转录起始

IL-8在AP中存在高度表达，IL-8在转录起始点存在基因多态性，通过转录调控影响IL-8的合成。

It has high expression in AP which has gene polymorphism in transcription initiation , by which the synthesis of IL-8 can be regulated .

鸡卵清蛋白基因转录起始点的确定及表达载体的构建

Determination of Transcription Initiation Site of Chicken Ovalbumin Gene and Construction of Its Expression Vector

启动子是DNA序列上的一段重要的基因调控序列，标志着转录起始点的位置，可以用来定位基因。

Promoter is a gene regulatory DNA sequence , which indicates the location of Transport Start Site and can locate gene .

启动子是指导基因转录起始重要作用的序列段，在DNA序列中可能处于多个位置。

It is an important sequence segment for promoter to instruct the transfer of gene . Promoters can be on several locations in DNA sequence .

5′RACE分析确定其转录起始位点是269nt处的A。

Transcriptional initiation site A , which is at the 269 nucleotide , was preliminarily determined by using 5 ′ RACE method .

在转录起始点上游137bp定点将A突变为C后，对化学启动子的化学诱导应答性没有明显的影响，但对诱导效应的向上运输存在一定程度的阻抑作用。

Specific mutation from A to C at - 137 bp site upstream from transcription start point had no effect on the inducibility of the promoter in response to SA and BTH .

采用瞬时转染的方法，通过荧光素酶报告基因实验检测eNOS基因转录起始点上游长16kb启动子区域的活性。

Promoter activity of eNOS gene was determined by luciferase reporter gene assay .

启动子是一段供RNA聚合酶定位用的，位于基因转录起始点上游并对基因转录起调控作用的DNA序列，也是基因表达调控的重要顺式元件之一。

Promoter is a DNA sequence used for RNA polymerase combinating , and is an important cis-acting element in the regulation of the gene expression . It locate at upstream of initiation site of transcription .

分离植物目的基因全长cDNA和启动子的新方法&快速定位转录起始位点（RITIS）（英文）

Isolation of Full-length cDNA and Promoter of Target Gene from Plant by Rapid Identification of Transcriptional Initiation Site ( RITIS )

通过RT-PCR的方法确定OsGCS基因的转录起始位点可能位于翻译起始位点（ATG）上游211bp处。

The putative transcription start site ( TSS ) confirmed by RT-PCR was located 211 bp upstream of the translation start codon ' ATG ' ( Fig.5 ) .

该方法通过对转录起始位点（TSS）信息，调控区、编码区组成成分特征信息及CpG岛相关信息的综合来预测人类基因组启动子。

The method makes predictions of promoter by combining information about transcription start sites ( TSS ), compositional features in coding and regulatory regions and CpG islands .

真核细胞转录起始因子4E表达与乳腺癌血管生成及淋巴结转移的关系

Relationship among expression of eukaryotic initiation factor 4E , tumor angiogenesis and lymph node metastasis in breast cancer

通过PCR方法对克隆的两个启动子进行定点突变，使转录起始位点上游-137bp处A突变为C，得到两个突变启动子（IPMl、IPMs）。

By artificially changing A to C at - 137 bp site upstream from transcription start point of cloned promoter , two site-mutation promoters , IPM s ( 603 bp ) and IPM 1 ( 900 bp ) were created .

尽管目前在绵羊的FSHR基因只发现1个转录起始点，但启动子区的序列特征表明牛羊可能存在第2转录起始点。

Although only one transcription start point found at sheep currently , another start point might exist on FSHR genes of sheep , goat and cattle ;

采用5′RACE方法确定鸡卵清蛋白基因转录起始点的位置，通过序列分析得出转录起始点为G，从而确定出核心启动子及上游调控区的位置。

To determine the core promoter of chicken ovalbumin gene and 5 ′ upstream regions , the transcription initiation site of ovalbumin gene was confirmed by 5 ′ RACE method , at the same time , the regulatory elements of chicken ovalbumin gene were determined by sequence analysis .

