限制性核酸内切酶
- 网络restriction endonuclease;restriction enzyme;endonuclease
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限制性核酸内切酶与DNA相互作用研究进展
Progress on the Interaction of Restriction Endonuclease and DNA
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Ⅱ型限制性核酸内切酶NcrⅠ的研究
Study on A Type ⅱ Restriction Endonuclease Ncr ⅰ
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人乳头瘤病毒DNA的限制性核酸内切酶分析
Restriction enzyme analysis of human papilloma virus ( hpv ) dna from common warts
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该法提取的血吸虫基因组DNA较纯,适合于以后进行DNA限制性核酸内切酶谱分析和Southernblot分析。
The technique is useful in the analysis of restriction endonuclease digestion of genomic DNA and Southern blot .
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本文应用限制性核酸内切酶HaeⅢ显示了孟氏裂头绦虫染色体G分带,获得了较好的G带带纹。
G-banding of the chromosomes meets with success by using restriction endo-nucleas Hae ⅲ in Spirometra mansoni .
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HSV临床分离株的限制性核酸内切酶酶切分析与鉴定
Restriction endonuclease cleavage analysis of DNAs from herpes simplex verus clinical isolates
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方法:用甲基化敏感的限制性核酸内切酶消化,结合聚合酶链反应(PCR)技术。
Methods : 20 cases of MDS patients were studied using methylation sensitive restriction enzyme digestion and polymerase chain reaction ( PCR ) technique .
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结果:PCR和限制性核酸内切酶图谱分析均证实所获重组质粒中含有P1蛋白基因。
Results : Recombinant plasmid was proved to carry P1 protein gene by using PCR and endonuclease map analysis method .
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限制性核酸内切酶PstⅠ活性基团的研究
Studies on the Active Groups of Pst I Restriction Endonuclease
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方法应用PCR技术扩增T细胞受体γ链基因重排,选用四种限制性核酸内切酶消化扩增产物。
Methods After amplifying T-cell receptor γ chain gene rearrangement using polymerase chain reaction ( PCR ), we digested the products with 4 kinds of restriction enzyme .
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结果:(1)通过DNA测序和限制性核酸内切酶酶切鉴定证实,hMMP-12成功克隆到所需表达载体中;
Results : ( 1 ) The hMMP-12 was successfully cloned into the needed vector by enzymatic digestion and DNA sequencing ;
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质粒pBR322的体外扩建以及有关限制性核酸内切酶图谱
In vitro Enlargement of pBR322 and the Related Restriction Enzyme Maps
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人工核酸酶具有限制性核酸内切酶的高度专一性,能在人们预先设计的任何位点切割DNA,可应用于基因分离、DNA定位诱变、新的化学治疗等分子生物学领域。
Artificial nucleases have high selectivity just as endonucleases , they can cleave DNA at any locations that the chemists have designed , and they can be used in molecular biological areas such as isolation genes , DNA location inducers and new chemical therapy etc.
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通过PCR扩增狂犬病病毒SAD-B19株的G、N基因。将回收的目的片段用限制性核酸内切酶PstI进行酶切。
The G and N gene of rabies virus SAD-B19 strain were amplified by PCR , the target genes were collected and digested by Confine digestion enzyme of PstI .
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用X-gal平板及质粒图谱分析方法筛选重组克隆株,再用限制性核酸内切酶酶切图谱分析鉴定。
The recombinant clone was screened by X-gal plate and plasmid map , and identified by restricted enzyme mapping analysis method .
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方法应用聚合酶链反应、DNA测序和限制性核酸内切酶酶切等技术对15个AR-JP家系先证者的parkin基因进行突变研究。
Methods the polymerase chain reaction ( PCR ), DNA sequence analysis , and restriction enzyme digestion analysis were applied to check parkin gene mutations of 15 index patients from 15 families with AR-JP .
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其特点在于结合使用限制性核酸内切酶EcorI及纳米金标记的检测探针,能够很好的降低背景值并有效增强信号。
It features combined with restriction endonuclease ECoR I and nano-gold-labeled detection probe that can reduce the background of a very good value and effective way to boost the signal .
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利用限制性核酸内切酶PstⅠ在不同的有机介质中的催化反应,研究了水含量、相对粘度,介电常数和渗透压等介质的物化性质对PstⅠ原活性和星号活性的影响。
Through the catalyzed reactions of the restriction endonuclease Pst ⅰ in different organic medium , the effect of the four physico-chemical properties such as total water content , relative viscosity , dielectric constant and osmotic pressure on the prime and the star activity of Pst ⅰ was studied .
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单纯疱疹病毒基因组的限制性核酸内切酶分析
Analysis of Herpes Simplex Virus Genomes with Restriction Endonucleases
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芽孢杆菌中的限制性核酸内切酶
The restriction endonucleases from Bacillus
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我们从66株国内收集的芽孢杆菌中筛选出10株菌株,它们含有限制性核酸内切酶活力。
The activities of restriction endonucleases were found in10 out of66 strains of Bacillus isolated and examined in our country .