可变区基因

PCR扩增抗乙酰胆碱受体单克隆抗体重链可变区基因的反应条件

Amplification of the heavy chain variable region gene of a monoclonal antibody against acetylcholine receptor by polymerase chain reaction

结论：成功克隆抗跨膜TNF-？单克隆抗体C1轻链及重链可变区基因，经序列分析证明为一对功能性免疫球蛋白可变区基因。

Conclusion : We have successfully cloned light chain and heavy chain variable region gene of anti - TM-TNF - α monoclonal antibody C1 , which is confirmed to be a new pair of functional rearranged variable region gene by immunoglobulin sequence analysis .

抗CD4单克隆抗体可变区基因克隆及测序

Cloning and sequencing of the VH / VL genes of anti-CD_4 McAb

单克隆抗体可变区基因PCR扩增产物的直接测序

Direct Sequencing of PCR-amplified Monoclonal Antibody Variable Region Genes

细胞内PCR法克隆抗体可变区基因

Antibody variable genes cloning by in cell PCR

克隆的轻链可变区基因长度为336bp，属于小鼠K轻链可变区Ⅱ亚组。

The light-chain variable region was 336 bp that belongs to mouse κ light-chain subgroup ⅱ .

设计简并引物，用RT-PCR的方法分别扩增抗体重链和轻链可变区基因cDNA文库。

The cDNA gene library of VH and VL fragments were amplified by RT-PCR .

抗CD3鼠/人嵌合抗体基因的构建及表达I.CD3单抗重链可变区基因的克隆及序列测定

Construction and Expression of Murine Human Chimeric Antibody with Specificity for CD_3 Antigen I.Cloning and Sequencing of the V_H Gene

目的获取系列鼠抗人CD分子单抗的轻链及重链可变区基因并进行克隆和测序。

[ Abstract ] Objective To obtain the VH and VL variable region genes of murine McAbs to human CD molecule .

目的利用二次聚合酶链反应（PCR）扩增人动脉粥样硬化抗体全套可变区基因，并将其克隆到T载体进行测序验证。

Objective To amplify all immunoglobulin V genes of human atherosclerosis by PCR and to sequence the products of PCR cloned into T-vector .

利用PCR分别扩增MG7单抗的重链及轻链可变区基因，并通过&DNA连接子将二者连接起来形成MG7单链抗体基因；

The variable fragments of heavy and light chain were separately amplified and assembled into ScFvs with a specially constructed DNA linker by PCR .

一种高效扩增人T细胞受体α链可变区基因方法的建立优化重叠PCR法进行单链抗体基因扩增和点突变

A high-performance method for amplifying human T-cell receptor V α - encoding genes Study of Single Chain Fvs Splicing and Site-directed Mutagenesis Based on Optimal Overlap PCR

抗变形链球菌SAⅠ/Ⅱ单抗重链可变区基因克隆及序列分析

Cloning and Sequencing of Variable Region Gene of Heavy Chain of Monoclonal Antibody Against SA ⅰ / ⅱ of Streptococcus mutans

抗人CD3单抗重、轻链可变区基因的体外扩增、克隆和序列分析

Amplification , Cloning and Sequence Analysis of the Genes of V_H and V_K of an Anti-human CD3 Monoclonal Antibody

抗体轻链可变区基因真核表达框架及抗CD3鼠/人嵌合轻链基因的构建

Constructions of a universal eukaryotic expressing framework for Ig VL genes and an anti-CD3 murine / human chimeric light chain gene

抗癌胚抗原单克隆抗体可变区基因的克隆及鼠-人嵌合抗体基因在E.coli中的高效表达

The Cloning and Construction of a Murine-Human Chimeric Antibody with Specificity for Carcinoembryonic Antigen and the High Expression of the Chimeric Genes in E. coli

抗H1亚型猪流感病毒单克隆抗体的制备及重链可变区基因的克隆

Preparation of Monoclonal Antibodies Against Subtype H1 of Swine Influenza Virus and Cloning Variable Region of Heavy Chain Gene

通过对3株抗乙酰胆碱受体（AChR）的α－银环蛇毒素（α－BT）结合区抗体的配位特异性测定和可变区基因序列分析，探讨重症肌无力（MG）的发病机制。

The sequence analysis and paratope mapping of three antibodies against α - bungarotoxin (α - BT ) binding sites on acetylcholine receptor ( AChR ) were studied .

