读码框

第二种剪切方式得到一个短的cDNA，读码框只编码38个氨基酸。

In the second splicing pattern , the cDNA has a short open reading frame which encodes a protein of 38 AA .

之后，这些小的反义RNA可能会在RNA拼接中发挥作用，如在选择压力下修复基因组中一个区域，抑或在丧失或损坏一个模块后恢复一个读码框。

Such small antisense RNAs may later have gained a role in RNA editing , possibly under selective pressure to repair a region or restore a reading frame after loss or erosion of a module .

结果重组的融合蛋白表达载体经酶切、PCR和DNA序列测定证明构建正确，读码框无移位。

Results The recombinant fusion protein expression vector was verified correctly by enzyme digestion , PCR and sequence analysis .

开放读码框末端有由TAA组成的终止密码子。

The termination codon was composed of TAA .

方法用巢式聚合酶链反应（nestPCR）技术对淮阴地区220名专业献血者进行血清中输血传播病毒（TTV）的检测，并在PUC18中对其开放读码框2（ORF2）基因进行了克隆。

Methods TTV DNA in sera from 220 blood donors in Huaiyin was detected by nest polymerase chain reaction ( nest ? PCR ) .

PCR产物经过双酶切后按照正确的读码框顺序克隆到毕赤酵母表达载体pPICZαA中；

PCR products was inserted into the plasmid pPICZ α A after digested by the Xho ⅰ and Xba ⅰ restriction enzymes according to the correct reading code frame ;

预测和注释了45个开放读码框（ORF），分析了DNA复制基因、衣壳蛋白和DNA包装基因、侵染相关基因。

Putative forty-five open reading frames ( ORF ) were annotated , homology analysis were used to assign possible functions for the genes , including , DNA replication , synthesis of capsid protein , DNA packaged genes , and infection-associated genes .

经测序分析，最终在8个棉花品种中得到74条具有完整开放读码框的棉花RGAs。

The clones are sequenced and 74 RGAs with complete open reading frames ( ORF ) from eight varieties of cotton are finally obtained .

基因盒往往由一个编码抗生素抗性的开放读码框（ORF）和一个整合位点attC组成。

One gene cassette usually contains a open reading frame encoding antibiotic resistance and a specific recombination site called attC ( or 59-base element ) .

且基因起始端Kozak序列完全，基因终止子已经突变、读码框正确。

In the gene start area , there was an intact Kozak sequence . Terminator of gene was mutated and the reading frame was correctly identified .

通过PCR扩增和PCR产物直接克隆，将黄杆菌质粒上的一段长达1137bp的opd基因进行扩增及克隆，然后按正确的读码框亚克隆于表达载体pET28b（+）上，转化宿主菌E。

The 1137 bp DNA fragment encoding organophosphorus hydrolase was amplified with PCR , then directly cloned into pGEM-T vector . Plasmid pET-opd was constructed by subcloning the opd gene into plasmid pET28b ( + ) in correct reading frame .

目的：构建马动脉炎病毒读码框ORF5、ORF6、ORF7主要结构蛋白基因全长片段的克隆载体及汉逊酵母穿梭载体，并获得重组工程菌，为下一步酵母表达重组目的蛋白奠定基础。

Objective : To construction the cloning and yeast expression vectors containing ORF5 、 ORF6 、 ORF7 structural protein gene of equine arteritis virus and lay foundation for expressing these recombinant protein .

利用高保真酶扩增特性调整基因读码框的方法

A Convenient Method for Condon Adjustment by Using High-fidelity DNA Polymerase Amplification Property

该克隆含有1个630bp的开放读码框。

The whole cDNA clone was 2 048 bp in length and contains a 630 bp open reading frame .

开放读码框内有一个由123个核苷酸组成的重复单位，此重复单位在开放读码框内重复5次。

The open reading frame contained tandem repeating unit of 123 bp which appeared 5 times in this clone .

结果表明，在烟草原生质体中，C1+2基因的启动子活性最强，并受到C1+2读码框编码产物的抑制；

From tobacco protoplasts , strong promoter activity was observed for the promoter of C1 + C2 gene which was repressed by coelectroporation of C1 + 2 ORF construct .

有8.6%（及1.7%）的序列的预测读码框与南方中心预测的相同，但长度短于（及长于）南方中心注释结果；

8.6 % ( and 1.7 % ) of all of the sequences with the same reading frame as South Center s prediction was short ( and long ) in length .

该数据库包含了所有相位兼容的全新外显子-外显子组合情况，并依据相应读码框将序列翻译成氨基酸序列进行储存。

It represents all novel exon-exon junctions with compatible phases , and nucleotide sequences were translated into amino acid according to corresponding reading frames , and then stored them in a database file .

NtLEA7-3cDNA全长为1267bp，该序列含有一个969bp的完整开放读码框，编码322个氨基酸，蛋白质理论分子量约为35.7kDa，等电点为4.86。

The cDNA was 1 267 bp containing a 969 bp open reading frame which was deduced to encode a peptide of 322 amino acids whose predicted molecular mass was 35.7 kDa and isoelectric point was 4.86 .

其中一条的长度为2267bp，包括1509个碱基的开放读码框，编码503个氨基酸，分子量为57.6kDa。

One cDNA sequence , 2267 base pairs in length , contained an open reading frame of 1509 base pairs and it encoded a polypeptide of 503 amino acid residues with a predicted molecular weight of 57.6 kDa .