转录起始位点

分析了两个启动子的转录起始位点、-10区和35区序列以及调控蛋白在启动子上的转录结合位点序列，预测其表达均为σ~（70）因子依赖型。

We predicted that the two promoters are α ~ ( 70 ) - dependent promoters , and predicted the transcriptional start site , the - 10 and - 35 hexamers , regulator-binding sequences by some software .

5′RACE分析确定其转录起始位点是269nt处的A。

Transcriptional initiation site A , which is at the 269 nucleotide , was preliminarily determined by using 5 ′ RACE method .

分离植物目的基因全长cDNA和启动子的新方法&快速定位转录起始位点（RITIS）（英文）

Isolation of Full-length cDNA and Promoter of Target Gene from Plant by Rapid Identification of Transcriptional Initiation Site ( RITIS )

通过RT-PCR的方法确定OsGCS基因的转录起始位点可能位于翻译起始位点（ATG）上游211bp处。

The putative transcription start site ( TSS ) confirmed by RT-PCR was located 211 bp upstream of the translation start codon ' ATG ' ( Fig.5 ) .

该方法通过对转录起始位点（TSS）信息，调控区、编码区组成成分特征信息及CpG岛相关信息的综合来预测人类基因组启动子。

The method makes predictions of promoter by combining information about transcription start sites ( TSS ), compositional features in coding and regulatory regions and CpG islands .

通过PCR方法对克隆的两个启动子进行定点突变，使转录起始位点上游-137bp处A突变为C，得到两个突变启动子（IPMl、IPMs）。

By artificially changing A to C at - 137 bp site upstream from transcription start point of cloned promoter , two site-mutation promoters , IPM s ( 603 bp ) and IPM 1 ( 900 bp ) were created .

首先利用RT-PCR和引物延伸方法确定了hb1f（nr5a2）基因的转录起始位点，并通过序列缺失方法鉴定出hb1f（nr5a2）基因的核心启动子。

We determined the transcription start site ( TSS ) of hb1f ( nr5a2 ) gene via RT-PCR and primer extension and then identified the promoter of hb1f ( nr5a2 ) gene , which exhibited high hepatocyte specific activity .

与其它测定基因转录起始位点的方法相比，RLM-RACE方法更快捷、简便、准确。

RLM-RACE ( RNA ligase-mediated rapid amplification of 5 ′ and 3 ′ cDNA ends ) is more rapid and simple comparing with the common methods for determining gene transcription initiation sites .

但对核小体结合模体的相对偏好性进行比较后发现，转录起始位点、转录终止位点、起始密码子和终止密码子附近±500bp的序列所包含的偏好的核小体结合模体模式数各不相同。

Comparison of relative preference of nucleosome binding motifs shows that the mode number of preferred nucleosome motifs in ± 500bp region around the transcription start sites , transcription termination sites , start codon and stop codon is different .

实验结果表明，与正常脱硫基因启动子活性相比，脱硫基因转录起始位点上游300bp启动子序列具100℅活性，150bp为42%，小于75bp活性为0。

The results showed that the desulfurization gene transcription start site upstream 300 bp promoter sequences with 100 % activity , 150 bp to 42 % , less than 75 bp for 0 , compared with the normal dsz promoter activity in strain R-8 .

基于滑动窗口的原核转录起始位点计算定位方法

Computational location of transcription start sites in prokaryotic genome based on sliding window

目的初步了解STK11/LKB1的转录起始位点信息，分析其可能的候选启动子区段，为以后进行STK11/LKB1的转录调控研究作准备。

Objective To map the approximate transcription initiation site of STK11 / LKB1 and identify its potential promoter region .

该算法首先根据转录起始位点可能出现位置的先验信息合理确定搜索范围。

This localization algorithm firstly limits the rational searching ranges in genomic sequences based on the prior information of TSSs occurrence .

核糖体蛋白基因转录起始位点附近碱基信息分析汉坦病毒包膜糖蛋白糖基化位点突变体的构建

Analysis of Base Information Round the TSS of Ribosomal Protein Genes ; Construction of N-linked glycosylation site mutants of envelope glycoprotein genes of hantavirus

距起始密码子ATG77bp处的T碱基为可能的转录起始位点。

Results showed a base T , 77 bp away from the initiation codon ATG for translation , was the possible initiation site for transcription .

分析的功能位点包括：转录起始位点，转录终止位点，起始密码子，终止密码子，内含子和外显子连接处等。

Analyzed functional sites contain transcription start sites , transcriptional termination sites , start codon , stop codon and junction sites of intron and exon .

这说明它们是一个基因的两个转录本，使用了不同的转录起始位点。

It hints that HSEI and HSEII are two transcripts of HSE gene transcribed from the different transcription start sites .

前人实验结果显示转录因子的结合位点多分布在基因转录起始位点上游区域。

Identification of transcription factor binding sites is an important step towards the understanding of the transcription regulation .

转录因子一般是与DNA上游序列特别是临近基因转录起始位点结合的蛋白质，通过调节或抑制转录机制来调节基因的表达。

Transcription factors are the protein normally binding the upstream DNA sequences , especially near the transcription start site . They regulate the expression of protein by regulating the transcription mechanism or inhibiting transcription .

看家基因转录边界和翻译边界区域，核小体结合模体在转录起始位点附近序列上出现频数最高。

In transcription and translation boundary region , nucleosome binding motifs show the highest frequency in the vicinity of the transcription start site .

转录因子结合位点的长度通常为5到20bp，相对于基因的转录起始位点（transcriptioninitialsite，TIS）有多种位置。

TFBS is usually 5 to 20 bp in length and situated variously relative to the transcription initial site ( TIS ) of a given gene .