酸性磷酸酶染色

Hp（Toxin＋）引起的细胞空泡样变，吖啶橙及酸性磷酸酶染色阳性；

It was positive in vacuoles with acridine orange and acid phosphatase stain .

Berry硝酸铅法进行酸性磷酸酶染色，观察不同浓度，不同时间处理后的细胞溶酶体活性；

Acid phosphatase was examined by Berry method which responses the activity of lysosome ;

酸性磷酸酶染色显示包绕并吞噬分解外源蛋白的结构是呈染色阳性的溶酶体结构。

An acid phosphatase staining was performed in order to confirm that the autophagosome-like structures have lysosomal enzymes , the structures displayed positive staining .

22周后处死动物，取假体周围软组织用HE染色组织切片观察；取胫骨近端骨组织，制作切片，计数每个区域的抗酒石酸酸性磷酸酶染色阳性细胞的个数，取平均值。

The rabbits were sacrificed and obtained soft tissue around the prosthesis stained with HE histological observation . Sections of proximal tibia were counted each region tartrate-resistant acid phosphatase positive cells .

应用抗酒石酸酸性磷酸酶（TRAP）染色观察体外培养破骨细胞的生存时间

Observation of the survival period of osteoclasts cultivated in vitro with tartrate resistant acid phosphatase ( trap ) staining

结果皮质骨内层可培养出多核、体积大、抗酒石酸酸性磷酸酶（TRAP）染色阳性的破骨细胞，淫羊藿提取物对皮质骨来源的破骨细胞没有明显影响。

Results Large multinucleated TRAP-positive osteoclasts from cortical bone were cultured . No obvious effect of icaritin on mature osteoclast was observed .

抗酒石酸酸性磷酸酶（TRAP）染色观察OC形态变化，图像分析体外骨吸收陷窝面积，原子分光光度计法测定OC培养液上清液中Ca2+含量。

The OCs were observed by the TRAP stain . The area of bone resorption and the concentration of Ca 2 + in the supernatant liquid of OCs cultures were also tested .

经酸性磷酸酶（AcPase）染色证实自噬泡均呈AcPase阳性，第7天后轴突体被降解吸收，形成的空腔内偶见巨噬细胞。

The autophagic vacuoles exhibited acid phosphatase ( AcPase ) activities . Since the day 7 , the axon bodies were absorbed after degradation and macrophages could be spotted occasionally .