采用引物延伸法，对ApNPV核多角体蛋白mRNA转录起始点进行了测定，确定其位于该基因调控序列12个核苷酸高保守区的nt&50位点与AcNPV相似。

The mRNA transcriptional start site was determined by primer extension , which located at nt - 50 in the high conserved 12-mer nucleotide domain , similar as AcNPV .

本文应用改良的引物延伸法，鉴定了一个小麦的苯丙氨酸解氨酶和几丁质酶基因的转录起始点。这种方法只需少量同位素，SephadexG－25柱和严格的杂交条件。

An improved procedure for primer extension analysis was used to identify the transcription start site of a phenylalanine ammonia-lyase ( PAL ) gene and a chitinase gene from wheat . This procedure requires less amount of isotope , a Sephadex G-25 column and highly stringent hybridization conditions .

IRX1基因转录起始点上游存在高甲基化结构基础。

There is a hypermethylation sequential basis in the upstream region from the transcriptional start site of IRX1 gene .

首先利用RT-PCR和引物延伸方法确定了hb1f（nr5a2）基因的转录起始位点，并通过序列缺失方法鉴定出hb1f（nr5a2）基因的核心启动子。

We determined the transcription start site ( TSS ) of hb1f ( nr5a2 ) gene via RT-PCR and primer extension and then identified the promoter of hb1f ( nr5a2 ) gene , which exhibited high hepatocyte specific activity .

与其它测定基因转录起始位点的方法相比，RLM-RACE方法更快捷、简便、准确。

RLM-RACE ( RNA ligase-mediated rapid amplification of 5 ′ and 3 ′ cDNA ends ) is more rapid and simple comparing with the common methods for determining gene transcription initiation sites .

并发现在BFV3026的内部启动子近上游存在神秘转录起始点，该起始点在泡沫病毒的自然感染进程中发挥作用。

And a cryptic start site of transcription was found , which was located upstream of the internal promoter TATA box and used in the natural infection .

结果（1）在正常对照、患者一级亲属和WD患者的启动子区-190、-78和+260位（转录起始点为+1）均发现存在单个碱基的不同；

Results ( 1 ) There were three polymorphisms at positions - 190 , - 78 , + 260 ( transcription start site as + 1 ) of the promoter region of WD gene . Normal control , WD patients and patients ' first-degree relatives all present the polymorphisms ;

但对核小体结合模体的相对偏好性进行比较后发现，转录起始位点、转录终止位点、起始密码子和终止密码子附近±500bp的序列所包含的偏好的核小体结合模体模式数各不相同。

Comparison of relative preference of nucleosome binding motifs shows that the mode number of preferred nucleosome motifs in ± 500bp region around the transcription start sites , transcription termination sites , start codon and stop codon is different .

实验结果表明，与正常脱硫基因启动子活性相比，脱硫基因转录起始位点上游300bp启动子序列具100℅活性，150bp为42%，小于75bp活性为0。

The results showed that the desulfurization gene transcription start site upstream 300 bp promoter sequences with 100 % activity , 150 bp to 42 % , less than 75 bp for 0 , compared with the normal dsz promoter activity in strain R-8 .

植物基因调控序列及其转录起始频率分析

Analysis of Regulatory Sequences and Transcriptional Initial Frequency of Plant Genes

植物基因转录起始频率分析

In silico analysis of transcriptional initiation frequency of plant genes

基于滑动窗口的原核转录起始位点计算定位方法

Computational location of transcription start sites in prokaryotic genome based on sliding window

真核生物Ⅱ类基因的转录起始

Transcription Initiation of Eukaryotic Class ⅱ Genes

小麦苯丙氨酸解氨酶基因和几丁质酶基因转录起始点的鉴定

Identification of the Transcription Start Sites of a Phenylalanine Ammonia-lyase Gene and a Chitinase Gene From Wheat

一组非组蛋白类的多肽对人胚肝细胞核体外转录起始的抑制作用

The Inhibition of Transcription Initiation of Nuclei From Human Embryonic Livers by a Group of Nonhistone Peptides in Vitro