肝星状细胞

方法（1）采用完全无血清培养，用3HTDR掺入检测了PDGF、转化生长因子β1（TGFβ1）和表皮生长因子（EGF）对肝星状细胞的DNA合成作用；

Methods 3H TdR incorporation was used to estimate the DNA synthesis elicited by PDGF , TGF β 1 and EGF in HSC cultured in an absolutely serum free medium .

因此肝星状细胞已经成为肝纤维化研究中的重点和防治肝纤维化的重要靶点。

Therefore , HSC has been focus in the study about HF and become an important target for treatment of HF .

核因子κB与肝星状细胞增殖及凋亡

Nuclear Factor - κ B in Proliferation and Apoptosis in Rat Hepatic Stellate Cells

中药复方861抑制肝星状细胞NF-κB活性的体外研究

Herbal compound 861 inhibits NF - κ B binding activity of hepatic stellate cells in vitro

丹酚酸B盐对转化生长因子-β1刺激肝星状细胞活化与胞内信号转导的作用

Effects of salvianolic acid - B on TGF - β 1 stimulated hepatic stellate cell activation and its intracellular signaling

丹酚酸B对MDA刺激的肝星状细胞增殖的抑制作用

Inhibitory effect of salvianolic acid B on malondialdehyde-stimulated proliferation of rat hepatic stellate cells in vitro

血管紧张素II和洛沙坦对肝星状细胞生长、增殖的影响

Effects of angiotensin II and losartan on the growth and proliferation of hepatic stellate cells

重组副腺病毒介导的大鼠星状细胞激活相关蛋白基因转染对大鼠肝星状细胞表达间质胶原酶mRNA的影响

Effects of rAAV / rSTAP on mRNA level of interstitial collagenase in rat stellate cells

RT-PCR法检测肝星状细胞中Ⅰ型胶原mRNA的表达。

HSCs were harvested to measure the collagen ⅰ mRNA expression with RT-PCR .

复方861对人肝星状细胞α-平滑肌肌动蛋白mRNA表达的调节作用

Effects of Compound 861 on expression of a-smooth muscle actin mRNA in human hepatic stellate cells

方法分离、培养人肝星状细胞，用于TNFα刺激。

Methods Human hepatic stellate cells were isolated , cultured and stimulated by TNF α .

RNA干扰技术阻断黏着斑激酶表达对肝星状细胞增殖与凋亡的影响

Effects of Focal Adhesion Kinase Gene Silencing on Proliferation and Apoptosis of Hepatic Stellate Cells

维生素E和硒对肝星状细胞中金属蛋白酶1组织抑制剂mRNA表达的影响

Effect of Vitamin E and Selenium on the Expression of Tissue-inhibitor of Metalloproteinase-1 mRNA in Hepatic Stellate Cells

而TGFβ1在本实验条件下对肝星状细胞的增殖没有影响。

TGF β 1 gave no effects on the proliferation of HSC under current experimental condition .

小干扰RNA对肝星状细胞基质金属蛋白酶组织抑制因子-1表达的抑制作用

Suppression of Tissue Inhibitor of Metalloproteinase-1 on Gene Expression by Small Interfering RNA in Rat Hepatic Stellate Cell

IFNγ影响肝星状细胞α1（Ⅰ）mRNA表达信号的传递途径

IFN γ affects collagen α _1 (ⅰ) mRNA gene expression signals transduction pathway in rat hepatic stellate cells

TIMP-1特异性小干扰RNA真核表达载体转染肝星状细胞效率的检测

Detection of transfection efficiency of TIMP-1 targeted siRNA eukaryotic expression vectors in hepatic stellate cells

结论IL-10可通过PDGF/MAPK信号通路抑制肝星状细胞激活。

Conclusion IL-10 inhibits activation of HSC through the PDGF / MAPK pathway .

表皮生长因子对肝星状细胞c-fos基因表达的影响

Epidermal growth factor may influence the c-fos gene expression of hepatic stellate cell

罗格列酮对肝星状细胞MMP-2、TIMP-1mRNA表达的影响

Effects of Rosiglitazone on expressions of MMP-2 and TIMP-1 mRNA in hepatic stellate cells

靶向leptin基因的siRNA对大鼠肝星状细胞生物学特性的影响

Effect of siRNA Targeting Leptin Gene on Biological Behaviors of Rat Hepatic Stellate Cells

收获肝星状细胞，提取蛋白质，用固相pH梯度双向凝胶电泳方法进行蛋白质点的分离，获得分辨率和重复性均较好的双向电泳银染图谱。

Two dimensional polyacrylamide gel electrophoresis is used to separate protein spots , and the good 2-DE pattern including high resolution and reproducibility was obtained .

研究表明活化的肝星状细胞（hepaticstellatecellHSC）是肝纤维化过程中ECM的主要合成细胞；

Studies indicated that activated hepatic stellate cell ( HSC ) is the primary ECM-producing cells in hepatic fibrogenesis ;

方法：体外分离、培养大鼠肝星状细胞，甘草酸与HSC共同孵育。

Methods : The mice HSC were isolated and cultured in vitro .

蛋白酶活化受体2对大鼠肝星状细胞分泌MCP-1的诱导作用

Protease-activated receptor 2 challenges rat hepatic stellate cell to produce MCP-1

转染金属硫蛋白基因1A促进大鼠肝星状细胞增殖

Metallothionein gene transfected enhancing the proliferation of hepatic stellate cells

而肝脏内主要胶原合成细胞&肝星状细胞（Hepaticstellatecells，HSC）的激活、增殖则是肝纤维化发生的中心环节。

The activation and proliferation of hepatic stellate cells ( HSC )( major collagen-synthesizing cells ) are key links of liver fibrosis .

目的研究黄芪总提物（TEA）对体外肝星状细胞增殖和产生胶原的影响。

Aim To study the effects of total extract of astragalus ( TEA ) on activating the primary cultured hepatic stellate cells ( HSC ) and synthesizing collagen .

IL-1β促肝星状细胞增殖与JNK/AP-1信号转导通路的关系

Relationship between interleukin-1 β - induced proliferation of hepatic stellate cells and JNK / AP-1 signal transduction pathway

[目的]提高siRNA表达载体在肝星状细胞（Hepaticstellatecells，HSCs）中的转染效率。

[ Objective ] To increase the transfection efficiency of siRNA expression constructs transfection in hepatic stellate cells in vitro .