肿瘤基因

离体人乳腺上皮细胞致癌过程中c-myc肿瘤基因的变化

Alteration of c-myc oncogene in the human breast epithelial cell line treated by chemical carcinogens in vitro

目的：设计构建重组体为转染肿瘤细胞，观察癌基因的沉默效果，为探索肿瘤基因治疗的新途径打好基础。

Objective : To design and establish transfection tumor cells from recombinant , observe dumbness effect of oncogene , and explore the new method of gene therapy .

RNA干扰在肿瘤基因治疗中的应用策略

Strategies of RNA Interference in Gene Therapy of Tumor Disease

目的：探讨白细胞介素7裸DNA质粒用于小鼠肿瘤基因治疗的可行性。

Objective : To investigate the possibility of gene therapy with naked plasmid encoding interleukin 7 ( IL 7 ) .

通过抗敏低聚核苷酸阻断肿瘤基因c鄄myc的表达；

Blocking of the expression of the oncogene c-myc by antisense oligonuleotides ;

小干扰RNA及其在肿瘤基因治疗的应用

Application of SiRNA in Gene Therapy of Cancer

DNA微阵列技术以其高通量、自动化、平行化、快速化的优势，成为后基因组时代在转录组水平进行肿瘤基因表达谱分析的最强有力工具。

With the advantages of high throughput , automation and speed , DNA microarray is becoming the most powerful tool in cancer research in the post-genome age .

目的在肿瘤基因治疗研究中建立一个用绿色荧光蛋白（greenfluorescentprotein，GFP）为标记基因的合适条件。

Objective To search the appropriate experimental conditions for using green fluorescent protein ( GFP ) as a reporter gene in tumor gene therapy .

Wilms肿瘤基因反义寡苷酸对白血病细胞凋亡的影响

Effect of WT1 antisense oligonucleotide on apoptosis of leukemic cells

结论：HT病变组织中不同程度地存在与PTC相同的肿瘤基因，HT合并的PTC可能与HT有关。

Conclusions There exists the PTC oncogene products in the tissue of HT . PTC in HT may be associated with HT .

目的：构建并制备含人B7-1基因的重组腺病毒（Ad-B7-1）载体，为后续的肿瘤基因治疗研究提供实验基础。

Objective : To construct adenovirus vector carrying human B7 1 gene for tumor gene therapy .

目的克隆人胃癌细胞端粒酶RNA组分（hTR）基因片段并构建其正义和反义真核表达载体，为以端粒酶为靶目标的肿瘤基因治疗奠定基础。

AIM To clone human telomerase RNA component ( hTR ) gene from human gastric cancer cells and construct its eukaryotic sense and antisense expression vector .

目的：分离肺腺癌及同源正常组织中的未知特异性基因片段，并建立相应的cDNA文库，为研究肿瘤基因疫苗打下良好的基础。

Objective : To isolate unknown specific gene fragment of lung adenocarcinoma tissue and homologous normal tissue and construct their corresponding cDNA library and do the groundwork for tumor gene vaccine .

[目的]利用RNA干扰技术，以Bcl-2为靶基因，设计构建重组体，并进行序列分析，探索肿瘤基因治疗的新途径。

[ Purpose ] To study the new approach of tumor gene therapy by constructing recombinant using Bcl-2 as targeting gene with the help of RNA interfering and by conducting sequence analysis .

目的：构建人粒细胞巨噬细胞集落刺激因子（GMCSF）基因的重组腺病毒载体，为进一步研究GMCSF基因在肿瘤基因治疗中的应用提供实验基础。

OBJECTIVE : To construct recombinant adenovirus vector containing human granulocyte-macrophage colony-stimulating factor ( GM-CSF ) gene , and provide the basis of further research of GM-CSF gene for application .

IFN通过与靶细胞表面的受体结合后，激活信号通路，诱导相关抗病毒、抗肿瘤基因的转录和表达，发挥其生物学作用。

Binding of IFN to a cell surface receptor mediates activation of the signal transduction pathway that elicits the biological functions by inducing the transcription and expression of IFN-related genes .

ehCGβ肿瘤基因疫苗诱生的效应脾细胞过继免疫抗肿瘤作用的研究

Anti-Tumor Effects of Adoptively Transferred Splenocytes Induced by ehCG β Gene Immunization

目的构建正、反义hTERT基因真核表达载体，为进一步研究端粒酶的活性调节与恶性肿瘤基因治疗作准备。

Objective To construct eukaryotic expression vectors for sense and antisense human telomerase reverse transcriptase ( hTERT ), to facilitate further study of the regulation of telomerase activity and gene therapy of malignant tumors .

目的探讨ZNF217肿瘤基因（锌指蛋白217）在卵巢浆液性上皮癌20号染色体的表达情况，以及与卵巢癌的分级及预后的关系。

Objective To investigate the amplification of zinc finger protein 217 ( ZNF217 ) gene on chromosome 20 in ovarian cancer and its clinical significance .

由于ILK在细胞内和细胞外信号传递中起着关键的作用，而且抑制ILK的活性能够导致细胞周期的停滞和细胞凋亡的启动，使其成为肿瘤基因治疗和肿瘤药物的理想靶点。

Since the crucial role of ILK in transmiting intracellular and extracellular signals , as well as the cell cycle arrest and the initiation of cell apoptosis by suppressing the activity of ILK , ILK becomes an attractive target for tumor gene and medicine therapeutics .

利用动态时轴弯曲（DTW）作为分类器，在标准的肿瘤基因芯片数据的识别结果表明，本方法能够达到100%的识别率，可以应用于基因芯片数据的识别、分类和基因疾病推断。

With the classifier of Dynamic Time Warping , the recognition rates on standard tumor gene chip data show the good classification performance of 100 % of our method . Hence , it could be used for gene recognition , gene classification and disease inference based on gene chip data .

改进反向点杂交方法检测脑肿瘤基因突变

Detection of gene mutation in brain tumor with modified reverse dot blot

靶向性导入系统在肿瘤基因治疗中有极其重要的作用。

Selectively transfer system plays an important role of cancer gene therapy .

用于肿瘤基因治疗载体的肿瘤特异性配体研究进展

Tumor-targeting ligands used in gene therapy vector : research progress

抑癌基因p~（16）与肿瘤基因治疗

Tumor Suppressor Gene p ~ ( 16 ) and Gene therapy of Cancer

端粒酶逆转录酶基因在肿瘤基因治疗与诊断中的研究

The Effect of Telomerase Reverse Transcriptase in Gene Treatment and Diagnosis on Cancer

肿瘤基因治疗载体在临床应用中的问题和研究进展

The problem and progress of gene therapy vectors of tumor in clinical application

神经干细胞在恶性脑肿瘤基因治疗中的应用现状及前景

Application and prospect of neural stem cells in gene therapy of malignant brain tumor

胸苷激酶同功酶与肿瘤基因治疗

Thymidine Kinase Isoenzymes and The Gene-therapy of Cancer

选择合适的载体一直是肿瘤基因治疗研究中的重点。

Choose the right carrier has been the focus of the cancer gene therapy research .