链终止

运用双脱氧链终止法对第7外显子阳性突变标本进行DNA测序。

In addition , Sanger method ( dideoxy mediated chain termination method ) of DNA sequencing was used to examine the exon 7 which was positive in p53 mutation .

用RTPCR法从人外周血单核细胞克隆了编码共刺激分子B71/CD80胞外区的cDNA，并用Sanger链终止法进行测序。

The cDNA encoding the extracellular domain of human costimulatory molecule B7 1 / CD80 was cloned from human peripheral blood monocytes by using RT PCR method and sequenced by Sanger chain termination method .

利用RT-nestedPCR方法检测感染率和基因分型，采用双脱氧链终止法测定HCV-PCR产物序列。

Infection rate and genotype were detected with RT nested PCR , HCV PCR sequence was detected with double deoxidation chain terminal method .

从聚合物分子量评价自由基聚合的链终止反应

Evaluation of chain termination in radical polymerization based on the molecular weights of polymers

链终止反应速度与活性中心浓度的二次方成正比。

The chain termination is second order with respect to the concentration of active species .

活性聚合活性聚合是指增长中心不会发生链终止和链转移的聚合反应。

Living polymerization are polymerizations in which propagation centers do not undergo either termination of tranfer .

同时，煤中固有的芳香大分子自由基也可与聚合物大分子自由基结合发生自由基链终止反应。

At the same time , the aroma macromolecule free radical of coal made the chain reaction end by combining with polymer macromolecule free radical .

卤代二乙基铝的影响效果与烯丙基卤不同，在本体系中起链终止作用。

The effect of halogen aluminium ethyl on the polybutadiene synthesis is not the same as that of allyl halide and the former acts as a chain end in the system .

扩增产物经纯化后通过一个以链终止法为基础的循环测序程序直接进行序列测定，其中所用四种双脱氧核苷酸分别带不同的荧光标记，测序引物选自同一组通用引物。

Products of amplification were directly sequenced by chain termination-based cycle sequencing protocol after being purified , in which four ddNTPs were separately labeled with four different fluoresceins , and primers used for PCR amplification were reemployed as sequencing primers .

采用双脱氧DNA链合成终止法进行PCR产物直接测序，确认基因型的测定结果。

The sequencing of the PCR product was determined by using dideoxy - mediated chain - termination method .

PCR产物纯化后直接经双脱氧链末端终止法测定核苷酸序列。

The amplified product with 238 bp was purified and then directly sequenced by dideoxy nucleotide chain termination method .

阳性重组质粒经PCR、酶切鉴定，用双脱氧链末端终止法进行序列测定。

The positive recombinant plasmid was identified by PCR , enzyme digestion . The nucleotide sequence of CSP3 ′ ending gene was determined by the dideoxy chain termination method .

IBVE/Cl2/TiCl4/CH2Cl2体系阳离子聚合链转移终止机理的研究

The Mechanism of Chain Termination in the Cationic Polymerization of IBVE Initiated by Cl_2 / TiCl_4 / CH_2Cl_2 System

方法应用逆转录巢式PCR技术，从38例III/2a型HCV感染患者血清中，扩增HCV部分包膜区基因片断（nt1460～1582，HCVJ6），纯化后直接采用双脱氧链末端终止法进行序列分析。

Methods The RNA ( nt1460 1582 , HCV J6 ) extracted from plasma of 38 patients infected with genotype III / 2a HCV were transcribed , amplified , purified and directly sequenced by using RT nested polymerase chain reaction ( PCR ) and dideoxynucleotide chain termination method , respectively .

自由基链的终止产生于双分子反应。

Termination of free radical chairs occurs by a bimolecular reaction .

热裂解链引发-终止反应的计算CH键的断裂-形成

Calculation for Chain Initiation-Termination Reactions in Thermal Cracking : Cleavage-Formation of C & H Bond

结果表明，链引发和链终止反应与嵌段链活性中心的形态有关，只有嵌段链活性中心的低活性形态才能同αMS发生引发反应，而高活性形态则与αMS发生链终止反应。

It was found that the chain initiation and termination reactions were dependent on the species of the block chain active centres and only the lower active species . could initiate α es while the higher active species were terminated rapidly by α MS.

根据实验结果，推测终止反应主要为接枝链间的偶合终止。反应后期的偶合终止对这个肩膀峰也有一定贡献。

Also coupled termination events contributed some to the shoulder peak .

这一过程为一可逆过程，从而控制了自由基的浓度，防止了链转移和链终止的发生。

This is the reversible process that controls the concentration of free radicals . It prevents the reaction of chain transfer and chain stop .

因此，煤基聚合物共混体系中，煤的含量较高时，由于煤芳香大分子的链转移和链终止占优势，煤的加入有助于提高聚合物的热稳定性和热氧稳定性。

The adding of coal in the blends are benefit to the thermal and thermal oxidation stability for the predominate of chain transfer and chain end in blends when high coal content .

本文考察了Fe（naph）2-Al（iBu）3卤化物体系催化丁二烯聚合的链引发、链增长和链终止反应。

Initiation , propagation and termination of the polymerization of butadiene catalyzed by Fe ( naph ) _2 Al ( I Bu ) _3 Halides system have been investigated .

离子液体作为自由基聚合的溶剂时，可以提高链增长速率常数，降低链终止速率常数，并获得更高的产率和产物分子量。

As radical polymerization solvent , ionic liquids could increase the propagation rate constant kp and reduce the chain termination rate constant kt , and higher yield and higher molecular weight were obtained as well .

通过链转移反应，在大分子自由基链终止的同时，形成了单体自由基，后者仍可与粘胶体中的单体发生链的引发、增长及转移等过程。

Therefore , the chain transfer between the growing chain radical and monomer will be the only process of termination .

计算了在不同聚合条件和各种催化剂组成时的链增长、对烷基铝链转移及链终止反应的速度常数。

The propagation , transfer and termination reaction rate constants have been calculated for different polymerization conditions and for catalysts of various compositions .

方法病毒粒RNA经逆转录合成cDNA，经聚合酶链反应（PCR）扩增，产物纯化，采用双脱氧链末端终止法进行核苷酸序列测定。

Methods Virion RNA was transcribed into cDNA by reverse transcriptase , cDNA amplified by PCR , the productions of PCR were purified .

方法：慢性乙型肝病患者血清中提取HBVdna，经巢式聚合酶链法（nested-PCR）扩增HBVs基因区片断，利用双脱氧链末端终止法检测HBVs区核苷酸序列并进行基因型分析。

Methods HBV DNA extracted from the 39 serum samples of chronic liver disease patients were amplified by nested polymerase chain reaction ( nested-PCR ) to obtain the S gene sequences , and all the PCR products were sequenced by dideoxy-chain-termination method .