结蛋白

应用半定量PCR及WESTERNBLOTTING测定骨骼肌卫星细胞中结蛋白基因及结蛋白含量的表达。

Applicating semi-quantitative PCR and Western blotting to measure the expression of desmin gene and desmin by skeletal muscle satellite which are interfered by different concentrations of androgen .

免疫组化鉴定细胞表型：平滑肌α肌动蛋白，波形蛋白，CD31，平滑肌肌球蛋白和结蛋白。

Cell phenotypic identification was achieved by immunochemistry to measure the following : SM a-actin , vimentin , CD31 , SM-myosin and desmin .

结果：复方汉防己治疗组肝纤维化评分明显低于生理盐水对照组，血清HA及结蛋白阳性细胞数明显减少。

Results : The serum hyaluronic acid ( HA ), fibrosis score and desmin positive cells were all decreased in treatment group as compared to control group .

以鸡胗为材料提纯结蛋白，经鉴定后免疫BALB/c小鼠，采用杂交瘤技术建立了能稳定分泌抗结蛋白单抗的杂交瘤细胞株AD1，其染色体中位数为77。AD1单抗属小鼠Igg1亚类；

BALB / c mice were immunized with desmin isolated from chicken gizzard .

结蛋白和CK全部阴性。结论松果体实质肿瘤中明显具有松果体细胞瘤和松果体母细胞瘤两个阶段特点以及具有中间分化、移行特征者应归于中间分化型松果体实质肿瘤；

Conclusions Pineal parenchymal tumors of intermediate differentiation were characterized by a distinctly biphasic pattern , including areas of pineocytoma and pineoblastoma , or with intermediate differentiation and transitional feature .

显微镜下观察4组形态变化，并通过免疫组化鉴别结蛋白和心肌肌钙蛋白I（CardiacTroponinI，cTnI）表达及其出现时间的早晚。

The morphological changes of MSCs in the 4 groups were observed under microscope , and immunohistochemical staining was used to identify the expression of desmin and cardiac troponin I ( cTnI ), and the emergency time .

良性间皮细胞标本组中，VIM和结蛋白阳性表达率分别为84%和91%，而CEA、EMA、MOC-31、钙粘蛋白E显示低表达，阳性表达率分别为13%、16%、9%和6%。

The results of VIM and desmin , in group showed high positivity with 84 % and 91 % , respectively , whereas the results of CEA , EMA , MOC-31 and E-cadherin showed low positivity with 13 % , 16 % , 9 % and 6 % , respectively .

肌源性肿瘤的结蛋白、波型蛋白及肌动蛋白表达特性

The expression on desmin , vimentin and actin in myogenic tumors

结蛋白免疫组织化学：阳性细胞平均达98.01%。

Desmin immunohistochemistry stain : Positive cells reached 98.01 % averagely .

心房和静脉窦则无结蛋白表达。

No desmin expression was found in the atrium and sinus venosus .

实验性早期心肌缺血结蛋白的免疫组织化学研究

Immunohistochemical Study of Desmin in Experimental Early Myocardial Ischemia

梗死心肌骨骼肌卫星细胞移植后心肌细胞结蛋白变化

The Changes of Infarcted Myocardial Desmin by the Skeletal Muscle Satellite Cell Transplantation

高血压大鼠心脏细胞骨架蛋白α-微管蛋白和结蛋白

The α - tubulin and Desmin Protein Expression in the Heart in Hypertensive Rats

离心运动对大鼠骨骼肌结蛋白分布和表达的影响&对骨骼肌损伤机制的研究

Effect of Eccentric Exercise on Distribution and Expression of Desmin in Skeletal Muscle of Rat

抗地高辛抗体的制备结蛋白相关心肌病发病机制的研究

Producing the Anti digoxin Antibody

结蛋白和波形蛋白在先天性心肌发育缺陷的美西螈心肌培养细胞中的表达及其免疫细胞化学研究

Expression of desmin and vimentin in cultured cardiac myocytes of embryonic axolotl with congenital defect of myocardial development

α&平滑肌肌动蛋白与结蛋白鉴别胃肠道平滑肌肿瘤的对比研究

Comparison of The Use of Antibodies to α & Smooth Muscle Actin and Desmin in Gastrointestinal Smooth Muscle Tumors

体外培养的成肌细胞中94%的细胞胞质呈结蛋白抗体染色强阳性。

Immunocytochemical examination showed that the cytoplasm of 94 % of the cultured myoblasts was strongly positive by anti-desmin staining .

目的探讨结蛋白在早期心肌梗死死后诊断的特异性。

Objective To explore the specificity of Dmin ( D m ) in the postmortem diagnosis of early myocardial infarction .

动物经药物治疗后肝组织内结蛋白阳性细胞数均明显低于生理盐水组。

Desmin-positive cell number in liver tissue in two treated groups was much lower than that in normal saline one .

结蛋白和波形蛋白在运动性肌肉损伤和再生过程中表达及意义的实验研究

An Experimental Study of Expression of Desmin and Vimentin in the Pathological Progress of Exercise-Induced Skeletal Muscle Damage and Regeneration

目的探讨骨髓细胞梗死心肌移植对心肌细胞结蛋白表达的影响。

Objective To study the effect of implantation of bone cells to infarcted myocardium on the expression of myocardial desmin .

用免疫细胞化学法观察了正常人肝贮脂细胞表达结蛋白中间丝。

Expressin of desmin filaments in fat storing cell ( FSC ) of normal human liver was observed by immunocytochemical method .

对照组心脏标本中可见瘢痕组织；心肌细胞结蛋白染色阴性，肌钙蛋白I染色阳性。

Scar tissue was seen in the control group , and desmin was negative and Troponin I was positive in cardiomyocytes .

部分诱导后的细胞胞浆结蛋白，心肌特异性肌钙蛋白，连接蛋白-43免疫组化呈阳性反应。

The immunohistochemical method results showed positive reaction of part of the cell plasm desmin , myocardium specific troponin and connexin-43 after induction .

主要观察指标：各组骨骼肌组织细胞微细结构和超微结构及结蛋白和波形蛋白的表达。

MAIN OUTCOME MEASURES : Microstructure and ultrastructure of skeletal muscles were observed , as well as the expression of desmin and vimentin .

目的探讨不同浓度的雄激素对骨骼肌卫星细胞中结蛋白基因及结蛋白表达的影响。

Objective To investigate the effect of androgen with different concentration on gene and protein expression of desmin in satellite cell of skeletal muscle .

结蛋白可作为肌细胞分化的标志，提示南宁与宁波青年冠状动脉内膜平滑肌细胞向成熟分化，而北京人则缺乏这种倾向。

It indicates that the SMC in the intima of the coronary artery are potentially differentiated into maturity in the youth of Nanning and Ningbo .

结论骨髓细胞梗死心肌移植对梗死区心肌组织结构起到保护作用，使部分心肌细胞结蛋白的分布恢复正常，但对梗死区心肌细胞结蛋白的表达量无影响。

Conclusion Transplantation of bone marrow cells to the acute infarct site can protect the myocardial structure and restore the normal distribution of myocardial desmin .

结果表明正常人肝贮脂细胞表达结蛋白，可作为研究该细胞的良好标志，并支持了贮脂细胞的肌源性学说。

The results show that FSC in normal human liver expresses desmin filaments and the immunocytochemical staining of desmin may be applied to identify FSC .