胰酶

方法：将63例病人随机分成2组，胰酶胶囊组33例给予胰酶胶囊2粒，po，tid×4wk；

METHODS : Sixty three patients were divided randomly into two groups . Thirty three patients of pancreatin capsules group were treated with pancreatin capsules , ( two capsules ) po , tid for 4 wk .

本文对低温软化酶EB的性能及应用特点进行了研究，确定了其中胶原酶、非胶原酶的蛋白水解活力与温度、时间的关系并与工业胰酶等的软化性能进行了对比。

In this paper , we studied the properties and application of the low temperature bating enzyme EB and found out the relationship between hydrolytic activity of collagenase and non-collagenase with temperature and time and made a comparison about bating effect of EB and pancreatin in leather processing .

方法：用胰酶、胶原酶混合消化法获得阴道黏膜细胞。以丝裂酶素C处理的小鼠成纤维细胞作滋养层细胞。

Methods : Vaginal mucosa cells could be harvested from human vagina mucosa treated with trypsin-collagenase digesting .

毒素活性的最适pH为6&8，对胰酶有抗性；

The optimal pH for the toxin was 6-8 and resistant to trypsase .

此抗体对E玫瑰花生成有明显阻断作用，其相应抗原又与E受体一样对胰酶消化敏感。

The corresponding antigen was sensitive to trypsin digestion .

胰酶消化法纯化诱导不同年龄段SD大鼠骨髓破骨细胞的体外培养

Isolation of Bone Marrow-derived Rat Osteoclast-like Cells with the Digestion of Trypsin

考虑到传统的胰酶消化速度不好控制，我们用木瓜酶结合DNA酶I序贯消化。

We have modified the procedure to a sequential digestion of papain and DNase I for taking into account the fast traditional trypsin digestion .

采用冷胰酶消化法分离培养大鼠睾丸支持细胞，采用单细胞凝胶电泳法检测亚硝酸钠对大鼠睾丸支持细胞DNA的损伤作用。

DNA damage in somatic cells was determined by single cell gel electrophoresis ( SCGE ), and the relationship between DNA damage and HQ dosage was analyzed .

方法胰酶消化法分离、培养新生SD大鼠CFs，采用硝酸还原酶法和分光光度法分别测定基础状态和AVP干预下CFs的NOS活性和NO含量。

METHODS CFs were isolated by trypsin digestion method .

利用加入氨甲喋呤和胸苷使细胞分裂同步化并结合胰酶G带技术，分析了猪前中期染色体高分辨G带。

Analysis of high resolution G bands of prometaphase chromosomes in pigs was carried out by cell synchronization using amethopterin and thymidine treatment combining GTG technique .

通过试验，筛选出对水貂肠道冠状病毒敏感的貂肺传代细胞系（ML），经胰酶处理后，从6份病貂粪样中分离获得2株冠状病毒。

Two strains of coronavirus were isolated from 6 fecal samples of infected minks with mink lung cell line ( ML ) cultures .

90%融合时胰酶传代，于第12天收集第二代软骨细胞，制成浓度为5.0×10~7／ml细胞悬液；

The second passage was collected after 12 days and suspended at 5 . 0 x 107 / ml for the next experiment .

用胰酶和胶原酶消化法从1～2d新生大鼠的心肌组织中分离心肌细胞，用细胞计数法测出不同的消化酶分离所得的心肌细胞数量。

Neonatal rat cardiac myocyte was isolated using trypsin and collagenase solution . The number of isolated cell was counted by cell counter .

类胰蛋白酶和胰酶能诱导大量乳铁蛋白和IL-8的释放，而凝血酶和弹性蛋白酶却不能。

Tryptase and trypsin , but not thrombin and elastase induced significant lactoferrin and IL-8 secretion from neutrophils .

作者应用胰酶G带分染法对苏大白、杜洛克及正在培育的甘肃白猪和甘肃黑猪的染色体的G带核型进行了研究。

By means of trypsin-banding stains , the G-bandes karyotypes of the chromosomes of Large White , Duroc , Gansu White pigs and Gansu Black pigs have been studied .

