离心法

常常是用离心法分段收集沉淀的

Precipitates are usually collected by centrifugation at intervals .

结论：二次离心法制作的PRP其血小板浓度和血小板回收率显著高于一次离心法。

CONCLUSION : Concentration and recovery rate of platelet in PRP after twice centrifugation are higher than that after once centrifugation .

离心法浓缩~（12）C试验研究

Experimental study on enriching ~ ( 12 ) c by Centrifuge method

快速密度梯度离心法纯化质粒DNA

Purification of plasmid DNA by rapid density gradient centrifugation

方法：大鼠经皮下注射Iso共10天以制备心肌纤维化模型，心肌线粒体以差速离心法分离。对照组仅注射无血清的培养液。

The control group was the model rats received only DMEM injection .

方法细胞：采用密度梯度离心法，分离人脐血单个核细胞（MononuclearCells，MNCs）。分组：分为3组。

Method Human umbilical blood mononuclear cells ( MNCs ) were separated with density gradient centrifugation .

组装成胶晶模板采用自然沉降法和离心法两种方法。所得到的胶晶都具有面心立方结构（fcc）特征，从胶晶规整性比较，自然沉降法优于离心法。

Colloidal crystal template was assembled by a natural sedimentation and a centrifugation method .

密度梯度离心法获得大鼠MSCs，传代培养并扩增。

MSCs were obtained from SD rats using the method of density gradient centrifugation culture .

应用差速离心法提取脑皮质线粒体，Clark氧电极法测定线粒体呼吸功能，及细胞色素C氧化酶（COX）的活性。

Mitochondrial respiratory function and COX activity were measured by Clark oxygen electrode . Mitochondria were obtained by differential centrifugation .

结果：Percoll密度梯度离心法培养可获得均一的MSCs；

RESULTS : Homogeneous MSCs were obtained by percoll density gradient centrifugation .

方法：采用多聚阴离子沉淀结合短时密度梯度超速离心法得到纯化的LDL；

Methods : To isolate LDL by using polyanions precipitation and short-time density gradient ultracentrifugation .

方法：选用54只雄性SD大鼠，密度梯度离心法分离骨髓间充质干细胞（MSCs）。

METHODS : Bone marrow MSCs were obtained by density gradient centrifugation from the tibiae of 54 male SD rats .

采用蔗糖梯度离心法分离HL-60细胞的胞膜、胞浆和胞核；

Using sucrose gradient centrifugation to separate the membrane , the cytoplasm and the nuclei of HL-60 cells .

兔血清LDL、VLDL及HDL用密度梯度超速离心法分离得到。

The serum LDL , VLDL and HDL were isolated by the density gradient ultra centrifugation .

梯度离心法分离食管Langerhans细胞方法初探

Preliminary exploration of the method to isolate esophageal Langerhans cells with discontinuous density gradient centrifugation

所有实验组病例和对照组均采取4毫升静脉血，肝素抗凝，密度梯度离心法分离PBMCs。

PBMCs were separated from 4 ml heparinized blood by density gradient centrifugation .

Percoll密度梯度离心法分离大鼠肺Ⅱ型上皮细胞

Isolation of Rat Type ⅱ Alveolar Epithelial Cells by Discontinuous Percoll Gradient Centrifugation

采用Percoll非连续密度梯度离心法纯化大鼠乳鼠心室肌细胞

Enrichment of cardiomyocytes from neonatal rat ventricular tissues by discontinuous percoll gradient centrifugation

目的：通过分析Percoll梯度离心法处理前后精子相关功能指标，评价该法在精子体外处理中的作用。

Objectives : To evaluate the sperm functional parameters with Percoll gradient centrifugation .

同时利用阶梯密度离心法提取脾淋巴细胞，MTT法检测体外淋巴细胞毒性。

Spleen lymphocytes were extracted by gradient density centrifugation . MTT assay was used to detect the specific cytotoxicity of spleen lymphocyte . 4 .

研究方法：采用Percoll密度梯度离心法分离大鼠BMSCs，对所获得的细胞进行体外培养及鉴定。

Methods BMSCs were isolated by percoll solution from rat bone marrow sample , cultured and identified .

通过静置法、离心法和调节pH值等物理法和化学法处理牛乳，结果以静置法，即：4℃放置24h后去上层脂肪最为方便、实用。

The result indicated that static method is the most convenient and practical which cream layer was removed after laying milk in refrigerator for 24h at 4 ?

2次离心法，以200×g力离心10min制备的PRP，血小板浓度为全血的500%左右为最佳。

Twice centrifugation with 200 × g force , 10 minutes and about 500 % concentration was the best .

方法采用机械分离、酶消化和不连续密度梯度离心法分离提取TIL前体细胞；

Methods TIL were obtained through mechanical separation , enzyme digestion and discontinuous density gradient centrifugation .

应用Percoll非连续密度梯度离心法富集人前列腺上皮细胞

Enrichment of Epithelial Cells from Human Benign Prostatic Hyperplasia by Discontinuous Percoll Gradient Centrifugation

Percoll等密度梯度离心法分离利什曼原虫细胞核

Isolation and purification of nuclei of Leishmania gerbilli by Percoll isopycnic density gradients centrifugation

通过对桃基因组DNA几种提取方法所得的DNA质量分析，表明分步离心法是最为理想的提取方法，能满足RAPD及其他分子生物学研究的需要。

Based on the analysis of several isolation methods for genomic DNA of peach , results showed that the step by step-centrifugation method was optimal for RAPD and other molecular biology researches .

结果：1、利用密度梯度离心法和贴壁法均可以成功在体外分离、培养MSC。

Results : 1 、 MSC can be successfully isolated and developed in vitro with density gradientcentrifugation method and sticking wall method .

方法：Percoll间断梯度离心法。

METHODS : Using Percoll discontinuous gradient .

利用密度梯度离心法提取培养狗MSCs并进行传代，每日观察并照相；

Density gradient centrifugation was applied to extract and culture MSCs of the dogs and generated , which was observed and photographed every day .