透析法

方法：采用平衡透析法结合HPLC，对阿比朵尔的人血浆蛋白结合率进行测定。

METHODS : The equilibrium dialysis combined with HPLC to determine the plasma concentration and plasma protein binding rate of arbidol was carried out .

本文采用HPLC法测定血浆总色氨酸（T-Trp）及经平衡透析法分离的游离色氨酸（F&Trp）。

In this paper , the determination of T-Trp and F-Trp after equilibrium dialysis in serum by HPLC was reported .

采用溶剂注入法制备维A酸维生素C棕榈酸酯泡囊，并进行了处方优化，采用透析法测定包封率。

Prepare tretinoin ascorbyl palmitate vesicles by solvent-injection method , and optimize the formulation of vesicles , entrapment efficiency was determinated by dialysis method .

用透析法使麦芽假单胞菌HCG受体重组于脂质体的研究

Reconstitution of Pseudomonas maltophilia hCG receptor to liposomes by dialysis method

方法：采用平衡透析法，以HPLC法测定克班宁大鼠血浆中的蛋白结合率。

Methods Using the equilibrium dialysis and HPLC methods detection the binding rate of to plasma protein of rats .

透析法和稀释法复性重组人Cu，Zn-SOD包含体

Renaturation of Recombinant Human Cu , Zn-Superoxide Dismutase by Dilution and Dialysis

按平衡透析法测得本品血浆蛋白结合率为51.3±2.2%（（？）±SD，下同）。

The drug-plasma protein binding rate was found to be 51.3 ± 2.2 % (± SD ) using equilibrium dialysis method .

采用平衡透析法测定了灯盏乙素的血浆蛋白结合率，在血药浓度3.3-37.4μg／ml范围内灯盏乙素血浆蛋白结合率在90.59～93.87%之间，属于高度血浆蛋白结合的药物。

The ratio of scutellarin binding to human plasma was determined by equilibrium dialysis . When the plasma concentration of scutellarin was 3.3-37.4 μ g / ml , the biding ratio was 90.59-93.87 % .

通过逐渐降低缓冲液中尿素浓度的透析法对rMTG进行体外复性，比酶活达13.63U／mg蛋白，蛋白回收率为48.9%。

The specific activity and total protein recovery of MTG were 13.63U/mg and 48.9 % , respectively .

本法改变了透析法脱盐和离子交换柱层析等传统的分离纯化细胞色素C的繁琐过程，采用了分子筛色谱法脱盐和纯化.它具有简单、快速及纯度高的特点。

This method uses molecular sieve chromatography to desalt and cleans , transforms the complicated procedure of using dialysis in desalting and ion & exchange chromatography in cleansing cytochrome C and has the advantages of simplicity , quickness and high level purity .

用脑内透析法测定人参茎叶皂甙及其单体对大鼠纹状体DOPAC、HVA及5-HIAA的影响

Effect of GSLS and its monomers on DOPAC , HVA and 5-HIAA in rat striatum in vivo

方法：利用透析法和流释法检测37℃时活性炭微粒对5FU的吸附和缓释作用。

Methods : The absorptive and release effect was detected by dialysis and flow dialysis in 37 ℃ .

建立HPLC-UV法测定其含量及包封率，用动态透析法研究其释放度。氟脲嘧啶白蛋白微球剂的含量测定

HPLC method was established for determination of content of doxorubicin in albumin microspheres . Study on determination of 5 ─ fluorouracil in albumin microspheres

使用分步透析法对变性的包涵体进行复性，将复性蛋白过GST亲和层析柱得到纯化的GST-EO融合蛋白。

After the protein refolding of denaturant inclusion body following dialysis , we got the pure recombinant GST-EO protein by GST affinity columns .

B-Pluronic-PLA纳米粒子由透析法配制得到。

B-Pluronic-PLA nanoparticles were prepared by the dialysis method .

建立了微球的体外释药方法，确定了以pH7.4磷酸盐缓冲溶液为释放介质的透析法进行制剂体外释放的测定。

Dialysis method was selected to examine the drug release in vitro in pH7.4 phosphate buffer solution ( PBS ) as the medium .

