真叶

生理生化测定结果表明，经BABA诱导处理黄瓜第一叶真叶后，提高新生真叶的PAL和POD的活性；

Physiologicaland biochemical measurements indicated that BABA treatment of the first true leaf of cucumber caused an increase ofPAL and POD activity in subsequently emerging leaves .

结果表明，番茄苗期TMV、叶霉病、根结线虫病的最佳复合鉴定方法是，在2片真叶接种根结线虫，采用灌根法，接种浓度5000条/株，接种后50d调查病情；

Leaf Mold and Root-knot Nematode is : Root-knot Nematode is inoculated at 2 true leaf , water into the soil , 5 000 nematodes / plant , investigate the disease after 50 days ;

生理生化指标3个：叶绿素A／B比值、真叶可溶性蛋白含量、真叶POD活性。

Physiological-biochemical indexes : the chlorophyll a / b , the content of dissolubility protein and POD activity .

在KT+NAA的B5培养基上，上胚轴较小真叶外植体成苗率高。

However , in the B_5 medium which contains KT + NAA , the rate of plantlets developed from epicotyl is higher than that from true leaves .

局部诱导（第一片真叶）的POD活性第6天达到最大，系统诱导（第二片真叶）的POD活性高峰出现在第10天；

POD activity reached maximum after 6 and 10 days of inoculation in the first and second leaves , respectively .

利用50mg/ml的牛蒡寡糖诱导黄瓜幼苗第1真叶3d后接种白粉病菌，调查黄瓜植株的病情指数。

Cucumber seedlings were inoculated with powdery mildew ( Sphaerotheca fuliginea ) in true leaf 1 and leaf 2 after the treatment with the first true leaf with 5.0mg/ml burdock oligosaccharide .

825mW/cm~2的CO2激光照射茄子干种于10秒，13秒，15秒，可以增强幼苗素质，激光组的子叶、真叶的干重，叶面积，叶绿素含量及根系的相对还原力均高于对照。

The quality of seedlings may be enhanced when dry seeds of eggplant were irradiated with 825mW / cm2 of CO2 laser at a time of 10s ; 13s ; 15s .

本文研究了绿豆的种质差异、光照、真叶和下胚轴与BR促进的绿豆上胚轴生长的关系。发现绿豆品系的差异显著地影响上胚轴对BR的响应；

Relationship of strain character , light , leaf and hypocotyl to brassinolide ( BR ) promoted epicotyl growth in mung bean cuttings were studied .

花原基将分化期，诱导72h，2片真叶充分开展；

The would be differentiating stage of floral primodium , after 72 h light treatment which the second leaf was fully expanded ;

在植物生长室中，黄瓜植株第1片真叶出现后，用人工UVB光源照射60d，测定植物各叶位叶片的生长和生理活动。

The growth and physiological variation of cucumber grown under UV B radiation for 60 d in plant growth chamber were monitored .

甜瓜品种‘GT-1’真叶再生体系的建立

Establishment of regeneration system of leaf of oriental melon ' GT-1 '

通过水培实验，研究了不同浓度的重金属Cd，Cr，Pb，Zn对小麦的生长初期（发芽、长出两片真叶后7d）的影响。

Using method of water culture plant , researched the effect of heavy metals ( Cd , Cr , Pb , Zn ) on initial stages of growth of wheat ( 7days after the first two leaves had grown ) .

柠条根中PAL活性和比活性以真叶期最高，其茎和叶中PAL活性和比活性以速生期最高。

The PAL activity and specific activity of its root were the highest at the real leaf stage ; the PAL activity and specific activity of its leaf and stem were the highest at rapid growth stage .

大豆小真叶外植体在合有NAA或KT+NAA的B5培养基上，上胚轴外植体在含有KT+NAA的B5培养基上，可脱分化形成愈伤组织，并由愈伤组织直接分化成苗。

The young true leaves explant of soybean are cultured in B_5 basal medium containing NAA or KT + NAA , epicotyls explants are cultured in the B_5 medium containing KT + NAA , they are able to dedifferentiate into callus tissues , then directly develop into plantlets .

3片真叶接种TMV，采用摩擦法接种，接种量1g病叶加蒸留水10mL，接种后第3d复接一次，接种后20d调查病情；

TMV is inoculated at 3 true leaf , wear on the leaf , the concentration is 1 g diseased leaf mingled with 10 g distilled water , investigate the disease after 20 days ;

综合所有试验数值可见，在一至两片真叶展平时施用1、2mM水杨酸处理对盐胁迫的缓解效果较佳，实际生产中应尽量在较小叶龄时施用。

It is concerned that use 1 、 2mM salicylic acid when one or two piece of euphylla the relieve effect is best . we should use SA in lesser age of leaves .

