重组质粒

含有马立克氏病病毒糖蛋白B基因重组质粒的构建及其鉴定

Construction and identification of recombinant plasmid containing the glycoprotein B gene of MAREKS'DISEASE virus

对重组质粒DNA进行纯化，并对目的基因片段进行核苷酸序列分析。

The recombinant plasmid was purified and the target DNA fragment was sequenced .

通过质粒双酶切和DNA测序证实该重组质粒构建正确。

This was confirmed by cleavage of restriction endonuclease and DNA sequencing .

对重组质粒进行核酸序列测定，并对N基因碳末端456个核苷酸进行序列系统树分析。

The three new plasmids were sequenced and analyzed with gene tree .

重组质粒DNA中插入子的核苷酸序列分析

Nucleotide sequence analysis of inserts in recombinant plasmid DNA

杜氏利什曼原虫LACK基因真核表达重组质粒及其在真核细胞中的表达

Construction of LACK Gene Recombinant Plasmid and Detection of Its Expression in Eukaryotic Cell

通过酶切、Southern杂交鉴定重组质粒。

The positive clone was identified by digestion and Southern analysis .

废弃重组质粒DNA热处理效率的环境影响因素

The environmental factors affecting the efficiency of thermo-treatment for disposal of waste plasmid DNA

碱裂解法提取重组质粒DNA及PCR验证

Using Alkaline Lysis Method for Recombine Plasmid DNA Extraction and PCR Verification

用酶切和PCR的方法对重组质粒进行鉴定。

Used restrict enzyme and PCR to verify the reconstructed plasmid .

淋病奈瑟菌孔蛋白B原核重组质粒的构建、表达与蛋白鉴定

Construction of Neisseria gonorrhoeae Porin B Plasmid Recombinant and Its Expression in E.coli and Identification of Fusion Protein

PCR和测序鉴定结果证明，重组质粒构建成功。

The recombinant plasmids were identified by PCR and sequencing .

重组质粒DNA的浓度为8μg/100mL～10μg/100mL培养瓶；

The concentration of the recombinant plasmid DNA is about 8 to 10 μ g per flask ;

扩增产物经纯化后直接克隆到PMD18-T载体系统中，经PCR及酶切法鉴定，所得的重组质粒中含有495bp的片段。

After purification , the DNA fragment was cloned into PMD-18T vector directly .

经DNA测序，证明重组质粒序列完全符合预期设计。

DNA sequencing for the recombinant plasmid demonstrated the obtained fragment are completely identic with previous expected design .

经酶切鉴定，DNA测序证实重组质粒构建正确；

The recombinant plasmid pET-28a ( + ) - IFN α was identified by enzyme digestion and DNA sequencing .

含日本血吸虫脂肪酸结合蛋白（SjFABPc）基因DNA重组质粒pCD-SjFABPc在哺乳动物细胞中的表达

Expression of DNA recombinant plasmid containing gene coding for cytoplasmic fatty acid-binding protein from Schistosoma japonicum in mammalian cells

分别经双酶切、特异PCR及测序法对重组质粒进行鉴定。

The recombinants were identified with double GA endonuclease digestion , specific PCR and sequencing .

CpG寡脱氧核苷酸长链DNA的克隆及重组质粒载体的构建

Cloning and construction of recombinant plasmids containing long strand CpG oligodeoxynucleotides DNA

大量抽提重组质粒，测定其拷贝浓度，10倍稀释成梯度标准品，并进行荧光定量PCR检测分析。

Standard quantitative curves were constructed by real-time PCR detection with the series of plasmid standards .

利用分子生物学技术制备了重组质粒、体外转录RNA，通过鉴定后建立了相应的重组质粒DNA阳性参比品、体外转录RNA阳性参比品。

Recombinant plasmid and in vitro transcription RNA were prepared and identified through molecular biology techniques .

癌胚抗原cDNA真核表达重组质粒的构建

Construction of CEA - cDNA Eukaryotic Expression Recombinant Plasmid

用α－互补法筛选HPVDNA重组质粒

Selecting HPV-DNA recombinants by α - complementation

CPGDNA重组质粒对猪O型口蹄疫病毒抗原的免疫佐剂效应

Adjuvant Effect of CpG DNA Recombinant Plasmid to Antigen of FMDV in vivo

ELISA法测定不同浓度地塞米松作用24h后，转染了3种重组质粒的成纤维细胞的CAT表达量。

The effects of dexamethasone on 3 plasmids were determined by CAT ELISA .

目的：构建增殖诱导配体（APRIL）基因的重组质粒的标准品。

Objective : To construct the standard recombinant plasmids for APRIL gene and using for quantification .

将构建的重组质粒DNA分别转染真核细胞后用免疫荧光法可检测到外源蛋白的表达。

Then these recombinant plasmid DNAs were transfected into eukaryotic cells , respectively . The foreign proteins were expressed and detected by IFA .

并分别通过PCR、限制性内切酶酶切图谱和序列分析3种方法对重组质粒进行鉴定。

The reconstructed plasmid ( pGBX ) was identified by PCR , restricted enzyme map , and sequence analysis .

鸡胚接种鸡白介素18重组质粒对IBDVDNA疫苗免疫增强作用的研究

Immunoenhancement on IBDV DNA vaccine by embryo vaccination with plasmid DNA encoding chicken interleukin-18

检测结果表明：四个重组质粒均能表达NGF蛋白。

It shows that the four all can express NGF protein .