荧光染色

大鼠海马DNA和RNA荧光染色强度。

The intensity of fluorescence staining of DNA and RNA in hippocampus of rats .

方法采用MTT比色法分析细胞存活率，Hoechst33258PI荧光染色法检测凋亡，流式细胞仪检测分析细胞凋亡率变化情况。

Methods Cell survival rate was measured by MTT assay and apoptosis was detected by Hoechst-PI fluorescence staining and flow cytometry .

PCR、DNA荧光染色法与培养法在支原体检测中的比较

Comparison of PCR , DNA Fluorescent and Cultivation Used for Detection of Mycoplasmas

结果细胞免疫荧光染色证实C组培养诱导的细胞为EC。

Results The immunochemical staining showed that the BMSCs were induced into EC in group C.

电镜、免疫荧光染色、westernblot等方式检测自噬水平的改变。

The change of autophagy was detected by electron microscopy , immunofluorescence , and Western Blot .

脾组织切片的免疫荧光染色结果表明，Ii链主要表达在细胞膜上。

Immunofluorescence staining showed that Ii proteins were expressed in the cell membranes of the splenocytes .

异质性的腹腔巨噬细胞的Fc和C3受体荧光染色观察Contributorstothisissue

SELECTED ABSTRACTS IN ENGLISH IN THIS ISSUE Heterogeneity of Fc and C_3 Receptors of Peritoneal Macrophages with Fluorescent Staining

对血清支原体进行检测时，应用直接培养法和DNA荧光染色法或PCR法同时联合应用，以提高对血清中支原体检测的准确性。

The cultivation combine with DNA fluorescent or PCR method for mycoplasmas detection can improve precision in batches calf serum .

内眼睑荧光染色线（Marx）线作为睑板腺功能的指示线

Marx line : Fluorescein staining line on the inner lid as indicator of meibomian gland function

结果免疫荧光染色检测见转染细胞内有HPVE6/E7表达；

RESULTS Indirect immunofluorescent staining showed the expression of HPV16E6 / E7 in transfected keratinocyte .

应用DTZ染色、Insulin免疫荧光染色对诱导的细胞进行鉴定。

Differentiated cells were detected by DTZ staining and Insulin immunofluorescence staining .

方法MTT法，AO/EB双荧光染色法，琼脂糖凝胶电泳，流式细胞仪方法。

METHODS MTT assay , AO / EB double staining , agarose gel electrophoresis , flow cytometry .

炎症相关因子在甲状腺内的表达：免疫荧光染色结果：（1）C组甲状腺均未见CD3阳性细胞。

Inflammation related gene expression in thyroid : Immunofluorescecce staining : ( 1 ) No CD3 positive cell in C group .

免疫荧光染色及RT-PCR检测，发现其表达多能性细胞及生殖细胞的标记，而且具有增殖能力，能够向三个胚层的细胞分化。

Immunofluorescence staining and RT-PCR determination found that the cells express pluripotency , proliferation and germ cells ' markers .

Hoechst33258荧光染色及Tunel法检测均可见典型的凋亡阳性细胞。

Typical apoptotic cells were detected by Hoechst 33258 staining and Tunel assay .

用荧光染色法观察了DM作用后大鼠分离脑细胞核的形态变化。

The morphological changes of nucleus by DM on rat dissociated neural cells were observed with fluorescent nuclear staining .

nestin荧光染色及诱导神经球分化鉴定神经干细胞。

The neural stem cells were identified by immunofluorescence staining for nestin .

免疫荧光染色检测神经干细胞特异性抗原神经巢蛋白Nestin的表达；

Expression of the neural stem cell marker nestin by immunoflurescence staining ;

本文用吖啶橙（AcridineOrange）荧光染色法进行了遗传毒理的研究，并取得了较好的效果。

Acridine orange fluorescent staining method was used on the studies of genetic toxicology with satisfactory results .

目的：评价聚合酶链反应（PCR），细菌培养，抗酸染色，荧光染色对结核病的诊断价值。

Objective : To evaluate the diagnostic value of polymerase chain reaction ( PCR ), culture , Semar acid fast staining , fluorescent staining in tuberculosis .

流式细胞仪技术及吖啶橙荧光染色观察NIM与MMC联合应用对人胃癌细胞SGC7901细胞凋亡影响。

MMC-c-induced apoptosis was determined by Acridine Orange staining and flow cytometry .

抗人血管性假血友病因子（vWF）免疫荧光染色显示，大量EPC参与肿瘤血管生成。

Immuno-fluorescent staining showed many human vWF antigen-positive endothelial cells being involved in the tumor vascularization .

DNA电泳分析和AO／EB荧光染色形态学检查表明，这三个肿瘤细胞株均存在自发性凋亡。

The spontaneous apoptosis was demonstrated in cell line of all stage of progression by both DNA agarose gel electrophoresis and morphology .

LTD组TL的浓度与泪膜破裂时间呈正相关，与荧光染色分数呈负相关。

TL concentration correlated positively with tear film break up time and negatively with fluorescein staining scores in patients with LTD.

结果表明，应用这种方法可以很明确的看到DNA、脂类及蛋白质特征峰在细胞中的分布情况，并且通过荧光染色验证了成像系统的可靠性。

The results show that , the distribution of DNA , lipid and protein can be exhibited by this Raman mapping system , and the reliability is validated by the fluorescence staining .

细胞凋亡荧光染色法在检测TIL细胞毒效应中的应用

A new approach for measurement of TIL cytotoxicity using apoptosis fluorescence staining assay

采用吖啶橙（AO）荧光染色法及流式细胞仪检测心肌细胞凋亡；

Acridine orange ( AO ) staining and flow cytometer based on PI staining were used to detect apoptosis .

采用ELISA和血凝抑制试验（HI）检测腹水mAb的效价；采用ELISA、HI、免疫荧光染色（IF）及westernblot鉴定mAb的特异性。

The specificity of the mAb was characterized by ELISA , HI test , indirect immunofluorescence ( IF ) staining and Western blot .

双重荧光染色显示，部分Bax及caspase&3蛋白阳性细胞同时可见凋亡阳性染色。

The Bax positive cells were fewer than caspase-3 . Double staining for apoptosis and caspase-3 or Bax demonstrated in some cells .

PKH26荧光染色的骨髓MSC在黑色素瘤组织内可以诱导分化、分裂增生参与肿瘤的血管形成；

In the melanoma , the PKH26-stained MSC induced differentiation and proliferation and then functioned in the tumor neovascularity .