以纯化的融合蛋白免疫BALB/C小鼠，取其脾细胞，用传统杂交瘤技术与小鼠骨髓瘤细胞融合及次黄嘌呤、氨基蝶呤与胸苷选择性培养。
Fusion-protein of human Survivin was expressed and used for immunizing BALB / C mice . The spleen cells from immunized mice were fused with SP2 / 0 cells and selectively cultured with HAT medium .
氨基蝶呤