率和探讨影响表达水平的因素。

In this research the hirudin gene was synthesized and put into yeast system for expression . The efficiency of gene expression was valued and some factors affecting it were probed .

人尿激酶原cDNA在昆虫杆状病毒真核表达系统中的高效表达

Expression of The Human Pro-Urokinase cDNA Product in Insect Cells Using Baculovirus System

Notch配体表达系统分真核表达系统和原核表达系统。

And Notch ligands expression system can classify into eukaryotic and prokaryotic expression systems .

由于此HA蛋白是原核表达产物，回避了真核表达系统复杂的操作过程和昂贵的费用，所以，这个麻疹病毒血凝素基因工程抗原有望成为一种新型、便捷的麻疹病毒诊断试剂

The results showed that the MV hemagglutinin gene engineering antigen might provide a more efficient diagnostic reagent for detection of the MV infection

结论建立了两个高效表达突变CD59的真核表达系统，获得阳性克隆细胞株。

Conclusion A eukaryotic system that expressing mutant CD59 cDNA was successfully set up .

目的：构建淋病奈瑟菌孔蛋白PIA（PorinIA）基因的真核表达系统，为淋病DNA疫苗的研究提供材料。

Objective : To construct an eukaryotic expression system carrying the Neisseria gonorrhoeae Porin IA ( PIA ) gene .

目的构建人谷胱甘肽硫转移酶A1真核表达系统，为毒理学、遗传药理学的研究等提供材料。

Objective To construct an eukaryotic expression vector carrying human glutathione-S-transferase ( GST ) A1gene and to provide study materials for toxicology and pharmacogenetic .

芥菜SVP与FLC能够在pET原核表达系统和酵母真核表达系统中相互作用，并可以形成稳定的异源蛋白复合物。

The protein interaction between mustard FLC and SVP were screened via pET prokaryotic expression system and yeast two-hybrid system they formed stable heterogeny protein complex and delay mustard flowering .

结论成功构建了hIL-10真核表达系统，为今后的临床研究及基因工程药物生产打下基础。

Conclusion The eukaryotic expression system of hIL-10 was successfully established , which might provide the basis for studying affection of IL-10 in clinics and production of gene engineering medicine .

结论成功构建的IA2真核表达系统对于T1DM基因疫苗预防策略和对于IA2生理功能的研究都具有重要的意义。

Conclusion The pEGFP / IA-2 is successfully constructed , which is the first step to prepare type 1 diabetes gene vaccine and has an important role in the prevention of type 1 diabetes and the study of biological function of IA-2 .

四环素调控的真核表达系统是生物学研究中的一个重要的工具。

Tetracycline-regulated eukaryotic expression system is a useful tool in area of biological researches .

痘苗病毒真核表达系统可以高效表达目的蛋白。

CB could be expressed in high yield by vaccinia virus eukaryotic expression system .

精确预测剪接位点是真核基因系统研究的第一步。

Accurate prediction for splice sites is the primary step in the system research for eukaryotic genes .

而酵母表达系统是一种真核表达系统，其所表达的蛋白产物修饰合理，活性高。

The yeast expression system is eukaryotic and the protein expressed is modified and has high activity .

真核表达系统的研究进展

Advances in Eukaryotic Expression Systems

膜蛋白因受其自身性质所限，大量表达会对细胞产生毒性作用，且在真核表达系统中低表达。

The mass expression of membrane proteins would bring toxic effect to cells and it only shows low expression in eukaryotic expression systems .

目前应用的外源蛋白表达系统有原核表达系统、真核表达系统等。

There are many systems used for heterologous protein expression at present , including prokaryotic expression system , eukaryotic expression system et al .

基因工程中常用的真核表达系统有：酵母表达系统、昆虫细胞表达系统及哺乳动物细胞表达系统。

At present , There mainly are three expression systems including yeast expression system , insect cell expression system and mammalian cell expression system .

同时杆状病毒-昆虫（虫体）表达系统是最近发展起来的高效表达外源基因的真核表达系统。

The baculovirus-insects expression system is one of the promising eucaryotic systems in which a lot of foreign genes of economic value have been efficiently expressed .

杆状病毒昆虫表达系统是常用真核表达系统之一，是蛋白结构和功能研究的发展趋势。

Insect baculovirus expression system is the one of the commonly used eukaryotic expression systems , which is the trend of structural and functional research of proteins .

杆状病毒昆虫细胞表达系统是逐渐发展起来，并且在现阶段得到广泛应用的真核表达系统，它具有蛋白翻译后加工修饰的功能，从而能够形成与天然蛋白高度一致并且可溶的蛋白。

Baculovirus expression vector system is an eukaryotic expression system which was gradually developed , and widely used . It has the function of modification , thus forming the protein highly similar to the natural and soluble .

酵母是一个近年来被广泛使用的真核表达系统，应用信号肽序列构建酵母分泌表达系统，即可方便纯化操作，又可提高外源蛋白的分泌表达效率。

Yeast is a kind of eukaryotic expression system , which has been widely used in recent years . It is not only convenient to purification , but also can promote the secretion and expression efficacy of foreign protein by using signal peptide sequence to construct yeast secretory expression system .

基于杆状病毒F蛋白的真核表面展示系统的构建

Construction of Eukaryotic Surface Display Based on the Baculoviral F Protein

酿酒酵母（saccharomycescerevisiae）是一种理想的真核蛋白表达系统。

Saccharomyces cerevisiae was a desired expression system for eukaryotic protein .

真核细胞转录系统启动T7启动子起始的研究

Transcriptional initiation of T7 promoter by transcriptional system in eukaryotic cells

结果成功构建人突变CD59的真核细胞表达系统；运用各种免疫学技术检测获得CD59高表达细胞。

Results A eukaryotic expression system for high expression human mutant CD59 were successfully set up , and stable high expression CHO cell lines obtained .

巴斯德毕赤酵母（Pichiapastoris）一个真核高效表达系统，已经有400多种基因工程产品在该体系上表达，如：肿瘤坏死因子，重组人血清白蛋白等。

Pichia pastoris is a new efficient eukaryotic expression system in recent years . It was reported that more than 400 genetic engineering products have been expressed in the system , such as : human interleukin-2 , tumor necrosis factor , recombinant human serum albumin .