用RLM-RACE法克隆抗CD20单克隆抗体可变区基因及其信号肽基因

Cloning of variable region and signal peptide genes of anti-CD20 monoclonal antibody by RLM-RACE

鼠抗CD71单抗轻链及重链可变区基因的克隆和序列分析

Molecular cloning and sequence analysis of heavy-and light-chain variable region genes of anti-CD71 monoclonal antibody

结果重组质粒酶切鉴定表明，人源性抗HFRS病毒抗体可变区基因已与载体pSVgpt和pSVneo正确连接。

Results Variable region genes of human anti HFRS virus antibody were connected with the vectors pSV gpt and pSV neo by enzyme digestion .

结论本研究采用RT－PCT法扩增出轻链及重链可变区基因，并进行了序列测定和分析，为进一步构建嵌合抗体以及单链抗体奠定了基础。乙酰胆碱受体单链噬菌体抗体的筛选与鉴定

The study laid a foundation of further development of chimeric and single chain antibodies . Selection and analysis of single chain antibodies against acetylcholine receptor from phage antibody library

结论：抗HBV末端蛋白轻、重链可变区基因的克隆及表达为进一步探讨HBV感染患者的免疫治疗奠定了基础。

CONCLUSION : The light and heavy chain variable gene fragments of mouse anti-HBV-PT antibody are successfully cloned and expressed . These variable gene fragments of the antibody can be used for further immunotherapy studies of HBV infected patients .

HFRSV内影像型抗独特型人单抗重链可变区基因克隆

Gene Cloning of the Heavy-Chain Variable Region of an Internal Image Human Monoclonal Anti-idiotype Antibody against Hemorrhagic Fever Virus

在早期工作中，我们曾从一株单克隆抗体杂交瘤克隆出抗TNFα抗体的轻、重链可变区基因并在大肠杆菌表达了其单链抗体（ScFv）和Fab抗体。

In our early work , we cloned the variable genes of an anti-human TNF a monoclonal antibody from hybridoma cells secreting the antibody , and expressed its ScFv and Fab in E Coli .

抗乳腺癌人单抗CM-1重链可变区基因的序列测定与分析

Amplification and Sequencing of Heavy Chain Variable Region ( VH ) Gene of a Human Monoclonal Antibody ( CM-1 ) with Specificity to Breast Cancer

目的：从抗变形链球菌SAⅠ／Ⅱ单抗杂交瘤细胞系2B12F6中扩增克隆重链可变区基因。

Objective : To clone and sequence a immunoglobulin variable region of heavy chain ( VH ) from a mouse hybridoma 2B 12 F 6 , which produce monoclonal antibody against SA ⅰ / ⅱ of Streptococcus mutans .

同源比较法克隆单抗CAb-1重链可变区基因

Cloning of Gene Encoding Variable Region of Heavy Chain from McAb CAb-1 by Homologous Comparison Approach

目的获得鼠抗人CD71分子单抗的轻链及重链可变区基因。

Ohjective To clone heavy-chain and light-chain vedable region ( VH and VL ) gene of mouse-anti-human CD71monoclonal antibody ( mAb ) .

结论克隆的VH和VL基因符合功能性重排的鼠抗体可变区基因特征，为将来制备具有GPX活性的单链抗体提供了可靠的基因材料。

Conclusion The VH and VL genes have the character of mice antibody variable genes . These genes will be used for expression of single chain Fv with GPX activity .