超低温

方法将切除下来的脑胶质瘤制成肿瘤组织匀浆，超低温保存，备用。

Methods Homogenate made from resected brain gliomas was conserved under ultralow temperature for use .

以液氮超低温保藏最理想，酵母细胞存活率较高；

The yeast cells might as well be stored ultralow temperature by liquid nitrogen for higher survival rate ;

拟南芥幼苗超低温保存后DNA甲基化的遗传变异

Variation of DNA Methylation in Arabidopsis thaliana Seedlings After the Cryopreservation

计算机辅助解析BR蛋白在超低温下二级结构变化

Computer-aided identification the secondary structure of BR at low temperature

超低温高电场下GaAs的电子太赫兹功耗谱的研究

Terahertz power dissipation spectra of electrons in bulk GaAs under high electric fields at low temperature

M2钢铣刀的超低温处理

Cryogenic Treatment of M2 Steel Milling Cutter

最后剔除断裂及降解的、纯度较低的DNA样本，超低温保存，从而建立了中国少数民族基因组DNA样本库。

At last , we removed the DNA samples of breakage and degradation or lower purity , thereby establishing a genomic DNA library of Chinese ethnic minorities .

超低温冻存前后胎儿脐血中NK细胞活性的研究

Experimental Study on Natural Killer Cells Activity of Fetal Cord Blood Pre - and Post - frozen

本文介绍LNG超低温阀门的计算分析过程，针对液化天然气的特点设计超低温阀门的结构。

The article designs the structure of LNG cryogenic valves according the characteristics of liquefied natural gas .

超低温处理对M2钢铣刀耐磨性的影响

The Influence of Super Low Temperature Treatment on the abrasion resistance of Milling Cutters of M2 Steel

2采用超低温处理去除Wistar大鼠颈总动脉（内径约1mm）的内皮细胞。

Wistar rat artery was treated by super low temperature .

超低温亚干式冷却切削系统（MOL技术）

Cutting system with super low - temperature sub - dry typed cooling ( MOL technique )

BAC克隆以单克隆形式接种于384孔板中，保存在-80℃的超低温冰箱中。

BAC clone was stored in 384-well microtiter plates .

过去的几十年里，超低温探头技术是使得NMR灵敏度得到极大提高的技术上的唯一进步。

Bruker 's CryoProbes ( superscript TM ) show large improvement in terms of NMR detection sensitivity over the last few decades .

方法取Wistar大鼠坐骨神经，经超低温冷冻保存后移植于SD大鼠坐骨神经缺损处。

Methods The sciatic nerve defect at SD rats was bridged by the sciatic nerve from wistar rats after ultra deep cryopreservation .

芸芥（ErucasativaMill）发状根的诱导、超低温保存及次生代谢物的分析

Induction and Cryopreservation of Eruca Sativa Mill Hairy Roots and Analysis of Its Secondary Metabolites

分成超低温冷冻同种神经移植组（A）、新鲜同种神经移植组（B）、及自体神经移植组（C）。

They were divided into 3 groups : ultra deep cryopreservation homologous nerve in group ( A ), fresh homologous nerve in group ( B ), and autologous nerve in group ( C ) .

从1985年1月至1986年2月我库用超低温冻贮精液实施供精人工授精（AID）118例，确知怀孕及生育者33例。

From Jan of 1985 to Feb of 1986 , 118 wifes , totally 181 menstrual cycles were inseminated with cryopreserved donor semen , and 33 pregnancies were proved .

本文主要探讨了M2钢铣刀经超低温处理后耐磨性的变化情况。

This paper mainly deals with the abrasion resistance variation of milling cutters of M2 steel by using super low temperature treatment .

本文通过在超低温下的V型缺口夏比冲击试验和SEM断口分析研究了高锰奥氏体钢的冲击韧性及其影响因素。

The extra-cryogenic impact toughness and influence factors of the high-Mn austenitic steel are studied by means of the Charpy V-notch impact test and the SEM fractured appearance analysis .

PTCR陶瓷材料的超低温烧结

Super Low Temperature Sintering for PTCR Ceramics

RT-PCR及Western所用标本为手术中切除新鲜组织，切除后立即存放在液氮中，然后冻存于-80℃超低温冰柜中手术切除标本均经组织病理学确诊。

Samples for RT-PCT and Western blotting were fixed immediately in liquid nitrogen after surgery , stored at - 80 ℃ in ultra-low temperature freezer . All samples were confirmed by histopathology .

方法取Wistar大鼠股动脉，经超低温冷冻保存后行血管细胞培养及动脉移植；

Methods Femoral artery was harvested from Wistar rats . The cell culture of the vessel and artery transplantation were carried out after cryopreservation .

光棘豆（OxytropisleptophyllaDC）愈伤组织超低温冰冻保存技术的研究

The cryopreservation of Oxytropis leptophylla callus

混合轴承陶瓷球在超低温重载条件下工作6h发生表面局部剥落。

Surface spalling took place on hybrid bearing balls under heavy loads and extremely low temperature conditions in six hours .

主要研究了M2钢铣刀超低温处理局的组织性能变化。

The changes of the microstructure and the properties of M2 steel milling cutter after being treated under low temperatures are studied in this paper .

Al-Zn-Mg合金超低温高速大变形中超细晶组织演化行为的研究

Microstructural Evolution of Ultrafine-Grained Al-Zn-Mg Alloy during High Speed and Large Plastic Deformation at Cryogenic Temperature

超低温保存前后太平洋牡蛎精子（Crassostreagigas（Thunberg））超微结构观察

The Morphological and Ultrastructural Variation of Pacific Oyster ( Crassostrea gigas ( Thunberg )) Sperm After Cryopreservation

采用不同的拟南芥ABA突变体，超低温保存后研究其存活率的不同，进而探讨ABA信号转导途径与低温胁迫，脱水胁迫信号转导途径间的相互作用。

Cells of different ABA synthesis or response mutants of Arabidopsis thaliana had different viability after cryopreservation , which revealed ABA has a closed relationship with cold - and dehydration-stress signaling transduction .

采用玻璃化法成功地超低温保存了胡萝卜悬浮培养细胞及其原生质体，存活率分别为83.3%（TTC法检测）和47%（FDA法检测）。

The survival rates for carrot cells and protoplasts cryopreserved by vitrification were 83.3 % ( based on the TTC reduction ) and 47 % ( based on the FDA assay ) respectively .