用逐步稀释透析法将纯化的大肠杆菌表达的重组绿脓杆菌外毒素A（PEA）受体结合区蛋白初步复性，复性后的可溶性蛋白用于竞争抑制PEA的细胞毒性实验。

The purified recombinant receptor binding protein of exotoxin A of P aeruginosa expressed in E coli was renatured preliminarily by diluted progressively dialysis . The renatured soluble protein was used in the experiment of competitive inhibition for cytotoxicity of PEA .

方法：用分光光度法测定CYP450含量，用平衡透析法测定蛋白结合率。

Methods : the binding rate of DCPB to plasma protein was determined by equilibrium dialysis method , and the content of CYP450 was assayed by differential spectrum method .

用Ni-NTA柱纯化包涵体溶解物，目的蛋白洗脱液经多步透析法复性后冷冻抽干，获得一定量各种纯化蛋白。

Lysates of inclusion bodies were purified by using Ni-NTA column , and the target protein eluent was subjected to renaturation by step-wise dialysis and lyophilization to obtain the purified proteins . 6 .

方法：选择主要经肾脏排泄的CEZ为模型药，采用高效毛细管电泳法测定其血药浓度，并计算药代动力学参数；采用平衡透析法测定其血浆蛋白结合率。

Methods : The serum concentration of cefazolin and plasma protein binding rate in neijiang pig was detected by high performance capillary electrophoresis .

采用透析法研究aFGF阳离子脂质体体外释药动力学，考察其与aFGF溶液组的差别及释放介质对其释药的影响。

The vitro release kinetics of aFGF cationic liposome was investigated by Dialysis method . Observed the influence of drug release between different release medium , and also researched the difference with aFGF solution .

用透析法提取特异性转移因子（STF），经检验：STF蛋白反应呈阴性，OD260/OD280为2.38，原液中含有17种氨基酸。

Specific transfer factor ( STF ) was extracted by dialysis , and the result of detection : The protein was negative reaction , and OD260 / OD280 was 2.38 . The amino acid were 17 species in STF .

方法考察处方的粒径分布、Zeta电位、溶血性、沉降稳定性，用反透析法测其包封率、脂质体的血浆稳定性，观察其基本形态。

METHODS The particles size , Zeta potential , haemolyticus , and sedimentation stability were evaluated . Entrapment efficiency and the stability of liposomes in human plasma were determined by reverse dialysis method , and the shape of these nanoliposomes was observed by transmission electron microscopy .

采用胃蛋白酶消化法、动态透析法分别测定ADR-HSA-MS的载药量，体外释药性质；

Pepsin digestion method was used for determining drug loading of ADR-HSA-MS and membrane diffusion technique for detection of in vitro release of ADR from the microspheres .

本文应用平衡透析法在pH7.4，振动频率为140～160次/min，25℃恒温条件下，研究了喜树碱和秋水仙碱两种天然抗癌有效成分与人血清蛋白的相互作用。

The interactions between human serum album in ( HSA ) and camptothecine and colchicine were studied at pH 7 . 4,25 ℃ and frequency of vibration 140 to 160 Hz by using equilibrium dialysis method .

方法以透析法考察阿霉素明胶微球的体外释放度，采用紫外分光光度法在波长254nm处测定阿霉素明胶微球的体外释药量。

Methods Dialysis method was used to test the property of microspheres release in vitro , and the ultraviolet spectrophotometry was used to examine the release of adriamycin gelatin microspheres at 254 nm .

用动态透析法研究微球的体外释放特性。方法：采用动态透析技术测定ACM-SLN冻干针剂的体外释药百分率，用不同的方程对其释药百分率进行拟合。

Dynamic dialysis method was used to determine the releasing characteristic of microspheres in vitro . METHODS : The release was studied by dynamic dialysis method . Different equations were selected to fit the release law .

室温透析法测定微量血清的游离T4

The microdetermination of serum free t _4by dialysis at room temperature

动态透析法考察体外释药性质；

To measure the drug release in vitro by dynamic dialysis ;

方法乳化化学交联法制备微球，动态透析法进行体外释药，离体定位装置进行体外磁定位。

METHODS The microspheres were prepared by emulsion cross-linking techniques .