依据增强UVB辐射、NaCl胁迫及其复合处理不同天数，分别测定各处理小麦幼苗第一片真叶的净光合速率、气孔导度、叶肉细胞间隙二氧化碳浓度、叶绿素含量、黄酮含量和苯丙氨酸裂解酶活性。

During increased UV-B radiation , NaCl strees and their combined treatment , measuring the net photosynthetic rate , stomatal conductance , intercellular CO_2 concentration , PAL activity , the contents of chlorophyll and flavone of first leave of wheat seedlings respectively .

本文报道激动素（KT）和萘乙酸（NAA）不同浓度配比对大豆上胚轴和小真叶外植体愈伤组织器官分化的影响以及对分化成苗与未分化愈伤组织过氧化物酶活性的分析。

The effect of various concentrations and ratio of kinetin and NAA on organ differentiation of epicotyls and callus of explants of young uni-foliolate leaves , and the activity of peroxidase of the differentiated plantlets and undifferentiated callus was studied in thig paper .

番茄TMV、枯萎病、根结线虫病最佳鉴定方法是：2片真叶接种根结线虫，3片真叶接种TMV，4片真叶接种枯萎病。

The best method of identification of TMV - . Fusarium Wilt and Rot-knot Nematode is : Rot-knot Nematode is inoculated at 2 true leaf , TMV is inoculated at 3 true leaf , and Fusarium Wilt is inoculated at 4 true leaf .

真叶期机械损伤处理后第15d起黄瓜植株的株高、茎粗、最大叶叶面积和叶数与对照达到了显著、极显著差异。

After mechanical wounding treatment 15d at stage of the first true leaf , the height 、 the width 、 the largest leaf area and leaf number with the control were reached a significant , very significant differences . 2 .

体细胞胚成熟以后转到含0.5%活性炭的无激素1/2MS基本培养基上约40d后可长出1.5～2.5cm的根，约60d后可长出真叶。

The mature somatic embryo developed root of 1.5 to 2.5 cm in length if cultured on 1 / 2 MS medium supplemented with 0.5 % activated charcoal but without hormone for 40 d and leaves for 60 d.

结果表明，BTH及其结合挑战接种处理后，第二片真叶的POD活性分别提高了4.34倍和9.96倍，CHT活性提高11.17倍和8.93倍，且木质素积累有所增加。

The results showed that POD activity of second leaves increased 4.34 times and 9.96 times respectively , CHT activity increased 11.17 times and 8.93 times and accumulation of lignin was increase too , after the first leaves were treated with BTH and BTH with challenge inoculation respectively .

番茄品种白果强丰经盐胁迫后其真叶中分子量约为14KD和17KD蛋白质减少，同时诱导出一条分子量约为22KD的新带。

On salt treatment , about 14 KD and 17 KD proteins which present in the leaves of tomato decreased meanwhile a new protein band about 22 KD was induced .

结果表明：SA未处理的菜豆幼苗不同器官中均存在抗氰呼吸，其中真叶的Valt和ρValt最高，其次是子叶，而下胚轴的最低；

The results demonstrate that the cyanide-resistant respiration exists in different organs of bean seedlings , and the ρ V_ ( alt ) in true leaf was the highest , that in cotyledon the next , and hypocotyl ρ V_ ( alt ) was the lowest .

研究了影响农杆菌介导番茄Micro-Tom的遗传转化的几个因素，结果表明：以子叶作为外植体，其转化后再生频率（76.0%）明显高于下胚轴（19.9%）和真叶（28.7%）；

Several factors influencing transformation frequency of Micro-Tom were investigated . The results showed that , when using cotyledon as explant the highest regeneration frequency after transformation could be obtained ( 76.0 % ), compared to leaf ( 28.7 % ), and hypocotyl ( 19.9 % );

真叶和根内的木质素和单宁含量显著和极显著地增加。

The content of lignin and tannin significantly increased .

番茄真叶愈伤组织诱导及植株再生研究

Callus Formation and Plantlet Regeneration from Leaf of Tomato

棉花苗期真叶具有叶调位性能。

A leaf of cotton has the capability of heliotropic movement during seedling stage .

幼苗长出第二片真叶时根部出现根瘤。

Root nodule appears on roots as soon as seedlings have the second real leaf .

光周期对扁豆真叶内源激素及游离氨基酸含量的影响

Effects of Photoperiodic Treatment on the Endogenous Hormones and Dissociative Amino Acid of Hyacinth Bean Leaves