作者注意到，大部分CF患者伴有严重胰腺外分泌功能不全，而且必须采取胰酶替代治疗。

Most CF patients have severe exocrine pancreatic insufficiency and must take pancreatic enzyme replacement therapy , the authors note .

方法：用胰酶消化法培养兔RPE细胞并传代。

Methods : The rabbit RPE cells were cultured with trypsin enzyme-digesting technique and passaged in vitro .

方法：1.采用胰酶消化法获得Wistar大鼠CFs。采用倒置相差显微镜和波形蛋白免疫荧光法鉴定细胞。

CFs were isolated by trypsin digestion method from Wistar rats and identified by invert microscope and vimentin immunofluorescence . 2 .

SST抑制胰酶分泌和胆汁分泌，还抑制内分泌激素如分泌素、胰岛素、胰高血糖素和血管活性肠肽的分泌。

SST inhibits pancreatic enzyme secretion and bile secretion . It also inhibits the secretion of secretin , insulin , glucagons and vasoactive intestinal peptide .

方法：取手术中切除的多余粘膜，分别用胰酶和Dispase分离后经胰酶消化，接种无血清培养基。

Methods : OMEC were primarily cultured with trypsin or dispase digestion .

胰酶对质膜H+-ATPase活性的活化谱型也存在差异，说明该酶C末端的结构或性质发生了变化。

The data obtained following trypsin treatment showed a difference in the enzyme activity pattern , suggesting that there existed a possible change in the C-terminus of the ATPase , either in the structure or in the property or both of them .

目的：探讨猪主动脉（PA）、胰酶处理PA（TPA）、戊二醛处理PA（GPA）的结构及免疫原性。

Objective : To study the structure of porcine aortas treated with glutaraldehyde ( GPA ), trypsin ( TPA ) or nothing ( PA ) and their immunogenicity .

应用先PBS洗涤后0.25%胰酶消化的传代方法易于控制，消化效果好。

It is easy to control and have good digest effect that use PBS wash the cells advance then apply 0.25 % trypsin to digest .

M6的胶体金复合物不能与经胰酶和神经氨酸酶处理过的红细胞发生结合。

M6 gold complexes showed no ability to bind to surface of erythrocytes treated by trypsin or neuraminidase .

方法胰酶消化法分离和培养KM乳鼠心肌细胞，按照1×105ml的密度接种于96孔培养板。

Methods We isolated and cultured cardiomyocytes in neonatal mice by trypsin digestion technique . The cells were inoculated in 96-well culture plate at the density of 1 × 10 5 / ml.

用Massey法从胰酶处理牛乳中提纯黄嘌呤氧化酶，但对其程续进行某些改进。

Massey 's method was modified for the purification of xanthine oxidase from pancreatin-treated milk .

方法：骨髓髓腔冲洗获得新西兰大白兔幼兔股骨骨髓，用全骨髓培养法进行体外培养获得BMSCs，胰酶消化传代，用条件培养基培养传代细胞。

Method : Bone marrow was harvested from rabbit femur bone marrow with flushing method .. Adherent cells were selected as BMSCs after the whole marrow was cultured .

方法用0.1%胰酶+0.02%乙二胺四乙酸（EDTA）消化人大网膜组织，经去除红细胞后培养。

Method Human greater omenta was enzymatic disaggregated with 0.1 % trypsin & 0.02 % ethylene diaminetetraacetic acid ( EDTA ), and cultured after isolation from red blood cells .

取每只大鼠左眼作视网膜胰酶消化铺片，并在铺片上进行血管细胞粘附分子-1（VCAM-1）单克隆抗体免疫荧光组织化学研究。

Retinal digest preparations were made and immunofluorescence of the vascular cell adhesion molecule 1 ( VCAM 1 ) was detected with monoclonal antibody .

用3.5%聚乙二醇（PEG）沉淀血清中的1G，再以胰酶消化，在抗体过量时CA的得率可达100%。

1C in the serum precipitated by 3.5 % polyethylene glyco ( PEG ) was digested by trypsin . The recovery rate of CA in antibody excess serum were 100 % .