破伤风毒素

破伤风毒素C部分的克隆及在大肠杆菌中的表达

Cloning and Expression of Tetanus Toxin Fragment C in E.coli

破伤风毒素C片段基因克隆、重组表达及免疫原性研究

Study on gene cloning , expression and immunogenicity of tetanus toxin fragment C

破伤风毒素全基因片段PCR扩增、克隆和序列分析

Amplification , Cloning and Sequencing of Whole Gene Fragment of Tetanus Toxin

破伤风毒素及其AB片段对金鱼的毒力

Toxic Effects of Tetanus Toxin and Fragment AB on Goldfish

作者研究了破伤风毒素及其AB片段对金鱼和小鼠的毒力，发现16ng的毒素即可使金鱼麻痹和死亡；

The toxic effects of tetanus toxin and its fragment AB on goldfish and mice were de-scribed .

人源抗破伤风毒素单链抗体（ScFv）的原核表达、纯化及功能鉴定

Expression , purification and functional identification of the humanized monoclonal antibody against ScFv of tetanuss toxin

β型抗独特型单克隆抗体在抗疟免疫中的调节作用破伤风毒素及其AB片段对金鱼的毒力

Study on the Immune Regulation of Monoclonal Anti-idiotype Antibody ( Ab _2 β) Against Anti-sporozoite in P. yoelii Infected Mice Toxic Effects of Tetanus Toxin and Fragment AB on Goldfish

对抗破伤风毒素人源单克隆抗体G2、G6、G2+G6与精制破伤风抗毒素进行了中和效力比较试验。

The toxin neutralizing activity was detected by human MAbs G2 、 G6 、 G2 + G6 and horse anti tetanus antitoxin .

用ELISA间接法测得的TT，DT.DTP免疫豚鼠后的破伤风毒素水平，与用中和试验所得结果之间有很好的相关性。

The tetanus antitoxin levels of guinea pigs immunized with TT , DT and DTP were detected by indirect ELISA and compared with those by toxin neutralization test ( TNT ) . The result showed good correlation between them .

应用TFF微孔过滤澄清白喉、破伤风毒素培养液及超滤浓缩的试验

Experiments of Microporous Tangential flow Filtration in Clarification of Diphtheria and Tetanus Culture Suspension and Ultrafiltration in Concertration of Toxin Fluid

破伤风毒素培养滤液经PhenylSepharose疏水层析除去大部分杂质，再经DEAESephadex离子交换层析进一步纯化。

First most of impurity in the tetanus culture medium can be eliminated with HIC on Phenyl Sepharose , then tetanus toxin can be further purified by the ion-exchange chromatography ( IEX ) on DEAE Sephadex .

采用本研究建立的SABC-ELISA法检测炭肾灌洗前后血液中破伤风毒素的含量变化。

The blood samples were drawn before and after hemoperfusion for tetanospasmin measurement by SABC-ELISA , which was originally set up in this study .

用破伤风毒素免疫荧光法显示体外培养的神经细胞

Using tetanus toxin immunofluorescence to identify nerve cells in culture

人抗破伤风毒素免疫球蛋白国际标准品的协作标定

International Collaborative Standardization of International Standard of Human Anti-tetanus Immunoglobulin

层析法纯化破伤风毒素的试验研究

Study on purification of tetanus toxin by chromatography procedures

破伤风毒素的提纯及形成离子通道的特性

Purification of Tetanus Toxin and Formation of Ion Channels in a Lipid Bilayer

人源抗破伤风毒素单克隆抗体的中和活性和预防治疗作用

Neutralizing Activity and Preventive and Curative Effects of Human McAb to Tetanus Toxin

破伤风毒素及其片段的纯化

Purification of Tetanus Toxin and Its Fragments

结论炭肾灌洗能有效清除血液中的破伤风毒素。

Conclusions hemoperfusion with Charcoal kidney can effectively get rid the tetanospasmin out of blood .

结果实验兔使用炭吸附法后，血清中破伤风毒素的含量下降80%以上。

Results The content of spasmotoxin of the tetanus rabbit 's blood serum descended over 80 % .

破伤风毒素保护性抗原在毕氏酵母中的分泌表达

High-level Secretion Expression of Protective Fragment of Tetani Toxin in Pichia pastoris and Affecting Factors in Inducing Expression

用此方法，连续纯化五批毒素，均获得高纯度的破伤风毒素。试验证明破伤风毒素经疏水层析和离子交换层析可得到有效纯化。

We obtained a high purity of tetanus toxin in 5 consecutive batches by using the combined chromatography procedures .

为了获得高纯度的破伤风毒素，用疏水层析和离子交换层析纯化破伤风毒素。

To obtain high purity of tetanus toxin , purification of tetanus toxin was carried out by chromatography procedures .

目的对破伤风毒素全基因片段扩增、克隆和测序，以便有效利用。

Objective To amplify , clone and sequence the whole gene fragment of tetanus toxin and provide a basis for the application of it .

用破伤风毒素和异硫氰酸荧光素标记马抗破伤风血清可以特异地显示体外培养的神经细胞。

Tetanus toxin and FITC labelled horse anti-tetanus toxoid serum were applied to show the plasma membrane of cultivated chicken embryonic cerebral cortical cells .

白喉毒素总回收率分别为86%、76.6%，破伤风毒素总回收率为94.1%、92%。

The total recovery of toxin was 86 % , 76.6 % in diphtheria trials , and 94.1 % , 92 % in tetanus trials .

1μgTTC经4次免疫能诱导机体产生足够的抗体，保护动物免受破伤风毒素的攻击。

The immunization with 4 doses ( 1 μ g for each ) of the expressed tetanus toxin fragment C induced sufficient antibody to protect animals from challenge with tetanus toxin .

目的探讨炭吸附法清除破伤风毒素治疗成人重型破伤风的临床疗效，并通过动物实验证实炭吸附法清除破伤风毒素的效能。

Objective To explore the therapeutic effect of the adsorptive process of charcoal in tretment for serious tetanus , and to verify the efficacy of the adsorptive process of charcoal on spasmotoxin clearance by animal experiment .

应用双抗原ELISA夹心法检测破伤风抗毒素效力的试验

Detection of tetanus antitoxin titers by double antigen sandwich ELISA

其中早期气管切开、补充能量及鞘内注射破伤风抗毒素（TAT）患者疗效均较好。

But the patients treated with early tracheostomy , nutrition supplement and intrathecal injection of TAT recovered better .