载体构建

加工番茄PG基因片段的cDNA克隆及表达载体构建

Polygalacturonase Gene Fragment cDNA Cloning of Processing Tomato and Antisense Plant Expression Vector Construction

桃果实中ACC合酶基因克隆及基因沉默载体构建

Cloning of ACC Synthase Gene and Vector Construction of Gene Silencing from Peach

IκBα突变体的真核表达载体构建及表达

Construction and Expression of Eukaryotic Expression Vector for I Kappa B Alpha Mutant Gene

乙型肝炎病毒X基因酵母表达载体构建及表达

Cloning and expression of hepatitis B virus X gene in yeast

3利用新型载体构建基因组文库以进行DNA大片段的敲除。

Establishment large chromosome fragment deletion on the basis of genomic library constructed with novel cosmid vector .

电泳图谱结果呈现预期的DNA条带，说明双基因RNA干扰载体构建成功。

Polyacrylamide gel electrophoresis results shows the expected DNA bands , indicating two-gene RNA interference vectors constructed successfully .

反义人类端粒酶RNA逆转录病毒载体构建及其对结直肠癌细胞的抑制作用

Construction of antisense human telomeric RNA retroviral vector and its inhibition on colorectal cancer cells

Epstein-Barr病毒DNA多聚酶基因扩增和表达载体构建

Amplification and Construction of Expression Vector of the Epstein-Barr Virus DNA Polymerase Gene

乙肝病毒前S1基因与截短C基因穿梭表达载体构建

Construction of Shuttle Expression Vector of Pre-S1 Gene and Truncated Core Gene of Hepatitis B Virus

Bt基因甜菜叶绿体转化载体构建及毒蛋白表达

Vector Construction with Bt Gene for Sugar Beet Chloroplast Transformation and Expression Toxin-protein

大鼠金属蛋白酶组织抑制物-1反义基因TA载体构建及其在肾小球系膜细胞表达的实验研究

Expression of antisense TIMP-1 gene TA cloning vector in rat glomerular mesangial cells

本文主要介绍有可能用于基因工程疫苗的DNA及RNA病毒载体构建及其应用。

Here , we mainly discuss the construction and application of DNA , RNA vectors which can be potentially used for delivery vaccine .

高Lys蛋白和高Met蛋白基因双价表达载体构建及其转化水稻

Construction and Transformation of Met-rich Protein and Lys-rich Protein Genes Co-expressing Vector in Rice

西伯利亚蓼GST和CS基因双价载体构建及转酵母和烟草研究

Construction of Vector with GST and CS Dual-gene from Polygonum Sibiricum and Its Transformation to Yeast and Tabacco

目的研究以减毒鼠伤寒沙门菌为载体构建的重组幽门螺杆菌（Hp）疫苗诱导小鼠产生保护性免疫应答的机制。

Objective To study the protective immune response induced by attenuated Salmonella typhimurium expressing Helicobacter pylori ( H. pylori ) antigens in mice .

FasL质粒载体构建及在小鼠心脏移植中抗排斥反应的研究

The Research on the Construction of FasL Plasmid Vector and Its Anti-rejection Effect in Mice Heart Transplantation

口蹄疫病毒结构蛋白P1基因植物表达载体构建及鉴定

Construction and Identification of Plant Expression Vector Foot and Mouth Disease Virus ( FMDV ) P1

抗菌肽cecropinP1基因表达载体构建与表达

Construction and expression of Porcine Cecropin P1 expression vector

结果经过PCR、限制性酶酶切鉴定，以及DNA序列分析，证实此两条同源臂为包含小鼠β2m前三个外显子在内的基因片段，证明载体构建成功。

The targeting vector was identified by PCR , restriction endonuclease and sequence analysis . Results The mouse β 2m gene targeting vector was successfully constructed .

BNYVVCP基因的克隆及载体构建

Cloning of cDNA for BNYVV CP Gene and Construction of Transforming Vector

tRNA~（val）-CTE复合核酶真核表达载体构建

Construction of eukaryotic vector of tRNA ~ ( val ) - CTE hybrid ribozyme

KDR启动子驱动的TNFR逆转录病毒载体构建及其靶向表达

The construction of KDR promoter drive TNFR retrovirus vector and its target expression

乙肝病毒HBx基因克隆及其转基因鼠表达载体构建

Clone of Hepatitis B Virus HBx and Construction of Mouse Liver-specific Expression Vectors

V3环缺失的HIV-1ADA株包膜表达载体构建及鉴定

Construction and identification of an expression vector of HIV-1 ADA env with V3 loop deletion

淋球菌孔蛋白PIB和PIA融合蛋白真核表达载体构建及体内表达

Construction and in Vivo Expression of Eukaryotic Recombinant Plasmid pCI-PIB-PIA

缺失GDD保守区的黄瓜花叶病毒复制酶基因的克隆及植物表达载体构建

Cloning of GDD deleted replicase gene of Cucumber mosaic virus and construction of its plant expression vector

利用λExCell载体构建了枸杞叶片cDNA文库，文库滴度为2.78x10~5pfu／ml，重组效率为88.1%。

CDNA library of Lycium barbarum was constructed , whose recombination rate was 88.1 % and the capacity of library was 2.78 × 10 ~ 5 pfu / ml.

利用毕赤酵母表达牛精蛋白的研究2.牛Myostatin活性肽编码区基因的克隆突变及其真核表达载体构建

Expression of Bovine Protamine in Pichia Pastoris 2.Cloning of Mutant Bovine Myostatin Active Domain Coding Sequence and Establishment of Its Eukaryotic Expression Vector

逆转录病毒载体构建的hNT-3基因在大鼠成纤维细胞中的表达

Stable Expression of Retroviral Constructed Gene of hNT-3 in Rat Primary Fibroblast Cells

主要内容如下：1.NA基因和Mx基因的克隆、序列分析及重组真核双表达载体构建。

The main research contents are as follows : 1 . Cloning , sequence analysis of NA and Mx genes , construction of recombinant eukaryotic co-expression vector .